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Citations for Roche :
2901 - 2950 of 7278 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... An equal volume of hypertonic lysis buffer (HEPES 20 mM, NaCl2 500 mM, MgCl2 1 mM, Glycerol 10%, DTT 1 mM, 1x Complete protease inhibitor [Roche]) was then added to the lysate ...
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bioRxiv - Neuroscience 2022Quote: Right hemispheres of 3 PS19 mice brains of identical ages ranging from 1 to 6 weeks and 1 year old were gently homogenized in 1:10 ratio w/v of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and resuspended in 0.5 mL lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg·mL-1 lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany) and incubated at 37°C for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-BALL (Gift from A. Herzig, IF 1:20, ChIP: 2μl) mouse anti-GFP (Roche, 11814460001, IF:1:50), mouse anti-Tubulin (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA supplemented with 1 × protease inhibitor cOmplete Mini EDTA-free mixture from Roche) and subjected to WB assay.
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bioRxiv - Molecular Biology 2021Quote: ... and then resuspended in 200 μL of SCE buffer (1 M sorbitol, 10 mM EDTA, 10 mM DTT, 100mM sodium citrate, 1 Roche mini-EDTA-free protease inhibitor tablet per 50 mL ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 mM KCl, 10 mM MgCl2, 1% Triton-X100, 100 ug/ml cycloheximide, 1 mM DTT, complete EDTA-free protease inhibitors – Roche) plus 500 uL acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... followed by sonication in 250 ml of 1% sodium dodecyl sulfate and 1× cOmplete protease inhibitor mixture (Roche, Indianapolis, IN) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The blocking solution was removed and replaced with antibody solution containing 1% heat inactivated goat serum and a 1:5000 dilution of anti-DIG-AP antibody (Roche). Slides were then incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in 1x maleic acid for 1h at RT and riboprobes were detected by incubating in anti-digoxigenin-AP antibody (1:2000 in 1% sheep serum, Roche) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Probes were detected by horseradish peroxidase (POD)-conjugated antibodies (anti-FITC-POD at 1/250 dilution, Roche; anti-DIG-POD at 1/500 dilution, Roche). Signals were amplified by biotin-conjugated tyramide (PerkinElmer ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in complete media (DMEM with 10% FBS and 1% antibiotics) supplemented with Collagenase-D (1 mg/mL; Roche) and incubated at 37°C for 30 min with shaking to form a single-cell suspension ...
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bioRxiv - Microbiology 2021Quote: ... Slides were incubated at room temperature in the following primary antibodies at 1:500 dilution in 1% BSA/1xPBS: rat anti-HA (Roche) and rabbit anti-αTubulin (Abcam) ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (1%vol/vol)) containing PMSF (final concentration 1 mM) and complete protease inhibitor cocktail (without EDTA, from Roche). A 500 μl volume of glass beads was added to the tubes ...
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bioRxiv - Biophysics 2022Quote: ... The pellet was resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterwards dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL® (SPEX SamplePrep LLC) ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted from adherent cells by scraping into extraction buffer (1× LysisM, 1× protease inhibitor cocktail, 2 x phosphatase inhibitor cocktail (all Roche), 2 mM sodium orthovanadate (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... islets were pre-incubated for 1 hour in KRBH buffer (described in in vitro calcium imaging) containing 1% w/v BSA (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± agonists in KRBH in a shaking 37°C water-bath (80 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Immunology 2021Quote: Cells were collected in RIPA buffer (0.5 M EDTA, 1 M Tris-Cl pH7.5, 1 M NaCl, 200 mM, Roche protease inhibitor) at 0h ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µl starch degradation mix (16.8 units ml-1 amyloglucosidase [#10102857001; Roche, Mannheim, Germany] and 12 units ml-1 α-amylase [#10102814001; Roche, Mannheim ...
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bioRxiv - Neuroscience 2021Quote: ... in sucrose lysis buffer (270 nM sucrose, 10 mM Tris-HCl, 1 mM NaHCO3, 1 mM MgCl2, protease inhibitor (cOmplete Mini, Roche), phosphatase inhibitor (PhosphoStop ...
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bioRxiv - Cell Biology 2021Quote: ... Mice tracheas were processed by peeling off the epithelium and digesting in a 1:1 mixture of DNAse II (Roche) and Liberase (Roche ...
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bioRxiv - Pathology 2020Quote: ... Tween-20 0.1%) for 1 hour and incubated overnight at 4 °C with the appropriated antibodies: HA (dilution 1:1000, Roche, #867423001), ß-tubulin (dilution 1:5000 ...
