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Citations for Roche :
2851 - 2900 of 3944 citations for Mouse anti Plasmodium vivax CSP PVC 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... brain sections were incubated with peroxidase (POD)-conjugated anti-fluorescein (Roche Applied Science ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots were incubated with anti-GFP (5 µg, Roche, # 11814460001, Germany) for GFP or HA11(5ug ...
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bioRxiv - Genomics 2022Quote: ... and the digoxigenin labelled probe detected with anti-digoxigenin antibody (Roche) and Alexa-Fluor 647 donkey anti-sheep antibody (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... except cleared lysates were incubated with anti-HA matrix (11815016001, Roche) (pre-blocked with 1% BSA-PBS for 1h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and horseradish peroxidase (HRP)-conjugated anti-fluorescein antibody (1/500, Roche) were applied ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed with antibodies: anti-hemagglutinin (HA) (3F10) HRP (Roche), anti-V5 (V2260 ...
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bioRxiv - Pathology 2023Quote: ... The expressed proteins were detected by using anti-HA-HRP (Roche) at a dilution of 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-HA antibodies were from Roche (clone 12CA5 for Western blotting) or from SantaCruz (clone Y11 for immunofluorescent staining) ...
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bioRxiv - Plant Biology 2023Quote: ... 1:2500 anti- digoxigenin antibodies fused to the alkaline phosphatase (Roche). Washings were performed twice for 30min in 1x PBST ...
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bioRxiv - Cell Biology 2024Quote: ... 10 minute incubation with biotinylated antibody [anti-HA biotin (Roche 12158167001) diluted 1:100 in wash buffer] ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2h incubation with sheep anti-Digoxigenin-AP (Roche; 1:2000) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The remaining right lungs and spleens of each A/J mouse treated with PBS or BNF 0.0005mg were macerated with protease inhibitor (complete, EDTA-free, Roche Life Science, IN, USA) and centrifuged ...
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bioRxiv - Cell Biology 2023Quote: RNA was obtained from epithelioids 1 week after medium change to mFAD or from mouse esophageal epithelium peeled and incubated with Dispase I (Roche catalog no. 04942086001) diluted at 1 mg/ml in PBS for 15 min ...
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bioRxiv - Pathology 2023Quote: FDB muscles were harvested bilaterally in sterile mouse ringer and maintained overnight in DMEM (supplier) supplemented with collagenase A (Roche, 0.2 mg/ml) and 1% Penicillin- Streptomycin in a CO2 incubator (37°C ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... about 300 to 500 million cells engineered to express the SARS-CoV-2 MGP or NSP13 gene were homogenized in cold NP40 lysis buffer supplemented with protease inhibitor cocktail (Roche, CA, USA). Lysates were cleared by subsequent centrifugation and filtering steps ...
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bioRxiv - Microbiology 2019Quote: Mature cysts were washed in PBS and suspended in lysis buffer (10 mM HEPES, 25 mM KCl, 1 mM CaCl2, 10 mM MgCl2, 2% CHAPS, and 1X Roche protease inhibitor) (Sigma-Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol in MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 300 mM MgAc ...
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bioRxiv - Microbiology 2021Quote: We then targeted the N-(nucleoprotein) gene of SARS-CoV-2 virus with Roche Lightcycler 480 RNA Master Hydrolysis Probes (Roche Basel, Switzerland) with primers developed in house ...
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bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
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bioRxiv - Microbiology 2020Quote: We obtained OP and NP samples from one hospitalised COVID-19 patient who had tested virus-positive three weeks earlier (cobas® SARS-CoV-2 test, Roche diagnostics), from three individuals who had previously tested SARS-CoV-2 positive ...
