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Citations for Roche :
2901 - 2950 of 6900 citations for Rat Angiopoietin 2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
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bioRxiv - Systems Biology 2023Quote: ... HepG2s were washed once with 1X PBS then lysed in SDS lysis buffer (50 mM Tris HCl/2% SDS/5% glycerol/5 mM EDTA/1mM NaF/dH2O) supplemented with cOmplete Protease Inhibitor Cocktail Tablets (Roche #11836170001), Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich® #P5726) ...
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bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 2 μM MG-132 proteasome inhibitor (#S2619, SelleckChem) and cOmplete® ULTRA EDTA-free inhibitor cocktail (#5892953001, Roche). Approximately 0.1 ml of 0.6 mm glass beads were added and thoroughly mixed ...
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bioRxiv - Cell Biology 2022Quote: ... 200 mM NaCl; 2 mM MgAc; 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]) per 1 g of cell pellet and incubated for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The elution fractions were collected in tubes that were previously supplemented with 2 µl Protector RNase inhibitor (Roche Diagnostics, Karlsruhe, Germany). The elution fractions were split ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Immunology 2023Quote: ... coated with 2.5ug/mL aCD3 and re-stimulated for an additional 3 days in complete IMDM-10 supplemented with 50U/mL IL-2 (Roche 11011456001). For Th17 polarizations ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (used only for CDKN1A ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in mHBSS at a density of 107 cells/ml and pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM in a BSA-coated centrifugation tube ...
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bioRxiv - Microbiology 2024Quote: ... BmE cells were transfected with 2 μg of this recombinant plasmid and A3 helper plasmid using X-tremeGENE HP DNA Transfection Reagent (Roche, Switzerland), and the culture medium was changed after 6 h ...
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bioRxiv - Molecular Biology 2024Quote: Nuclei protein from MOLM-13-Cas9 cells transduced with either sgROSA or sgTOPORS-1 treated with 2 doses of DAC or vehicle over 24hrs was extracted in RIPA buffer supplemented with protease inhibitor (Roche, #04693159001). 10ug of protein was boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Genotyping of mice was performed using a mouse genotyping kit (Kapa Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche). PCR amplification was performed as follows ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA synthesis was performed using Transcriptor High-Fidelity cDNA Synthesis Kit (Roche) with 500 ng RNA per reaction ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: Total RNA was isolated from cells with High Pure Isolation Kit (Roche). For cDNA synthesis random nonamers ...
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bioRxiv - Microbiology 2020Quote: ... coli Novablue were extracted using the High Pure RNA Isolation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The P4 levels in the serum were measured using commercial Kit (Roche).
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bioRxiv - Developmental Biology 2021Quote: ... followed by cDNA synthesis using Transcriptor First Strand cDNA Synthesis Kit (Roche) then was stored at −20 °C ...
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bioRxiv - Developmental Biology 2021Quote: cDNA libraries were prepared using KAPA Stranded mRNA-seq kit (KAPA Biosystems) for the MZ/IZ/CZ bulk RNA-Seq experiment and the Universal Plus mRNA-Seq kit (Nugen ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified with High Pure PCR Product Purification Kit (Roche). After sequencing reaction ...
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bioRxiv - Microbiology 2019Quote: ... we prepared sequencing libraries using the KAPA HyperPrep kit (Roche, Basal, Switzerland) and sequenced on the Illumina HiSeq2500 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The templates were in vitro transcribed using DIG RNA labeling kit (Roche). In situ hybridization was performed as described in Pascual ...
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bioRxiv - Developmental Biology 2021Quote: ... Apoptosis was detected with the In Situ Cell Death Detection Kit (Roche) according to the product manual ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic libraries were prepared using the Hyper library construction kit (Kapa Biosystems). These libraries were sequenced to generate 150bp paired-end reads using Illumina NovaSeq 6000 with S4 reagents ...
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bioRxiv - Evolutionary Biology 2020Quote: ... which was then amplified using the KAPA HiFi PCR Kits (Roche, USA). The amplified products were classified into different fragments according to their sizes (1~2 k ...
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bioRxiv - Evolutionary Biology 2021Quote: ... UCE libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems), using up to 250 ng DNA (i.e. ...
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bioRxiv - Developmental Biology 2021Quote: ... and cDNAs transcribed with the Transcription First Strand cDNA Synthesis Kit (Roche) according to the manufacturer’s description ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Short-read libraries were constructed either using the KAPA HyperPrep kit (Roche), or the NGS DNA Library Prep Kit (Westburg) ...
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bioRxiv - Developmental Biology 2020Quote: ... and reverse transcribed with first Strand cDNA Synthesis Kit (Roche, Barcelona, Spain).
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were then quantified using the Kapa qPCR Library Quantitation kit (Roche) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were prepared using the KAPA Stranded mRNA-Seq Kit (Roche) and sequenced on an Illumina Novaseq 6000 with 50 bp paired-end reads (>20 million reads per sample ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL was performed using an In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Library preparation was performed using KAPA RNA HyperPrep Kit (Kapa Biosystems, KR1350) (input amount 500ng RNA ...
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bioRxiv - Developmental Biology 2022Quote: Libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems, KR0961) (input amount 10ng DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were prepared using the KAPA Stranded mRNA-Seq Kit (Roche) and sequenced on an Illumina HiSeq 4000 with 100 bp paired-end reads by the Duke University Center for Genomic and Computational Biology.
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bioRxiv - Molecular Biology 2020Quote: ... and cDNA synthesised using the Transcriptor First Strand cDNA Synthesis kit (Roche) with random hexamer primers ...
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bioRxiv - Molecular Biology 2020Quote: ... DIG-labelled probe prepared using PCR DIG Probe Synthesis Kit (#11636090910, Roche) was added and the membranes incubated for over 18 h ...
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bioRxiv - Molecular Biology 2020Quote: ... We used DIG RNA labelling kit (SP6/T7) (Cat. No. 11175025910, Roche) to synthesize probes according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and probe detection was performed using a luminescence detection kit II (Roche) according to the manufacturer’s protocol ...