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Citations for Roche :
2901 - 2950 of 6963 citations for Rat Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The libraries were quantified using the KAPA Library Quantification Kit (Roche) and pooled for sequencing in NovaSeq 6000 (one lane ...
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bioRxiv - Microbiology 2024Quote: ... along with a LightCycler 480 SYBR I Master kit (Roche, Switzerland) and Roche consumables ...
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bioRxiv - Immunology 2024Quote: ... Stranded mRNA libraries were prepared a KAPA mRNA HyperPrep Kit (Roche) and sequenced on the Illumina HiSeq 4000 platform (75bp single-end sequencing).
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bioRxiv - Evolutionary Biology 2024Quote: ... Sequencing libraries were produced using the KAPA HyperPrep Kit (Roche 07137923001) using 500ng of starting material for each library ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA libraries were prepared using the KAPA Hyperprep kit (Roche; KK8504). The cycle number for library amplification was empirically determined using SYBR Green I Nucleic Acid Gel Stain (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified with High Pure PCR Product Purification Kit (Roche) and analyzed by Digital Droplet PCR (Biorad) ...
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bioRxiv - Molecular Biology 2024Quote: ... After purification with the High Pure PCR Cleanup Micro Kit (Roche), through T-A ligation ...
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bioRxiv - Cell Biology 2024Quote: ... Released LDH was assessed using the Cytotoxicity Detection kit (Roche, 11644793001) to determine necrotic cell death ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted using High Pure RNA Tissue kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Library concentrations were quantified using the KAPA library quantification kit (Roche), bioanalyzed for quality control (Agilent 2100 Bioanalyzer) ...
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bioRxiv - Neuroscience 2024Quote: ... Digoxigenin (DIG)-labeled riboprobes were synthesized using a kit (Roche 11277073910). Sections were post-fixed in PFA 4% ...
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bioRxiv - Molecular Biology 2024Quote: ... quantified with KAPA Library Quantification Kit for Illumina (Kapa Biosystems, KK4873), and sequenced on a NovaSeq 6000 System with 2 × 150 bp reads on SP flow cell.
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bioRxiv - Biochemistry 2024Quote: ... DNA was purified again using High Pure PCR Purification Kit (Roche) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: DNA was isolated using High Pure PCR Template Preparation Kit (Roche) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and a KAPA Dual-Indexed Adaper kit (KAPA Biosystems, Wilmington, MA). The NGS library was quantified with a qPCR-based KAPA Library Quantification kit (Roche ...
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bioRxiv - Immunology 2024Quote: ... Final library quantification was completed using a Kapa kit (Roche KK4824) prior to sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were constructed using KAPA mRNA Capture Kit (Roche, Basel, Switzerland) and MGIEasy RNA Directional Library Prep Set (MGI ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μM dNTPs (with 3:1 mix of 7-deaza-dGTP:dGTP; dNTPs from New England Bio Labs, cat#N0446, 7-deaza-2’-deoxy-GTP from Roche, cat#10988537001), 0.8 M Betaine (Alfa Aesar ...
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: ... Size selection using SPRI-beads isolated low molecular weight DNA which was then PCR amplified using 2 × HiFi HotStart Ready mix (Roche Diagnostics) and Nextera Indexing Primers (Illumina ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.1 mM EDTA) supplemented with 10 mM 2-mercaptoethanol (BME) and four EDTA-free cOmplete protease inhibitor tablets (Roche Applied Science) prior to lysis using the Nano DeBEE High Pressure Homogenizer (BEE International) ...
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bioRxiv - Immunology 2021Quote: Total protein lysates from MDDC and cDC cultured for 1 h in the presence of media or individual or combined Poly I:C and 2′3′-di AM(PS) agonists were obtained using RIPA buffer containing 1% phosphatase and protease inhibitors (Roche Diagnostics). Subsequently ...
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bioRxiv - Developmental Biology 2019Quote: ... washed in HM, resuspended in lysis buffer (SDS 2%, Tris-HCl pH 8.0 100 mM containing a mix of protease (Roche complete cocktail) and phosphatase inhibitors (Biotools cocktail or lab-made cocktail ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was diluted 1:2 (20 µL reverse transcription reaction to 40 µL water) and 2 µL of diluted cDNA was amplified in the LightCycler ® 480 Instrument II (Roche) in a 20 µL reaction containing 10 µL LightCycler ® 480 SYBR Green I Master Mix (Roche ...
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bioRxiv - Immunology 2019Quote: ... containing recombinant IL-2 (obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc61), to give a final IL-2 concentration of 100 units/mL ...
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bioRxiv - Plant Biology 2019Quote: ... Total proteins from 120 seedlings were extracted with 150 µL of extraction buffer (50 mM Tris-HCl pH 7.4, 80 mM NaCl, 0.1 % Tween 20, 10 % glycerol, 10 mM dithiothreitol, 2× Protease inhibitor cocktail [11873580001, Roche], 5 mM PMSF). Prior to protein quantification ...