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bioRxiv - Cancer Biology 2021Quote: ... control and Capecitabine treated tumors were digested for 2 h at 37 °C with a cocktail of Collagenase I (Roche, Ref: 11088793001) and Hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor tissues up to 1 g were mechanically minced and digested in 2 ml RPMI1640 with 200 µg/ml Liberase TL (Roche by MilliporeSigma), 200 µg/ml Liberase DL (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... 0.2 μL of each primer (100mM stock) (Table SI-4) and 5 μL of 2× KAPA SYBR Fast Universal qPCR kit (KAPA Biosystems, USA). Samples were cycled (40 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellets were lysed in 200μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 5 mM MgAc, 2% digitonin, and 1X Roche protease inhibitor cocktail) by incubating on ice for 30 min and then diluted to 1ml with the lysis buffer containing 0.1% digitonin ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in French Press buffer (40 mM HEPES, 100 mM KCl, 20 mM imidazole, 2 mM PMSF, EDTA-free cOmplete protease inhibitor [Roche], pH 7.5) for 1 hour followed by homogenization using tight douncer ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared by scraping cells using 1X lysis buffer (10X recipe-50 mM Tris pH 7.5, Triton 20%, NP40 10%, 2 M NaCl, mixed with cOmplete protease inhibitor tablet - Roche, Product number 11873580001). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged for 10 min at 13,000 rpm 4°C to pellet the cell debris ...
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bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Cell Biology 2022Quote: ... and then homogenized on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol with MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 3 mM MgAc ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in lysis buffer (25 mM HEPES pH 7.5, 400 mM NaCl, 10 mM 2-mercaptoethanol, 0.1% Triton X-100 supplemented with lysozyme and Roche cOmplete protease inhibitor tablet), lysed by sonication (50% duty cycle ...
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Pathology 2021Quote: CCDs were dissected under stereomicroscopic observation either from fresh kidney slices (microperfusion) or after liberase treatment (Blendzyme 2, Roche Diagnostics, Meylan, France) (immunoblotting ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended in Buffer 2 (10 mM Tris-HCl pH=7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 10 min followed by a centrifugation step ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 2 mM MgCl2, 0.3 % Triton X-100, 0.25 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by sonication using Diagenode Bioruptor for 10 min (30 sec ON ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and transferred in an enzyme solution (2 mg/mL Collagenase/Dispase (Roche, Bale, Switzerland), 0,147 µg/mL TLCK (Lonza ...
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bioRxiv - Genetics 2021Quote: ... and cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C followed by a centrifugation step ...
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bioRxiv - Genetics 2021Quote: ... 30 million equivalent cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C and collected cells by a centrifugation step ...
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bioRxiv - Cell Biology 2021Quote: A reference sample was generated by lysing TK6 cells in DPBS with 2% SDS and cOMPLETE protease inhibitors without EDTA (Roche, 1x concentration) at 70 °C ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were resuspended in Lysis Buffer (25 mM HEPES pH 8.0, 250 mM NaCl, 10 mM imidazole pH 8.0, 5 mM 2-mercaptoethanol, 10% glycerol and supplemented with Roche cOmplete protease inhibitor) and sonicated ...
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bioRxiv - Molecular Biology 2021Quote: ... the probe for RNA FISH was prepared with 2 µg of BAC RP183A17 using Nick translation kit (10976776001) purchased from Roche (Basel, Switzerland). Salmon sperm DNA and cot1 DNA were added to the probe to mask the repetitive sequence ...
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bioRxiv - Microbiology 2020Quote: ... was cut into small pieces of approximately 2 mm3 in a petri dish using a sterile scalpel and transferred into a 2 ml MagNA Lyser bead tube (Roche, United Kingdom). Following this ...
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bioRxiv - Genomics 2020Quote: ... The fixed tissue was washed twice for 2 minutes with icecold PBS plus 1x cOmplete™ mini Proteinase Inhibitor (PI) Cocktail (Roche, 11846145001). The tissue was minced by the Dounce Tissue Grinder (Sigma ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The 1st PCR was performed in a 12-μL total volume of reaction mixture containing 6.0 μL of 2 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, MA, USA), 0.72 μL of forward and reverse primer (10 μM) ...
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bioRxiv - Immunology 2022Quote: ... colon tissue was homogenised in 350 µl of lysis buffer (40 ml of PBS, 10%FCS, 2 Complete Protease Cocktail Inhibitor Tablets (Roche, Mannheim, Germany). Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... were homogenized in 2 mL Precellys tube (Bertin Instruments) containing ceramic beads and 600 μL MagNaPure LC RNA Isolation Tissue buffer (Roche Life Science). The homogenate (400 μL ...