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Citations for Roche :
251 - 300 of 6508 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Bioengineering 2023Quote: ... SOS1•RAS complex was formed by incubating SOS1 and RAS at a stochiometric ratio of 1:3 overnight at 4° with 20 mM EDTA and alkaline phosphatase (Roche). The complex was then purified by gel filtration ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed 2 × 5 min at 4 °C with cold 1 × PBS containing protease inhibitor cocktail (Roche, #4693132001), snap frozen and stored at −80°C until awaiting further processing ...
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bioRxiv - Bioengineering 2023Quote: ... The primary antibodies and respective concentrations used in this study are the following: 4’,6-diamidino-2-phenylindole (DAPI) (1:500, Roche), anti-Ki67 (1:100 ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Immunology 2024Quote: Cell lysates were prepared by lysing cells for 1 h at 4 °C in RIPA buffer supplemented with cOmplete protease inhibitor cocktail (Roche). The cell lysate concentrations were quantified using a BCA protein assay kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... solution then stained for 12 - 18 hours at 4°C in buffer containing 10% HISS and alkaline phosphatase-conjugated sheep anti-digoxigenin antibody (1:2000, Roche).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were first incubated with 5 µl anti-GFP antibody (in house) for 1 hour rotating at 4°C and subsequently mixed with 50 µl protein G-agarose (50% slurry, Roche) and incubated for 1 hour rotating at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated overnight at 4°C with a primary monoclonal rat anti-HA antibody (1:500; Roche, #11867423001) diluted in 3% BSA in PBS ...
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Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles functionbioRxiv - Cell Biology 2024Quote: ... parasite cultures were incubated for 20 min at 37 °C in RPMI containing 1 µg/mL 4’,6’-diamidine-2’-phenylindole dihydrochloride (DAPI) (Roche) or 2.5 µM Bodipy-TR-C5-ceramide (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 10% heat inactivated sheep serum (HISS) and incubated at 4°C with anti-DIG antibody (1:2000, Roche) in TBST with 1% HISS overnight ...
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bioRxiv - Plant Biology 2022Quote: ... 200 mM NaCl, 1 mM EDTA, 1%NP-40, 1 mM DTT, 10 mM MgCl2, 1 × protease inhibitor cocktail from Roche) for 2 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM NaF, 1 mM Na3VO4, 1 mM PMSF, protease inhibitor cocktail [1 tablet in 50 mL lysis buffer, Roche]). The whole cell extract was denatured ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EGTA and 1 mM dithiothreitol) with 1% protease inhibitor cocktail (04693116001, Roche) and diluted 1x with the 2x loading buffer (65.8 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... cells were washed twice with Buffer 1 (1× eBioscience Perm/Wash Buffer, 1× Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 uL of 1 mg/mL pyrophosphatase (Roche) was added to each reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-PARP1 1:1000 (1 835 238 Roche), anti-tubulin 1:5000 (B-5-1-2 Santa Cruz) ...
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bioRxiv - Cell Biology 2022Quote: ... anti-GFP (mouse, Roche AB_390913, Substrate 1:1); anti-actin (mouse ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of 1 mg/mL pyrophosphatase (Roche) was added to each reaction ...
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bioRxiv - Cell Biology 2023Quote: ... HA-HRP (Roche, 12013819001, 1:1,000-1:5,000), MPP6 (Atlas antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor). The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: A pTargeT™ vector was labeled by nick translation with digoxigenin-11-dUTP (Roche) (Byron et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The riboprobe was obtained by in vitro transcription using DIG-11-UTP (Roche, Germany) from cDNA fragments cloned in pBluescript II SK (Stratagene ...
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bioRxiv - Microbiology 2021Quote: ... T7 in vitro transcription was performed in presence of conjugated DIG-11-UTP (Roche) following the manufacturer instruction (1 mM of each dNTP ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used for staining were: rat anti-HA (Roche 11 867 423 001); rabbit anti-CLIMP-63 (CKAP4 ...
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bioRxiv - Biophysics 2021Quote: ... was amplified by PCR in the presence of Digoxigenin-11-dUTP (Roche Diagnostics, Switzerland) using primers 1 and 2 (Table S3) ...
