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Citations for Roche :
2851 - 2900 of 6869 citations for Roundabout Guidance Receptor 1 ROBO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
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bioRxiv - Plant Biology 2024Quote: ... and then total proteins were extracted and protein levels were determined by immunoblot analysis with anti-GFP antibody (Roche).
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bioRxiv - Plant Biology 2023Quote: ... HA-tagged RIN4a and RIN4b expressed in soybean transgenic roots were detected using monoclonal HA-antibody (Roche Diagnostics, Germany) with or without HRP-conjugate and used in 1:2500 dilution ...
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bioRxiv - Biophysics 2023Quote: ... Applying voltage to the piezo element produced standing planar acoustic waves generating force applied to the polystyrene microspheres inside the chamber in the direction towards the top side of the chamber The AFS chip was first passivated by treatment with 0.05 mg/ml of anti-digoxigenin antibodies (Roche), followed by incubation with 0.2% BSA (Amresco ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG/HA-AGO2 expression and immunoprecipitation efficiency were determined by Western blot using anti-HA antibody (clone 3F10, Roche). RNA was extracted by adding Trizol reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with rat anti-HA antibody (clone 3F10, Roche), or rabbit anti-TgSkp1 affinity purified polyclonal antibody UOK75 [76],
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bioRxiv - Plant Biology 2024Quote: ... pH 7.5) and probed for 30 min with an alkaline phosphatase-labeled anti-digoxigenin antibody (Roche, catalog no. 11093274910). Membranes were washed twice for 15 min with washing buffer (0.1 M maleic acid ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910, lot 10520200 anti-fluorescein-HRP (1:2000, Roche/Sigma-Aldrich 11426346910 ...
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bioRxiv - Physiology 2022Quote: ... 2 mmol/l MgCl2, 10 mmol/l NaF, 1 mmol/l PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 30 min on ice and centrifuged at 20,000 g for 10 min at 4ºC ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM EDTA) supplemented with 1mM PMSF and 1 tablet of cOmplete Protease inhibitor (Roche, cat. No. 11 873 580 001) per 50 ml ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell pellet were thawed in 500 μl hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %, DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease) and incubated at room temperature for 15 mins (for nuclease activity) ...
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bioRxiv - Developmental Biology 2019Quote: Cell pellet were thawed in 300 μl hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %, DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease) and incubated at room temperature for 15 mins nucleic acid digestion ...
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bioRxiv - Cell Biology 2019Quote: ... 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F], and protease inhibitor cocktail [Roche, 000000011836170001) and incubated on ice for 25 min ...
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bioRxiv - Neuroscience 2020Quote: ... Rat pups were injected with BrdU (i.p. 50 mg kg-1 day-1 BrdU in saline; Roche Diagnostics, Indianapolis, IN, USA) for 5 consecutive days ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were homogenized in 2 mL co-IP lysis buffer (20 mM Tris-HCl, pH = 7.6; 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Nonidet P40 Substitute; protease inhibitor cocktail [Roche]; PhosSTOP [Roche]), using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2021Quote: ... mice and lysed on ice for 30 min in protein lysis buffer (1 mM Na3VO4, 1 mM NaF, complete protease inhibitor cocktail (Roche, USA), 1% Triton X-100 in PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... 3 volumes of lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM KCL, 1 % Triton X100, Protease inhibitor 1 X [Roche], NEM 20uM) was added ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in 100 μl of protein breakage buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA pH 7.5, 2.75 mM DTT, 1 x Roche EDTA-free protease inhibitors) and one volume of glass beads (0.5mM zirconia/silica glass beads ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, pH 8.0; 1 mM EGTA, pH 8.0; 0.65% NP-40; 1 mM DTT, 1x Roche Complete protease inhibitors cocktail). Nuclei were pelleted at 3,000 x g for 20min at 4°C and cytoplasmic fraction was removed ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 mM NaCl, 2 mM MgAc2, 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]). After 30 min ...
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bioRxiv - Immunology 2022Quote: ... counted and lysed in m-RIPA buffer (1% NP40, 1% sodium deoxycholate, 150mM NaCl, 50mM Tris pH 7.5, Complete protease inhibitors; Roche, Basel, Switzerland). CANX and T6BP were immunoprecipitated using anti-CANX antibody PA5-34754 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were re-suspended in 1 mL lysis buffer (5 mM EDTA, 1% NP-40 in PBS) supplemented with 2X cOmplete™ inhibitor (Roche) and were sonicated until the lysate became turbid ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, 10 mM Sodium butyrate, 0.1% deoxycholate, 0.1% SDS, 1% Triton X-100, 1mM PMSF and 1 × Roche protease inhibitor Cocktail). The obtained chromatin was sonicated into DNA fragments between 200-500 bp using Diagenode Bioruptor (high setting ...