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bioRxiv - Cell Biology 2019Quote: ... 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F], and protease inhibitor cocktail [Roche, 000000011836170001) and incubated on ice for 25 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 cycles of primary PCR to amplify the region of interest was performed using 2 μL of DNeasy eluate (∼100–300 ng template) in a 5 μL Kapa HiFi HotStart polymerase reaction (Kapa Biosystems; for primers see Supplementary Data Set 1) ...
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bioRxiv - Immunology 2019Quote: ... the HEK cells were lysed with a non-denaturing lysis buffer (20 mM Tris, pH 8.0, 137 mM NaCl, and 2 mM EDTA) supplemented with a protease inhibitor (Roche, Basel, Switzerland). After 10 min of incubation on ice ...
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bioRxiv - Immunology 2021Quote: ... variable region) was used as template in a 25 µl PCR reaction with 2× KAPA HiFi Hot Start Ready Mix (Roche #KK2602) and 1 µl (10 µM ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480; Roche Diagnostics, Mannheim, Germany) with SYBR Green I dye and gene-specific primers (Supplemental Table S8) ...
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bioRxiv - Immunology 2021Quote: ... cells were maintained in media containing 10 IU/ml rhIL-2 (AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc). Day 6-9 post-activation ...
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bioRxiv - Microbiology 2021Quote: ... mgc2 adhesin and mgc2-like adhesin (list of primers used in Table 2) were made by real-time PCR (rtPCR) on LightCycler 96 thermocycler (Roche®). The LightCycler SYBR Green I Master reaction mix (Roche® ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant MYC was eluted from the beads using SUMO elution buffer (250 mM imidazole, 10% glycerol, 1X PBS, 2 mM DTT) + PIC (Roche 05056489001).
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bioRxiv - Cancer Biology 2020Quote: ... 20 mM imidazole), and 1X with SUMO wash buffer (3 mM imidazole, 10% glycerol, 1X PBS, 2 mM DTT) + PIC (Roche 05056489001). Recombinant MYC was eluted from the beads using SUMO elution buffer (250 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... lysed by sonication in ice-cold Tris buffer (50 mM Tris pH 8.0, 150 mM NaCl, 10 mM imidazole, 2 mM βME, complete protease inhibitors (Roche, Basel, Switzerland), 1 μg/ml DNase ...
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bioRxiv - Plant Biology 2021Quote: ... 90 mM KCl, 2 mM EDTA, 0.5 mM spermidine, 0.2 mM spermine, 0.5 mM EGTA, 1× Roche Complete protease inhibitor cocktail). Nuclei were isolated by sucrose density gradient centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... mouse Hepa 1-6 and rat Fao hepatoma cells at 75% confluency were incubated with a mixture of oleate and palmitate (ratio 2:1, total concentration 1.2 mM) coupled to FA-free Bovine Serum Albumin (BSA) (Roche Applied Sciences). All fatty acid stocks were initially reconstituted in absolute ethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 mM NaCl, 2 mM MgAc2, 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]). After 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours at room temperature with an anti-FL antibody conjugated with HRP (1/500, Roche Diagnostics) followed by reaction with TSA biotin reagents (Perkin Elmer ...
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bioRxiv - Cell Biology 2022Quote: ... Purified and digested DNA was ligated to PCR products at 16°C overnight in a final volume of 20 μl using 2 U of T4 DNA ligase and 1x ligation buffer (Roche, 10481220001). Plasmid DNA was transformed into TOP10 chemically competent cells (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... pellets were dissolved with 300 µl 1X PMPG buffer (1x PBS no Mg2+/Ca2+, 2% Empigen) containing protease inhibitors (Roche, 11873580001) and RNasin (Promega) ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5% Triton X-100, 100 mM NaF, 1 mM ortho-vanadate, 2 mM EDTA, and a protease inhibitor cocktail [Roche 11836153001]). Lysates were vortexed and cleared by centrifugation (15,000 x g for 15 min at 4°C) ...
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bioRxiv - Microbiology 2022Quote: ... Immunoreactivity in the lung tissues was assessed using a rabbit anti-SARS-CoV-2 Nucleocapsid pAb as the primary antibody (Sinobiological, China) and further processed using a Ventana Discovery Ultra (Roche, USA) system.
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bioRxiv - Molecular Biology 2019Quote: ... Dehydrated slides were then placed on coverslips containing 70 µL hybridization mix (10 mM Tris-HCl, 2% blocking reagent (Roche, #11096176001), 70% formamide and 0.1 µM Cy3 labeled (CCCTAA)3 PNA probe (PNA Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... primary or re-amplified WTA products) added to 1 μl Expand Long Template Buffer 2 (Expand Long Template PCR System, Roche Diagnostics), 1 μl dNTPs (10 mM each) ...
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bioRxiv - Biochemistry 2019Quote: ... and the resulting duplex was captured on 3 mg streptavidin-coated magnetic beads (Roche, rotation for 2 h at 37°C). After extraction with phenol-chloroform and precipitation with ethanol ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...