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bioRxiv - Cancer Biology 2020Quote: ... and probes were labelled with DIG using Digoxigenin-11-dUTP (Roche, Burgess Hill, UK) and the BioPrime® DNA Labeling System (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... and probes labeled with digoxigenin-11-dUTP were detected using anti-digoxigenin rhodamine (Roche). The preparations were counterstained using DAPI and mounted in Vectashield ...
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bioRxiv - Microbiology 2022Quote: ... DNA was detected using a DIG-labelled probe (Digoxigenin-11-dUTP alkali-labile; Roche) and anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.4) containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche), and frozen at -70°C ...
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bioRxiv - Immunology 2023Quote: ... incubated with 50 μl of anti-digoxigenin POD fragments (Roche, 11 633 716 001) diluted 1:5000 in 0.3% BSA PBS for 1 hour at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... After the addition of 11 μl of 2Å∼ KAPA HiFi HotStart ReadyMix (7958927001, Roche) and 1 μl of 2 mM ISPCR primers (Picelli et al ...
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bioRxiv - Microbiology 2024Quote: ... 0.5% NP40 and a cocktail of protease inhibitors (#11-836-170-001 Roche Diagnostic). Samples were incubated for 30 min on ice and cleared by centrifugation at 16,000 x g for 10 min at 4°C) ...
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bioRxiv - Developmental Biology 2024Quote: ... and used as templates for synthesis of riboprobes labeled with Digoxigenin-11-UTP (Roche, distributed by MilliporeSigma ...
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bioRxiv - Genomics 2024Quote: Probe DNA was labeled with either digoxigenin-11-dUTP or biotin-16-dUTP (Roche) using a nick translation reaction ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1% SDS) and a cocktail of protease inhibitors (Roche Diagnostic, #11-836-170-001) and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled tissue from each brain region was suspended in 6 ml of 0.32 M sucrose homogenization buffer (4 mM HEPES, 0.1 mM CaCl2, 1 mM MgCl2, plus Roche protease inhibitor tablet) and homogenized with a Teflon homogenizer using 10 strokes at 900 rpm ...
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bioRxiv - Plant Biology 2021Quote: ... The digested DNA was separated in a 1 % agarose gel at 50 Volts for 72 hours at 4°C and transferred to a nylon membrane (Roche®) overnight ...
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bioRxiv - Biophysics 2022Quote: ... embryos were washed (4 X 10 min) in PBTx and blocked in PBT-B (1× PBS, 20% (v/v) western blocking reagent (Roche, 11921673001), 2 mM ribonucleoside vanadyl complex (NEB ...
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bioRxiv - Pathology 2020Quote: ... Coverslips were incubated overnight at 4 °C with a 1:100 dilution with one of the following primary antibodies: HA (Roche, #867423001), KDEL (Abcam ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... for 3-4 hours at room temperature followed by overnight incubation at 4°C in anti-DIG-AP Fab fragments (1:5000) (Roche 1093274). Embryos were washed with PBST followed by an alkaline tris buffer ...
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bioRxiv - Immunology 2021Quote: ... incubated over-night (o/n) at 4°C with primary antibody diluted 1:500 in 5% Bovine Serum Albumin Fraction V (Roche 10735086001) in TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was then discarded and the pellet was resuspended in 300 µL SDS buffer (4 % SDS, 10 mM EDTA, 25 mM TrisHCl pH 7.5, 1 mM PMSF, 1x Roche protease inhibitor) and incubated at room temp (10 minutes) ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at room temperature and incubated with an appropriate antibody overnight at 4°C: 1:3000 anti-DIG-AP (Roche 11093274910), 1:1000 anti-DIG-POD (Roche 11207733910 ...
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bioRxiv - Cancer Biology 2022Quote: Engineered monoallelic cell pellets (500 million cells/sample) were lysed at 4°C in 1% CHAPS (Roche Diagnostics, cat no. 10810126001) lysis buffer (pH 8.0 ...
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bioRxiv - Physiology 2024Quote: ... the samples were blocked with 5% skim milk in PBS-DEPC for 1 hour at 4°C and incubated with anti-DIG antibody (Roche Diagnostics) diluted 1:10,000 in the blocking buffer for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Zoology 2023Quote: ... Single staining was performed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP; 1:50 dilution in alkaline buffer; Roche Diagnostics). The cells were then stained overnight at room temperature.