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bioRxiv - Physiology 2020Quote: ... in lysis buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was again discarded and 0.6 ml Storage buffer (10% glycerol, 1 mM EDTA, 10 mM TrisHCl pH 7.5, 25 mM NaCl, 1 mM PMSF, 1x Roche protease inhibitor) was added on top of the pellet ...
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bioRxiv - Cancer Biology 2020Quote: ... 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1 mM PMSF, Roche protease inhibitors 11836170001) for 10 min at 4°C with rotation ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were lysed in the lysis buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1 % Triton, 1 x EDTA-free Roche protease inhibitors) and incubated on ice for 30 mins prior to a double sonication at 12 % for 3 seconds each (1 min on ice between sonications) ...
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bioRxiv - Microbiology 2021Quote: ... falciparum ACT1 (final dilution 1:500; a kind gift from Jake Baum) or rat anti-HA (1:1000; Roche, Basel, Switzerland) for HA-tagged GAP45 and AMA1 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 seconds each with intermittent cooling on ice ...
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bioRxiv - Immunology 2020Quote: ... 0.1% NP-40 (Nonidet P40, Applichem) and 1 pill of protease inhibitor EDTA-free (cOmplete Tablets, Mini EDTA free, Roche diagnostics) per 30 mL lysate ...
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bioRxiv - Biophysics 2019Quote: ... cells were harvested by centrifugation and resuspended in lysis buffer (20 mM Tris pH 7.5, 1 mM EDTA, 200 mM NaCl, 1 mM βMe, Roche protease inhibitor) and frozen ...
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bioRxiv - Microbiology 2020Quote: ... from persons with established infection (both ART naïve and receiving ART) with HIV-1 VL data (COBAS AmpliPrep/COBAS TaqMan® HIV-1 Test, v2.0, Roche Molecular Diagnostics) were included to evaluate the impact of VL on the performance of the ARCHITECT.STOP was a prospective study evaluating the recommended diagnostic algorithm for detection of acute HIV infection and linkage to enhanced partner services in New York City ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed twice in 1□ml Digitonin Buffer (20 mM HEPES-KOH pH 7.5; 150□mM NaCl; 0.5□mM Spermidine; 1×Roche cOmpleteTM; 0.05% digitonin), and then resuspended with CUTANA pAG-MNase in 50□μl Digitonin Buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 25 million cells were resuspended in 1 ml lysis buffer (1% SDS, 50 mM Tris-HCl pH 8, 20 mM EDTA, 1x cOmplete (Roche, 4693132001) protease inhibitor) ...
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bioRxiv - Immunology 2022Quote: Skin biopsies were collected and minced with sterile scissors before digestion in 1 mL of DMEM with 1 mg/mL collagenase D (Roche Diagnostics) for 3 hours at 37℃ ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad Laboratories GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM NaCl, 2 mM MgCl2, 10 mM NaF, 1 mM PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 45 min on ice and centrifuged at 20000g for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: The nuclei were resuspended in 250 µL of 5’ phosphorylation master mix (1X T4 PNK buffer, 500 U/mL of T4PNK, 1 mM ATP, 1 U/µL of RNAse inhibitor (Roche, 3335399001)) and incubated at 37°C while rotating at 800 rpm for 1 hour to phosphorylate the 5’ ends of RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed for 10 min on ice in 3 ml ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1, 1 mM PMSF, 1,5x Roche protease inhibitor mix). The DNA was sheared by sonication (4x 7 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM EDTA, 1 mM Na3VO4, 20 mM NaF, 0.5% Triton X-100, 1 × protease inhibitors complete EDTA-free from Roche, Mannheim, Germany). Total protein concentration of prepared cell extracts was measured by Bio-Rad protein assay (Bio-Rad laboratories GmbH ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.9, 50% glycerol, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 1 mM PMSF and Roche protease inhibitor cocktail), then 100 μl prechilled nuclei lysis buffer (10 mM HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche] in Maleic acid) for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... cut into small pieces with a scalpel and transferred into 1 ml collagenase-dispase solution (1 mg/ml in PBS, Roche #10269638001) for 10 min at 28°C ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris/HCl pH7.5, 1 mM EDTA, 300 mM NaCl, 1 mM DTT, 0.1% [v/v] Nonidet P-40/Igepal, Roche cOmpleteTM protease inhibitor) was added to grinded-frozen samples ...
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bioRxiv - Molecular Biology 2023Quote: ... extract was prepared using Co-IP buffer (50 mM Tris/Cl (pH=7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001), 0.5 mM PMSF) ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1% SDS) with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 X EDTA-free mammalian protease inhibitor cocktail (Roche Diagnostics, 11836170001). Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Cell Biology 2022Quote: ... 200 mM NaCl; 2 mM MgAc; 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]) per 1 g of cell pellet and incubated for 30 min at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...