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bioRxiv - Synthetic Biology 2021Quote: The cell pellet was thawed and resuspended in lysis buffer (1×PBS containing 150 mM NaCl, pH 7.4, 0.5 mM TCEP, 0.1 mM PMSF, 1 × Roche complete protease inhibitors), and lysed in a cell disruptor ...
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bioRxiv - Microbiology 2020Quote: After adding 1 mL of PBS (pH 7.4) containing protease inhibitor (1 tablet per 10 mL, Roche cOmplete™, #04693159001) and 500 μL of 0.5 mm diameter glass beads ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were diluted to 1 ng/µl with double distilled H2O (ddH2O) and 1 ng was used for standard SYBR Green (Roche) RT-qPCR reactions on a LightCycler480 (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested by centrifugation and lysed in 1% Nonidet P40/1 mM EDTA/PBS containing Complete protease inhibitors (Roche), and nuclei were removed by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked in 10% inactivated sheep serum for 1 h followed by overnight incubation with 1:1000 anti-digoxygenin (DIG) antibody (Roche). The sections were washed in PBT and incubated with NBT/BCIP (Roche ...
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bioRxiv - Microbiology 2020Quote: ... and low-volume supernatants (90 μL media per well of a 48-well plate) were mixed 1:1 with 2× SDS/PAGE sample buffer containing Complete Mini EDTA-free Protease Inhibitor Mixture (Roche). In experiments where primary hMDMs were plated in a 24-well plate and infected with T4SS-Lp ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells transfected with sfGFP-TAOK2 WT and sfGFP-TAOK2 K57A were lysed with HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, Complete)) ...
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bioRxiv - Cell Biology 2021Quote: ... and lysed in HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, Complete), Halt Phosphatase Inhibitors (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... back skin was removed from mice (p0-p3) and placed in 1:1 Dispase II (Hoffman-La Roche, Basel, Switzerland):1X phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with 1 µL RNase A (Thermo) for 30 minutes followed by 1 µL Proteinase K (20 mg/mL, Roche) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μL 1 M spermidine, 500 µL 10% Triton X-100, and 10 ml glycerol in 38 ml dH2O, 1 Roche Complete Protease Inhibitor EDTA-Free (Millipore Sigma 11873580001)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysis buffer (150 mM NaCl, 1 mM EDTA, 50 mM Tris-HCl, 1% NP-40 with proteases and phosphatases inhibitors (Roche)) was added directly to the wells and cells were scraped and collected in pre-cooled Eppendorf tubes followed by sonification for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in 1% NP-40 lysis buffer (1% NP-40, 50 nM Tris HCl, 150 mM NaCl) supplemented with 1 × EDTA-free protease inhibitor cocktail (Roche) and Benzonase (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking solution was removed and replaced with antibody solution containing 1% heat inactivated goat serum and a 1:5000 dilution of anti-DIG-AP antibody (Roche). Slides were then incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26 ...
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bioRxiv - Neuroscience 2022Quote: ... brains were incubated with primary antibodies (1:200, chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913 ...
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bioRxiv - Neuroscience 2022Quote: HEK293T cells transfected with N-terminally sfGFP-tagged indicated variants of TAOK1 were lysed with HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, cOmplete). Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed in Western Blot (WB) lysis buffer (1% SDS, 10 mM Tris, 1 mM EDTA, phosphatase and protease inhibitors (Roche, 04906837001 and 04693159001 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 850 μL RIPA buffer (1×PBS, 1% IGEPAL, 0.5% Sodium Deoxy-cholate, 0.1% SDS, Roche Protease Inhibitor Cocktail) and added to the beads ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor, 1X Roche phosphatase inhibitor) followed by a 30’incubation on ice ...
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bioRxiv - Neuroscience 2023Quote: Cell pellets were resuspended in a lysis solution composed by 1 X Phosphate Saline Solution (PBS) supplemented with 1 X Complete Protease Inhibitor Cocktail (Roche) and then sonicated with a BioRuptor UCD-200 (Diagenode ...
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bioRxiv - Plant Biology 2023Quote: ... 1% [v/v] Triton X-100, 150 mM NaCl, 1 mM EDTA, 10% [v/v] glycerol, and 1× protease inhibitor cocktail [Roche]) by rotating at 4 °C for 1 h ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... Cell fraction was washed twice and resuspended into 30 μL with 1 × phosphate-buffered saline (PBS) and 1 × cOmplete proteinase inhibitor (Roche). Supernatant fraction was supplemented with 1 × cOmplete and reduced to 30 μL with vacuum centrifuge ...