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Citations for Roche :
2751 - 2800 of 6945 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole cell lysates were extracted from trans transfected 293T cells or infected LNCaP cells or PC-3 cells by IP lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). An aliquot of the cell lysate was kept as input for western blot analysis ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells were lysed in 1% DMNG buffer (150mM NaCl, 50mM Tris-HCl pH 7.5, 5mM MgCl2, 1% DMNG, protease inhibitors (Roche diagnostics, EDTA free) for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were first thawed on ice and resuspended in 1 mL FA buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate, with Roche cOmplete protease inhibitors (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: ... from 4L of bacterial culture was resuspended in 30 mL lysis buffer (50 mM Tris-HCl pH 8.3, 1 mM EDTA, 150 mM NaCl, 10% glycerol, 5 mM DTT, 1 mM PMSF, and Roche protease inhibitor cocktail) and sonicated on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1, LS005477] and collagenase type B [Roche, 0.35 U·ml-1, 11088807001]). The left ventricle was collected after 10 minutes’ digestion and cut into small pieces ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then incubated for 1 h at 4° C in PBS + 1% BSA with a 1:1000 dilution of rat anti-HA monoclonal antibody (Roche; Basel, Switzerland) and washed 5×10 min on ice with PBS + 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were gently resuspended in 25 ml of swelling solution (1 mM PIPES pH 7.0, 1 mM MgCl2, 5 μg/ml taxol and Roche Complete Protease Inhibitors) and immediately centrifuged at 250 g for 3 min ...
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bioRxiv - Plant Biology 2021Quote: ... Three volumes of lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM KCl, 1% Triton-X100, Protease inhibitor 1 X [Roche], NEM 20 μM) was added to 2 g of powdered tissue ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 mM MgCl2, 1 mm EDTA, 0.5 mM DTT, 50 mM NaF, 50 mM sodium pyrophosphate, 1 mM Na3VO4 and Roche protease inhibitor cocktail) by glass bead lysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected via centrifugation and lysed in lysis buffer (1.5M KCl, 20 mM Tris pH 8.0, 20 mM Imidazole pH 8, 1 mM DTT, 1 tablet of Roche protease inhibitor cocktail). Sonication was performed to further lyse cells and to shear DNA ...
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bioRxiv - Biophysics 2020Quote: ... from 18-day old mouse pups were disrupted using forceps and a razor blade in 1 mL of 1× PBS with protease inhibitors (05896988001, Roche Complete Ultra). The cell suspension was then gently added to a 15-mL conical tube with 5 mL of 1× PBS with protease inhibitors and allowed to settle ...
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bioRxiv - Molecular Biology 2022Quote: ... The adhered cells were then gently washed with cold PBS and lysed in 1% NP-40 lysis buffer (50mM Tris-HCl pH 8.0, 150 mM sodium chloride, 1% NP-40, Roche Complete protease inhibitor). The lysate was then cleared by centrifugation at 17,000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2% NP-40, 10% glycerol, 1 mM EDTA, 1 mM PMSF, 20 µM MG132, 5 mM DTT and Roche protease inhibitor #5892953001), and incubated for 1 h on a rotating wheel ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor).
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche, 11175041910] in Maleic acid) for 1 h ...
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bioRxiv - Genetics 2019Quote: ... resuspended in 60 ml hypotonic lysis buffer (20 mM HEPES-KOH pH 7.5, 5 mM NaCl, 1 mM MgCl2, 1 mM PMSF and EDTA free protease inhibitor mixture from Roche and Thermo Scientific) and incubated for 15 min on ice ...
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bioRxiv - Immunology 2019Quote: ... and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS, 1% Triton X-100, 2mM EDTA, 50mM Hepes-KOH [pH 7.5], 0.1% sodium deoxycholate, Roche 1X Complete Protease Inhibitor). Nuclei were lysed for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM MgOAc, 15 mM Tris-HCl pH 7.4, 0.5% NP40, 1 mM DTT, 1 μl RNAsin, Roche protease inhibitors without EDTA). The cells were lysed by one freeze-thaw cycle in large volume (15–50 ml ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining cell pellet was weighed and resuspended in a 1:1 ratio with ice-cold 1x PBS with a protease inhibitor tablet (Roche Life Sciences) and 1mM PMSF ...
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bioRxiv - Physiology 2021Quote: ... 1:500 goat anti-rabbit (AlexaFlour 488; A-11008) and 1:1000 4′,6-diamidino-2-phenylindole (DAPI; Roche 10236276001; Basel, Switzerland). After secondary incubations ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... The cells were lysed using RIPA buffer (Tris-HCl 10 mM, 1% SDS, 10 mg/ml deoxycholate, 150 mM NaCl, 1% NP40 and protease inhibitors cocktail [Roche, Mannheim, Germany]). The total protein samples were separated using by 12% SDS-PAGE and transferred onto nitrocellulose membranes (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM β-glycerophosphate and 1% Triton) containing protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Five hunderd mL Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% SDS, 1 mM PMSF, and EDTA-free protease inhibitor cocktail from Roche, dissolved in PBS) was added and cells were harvested from the Transwell membrane using a disposable cell scrapper and transferred to an Eppendorf tube ...
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bioRxiv - Synthetic Biology 2022Quote: ... 140 mM NaCl, 1mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.1% Na-Deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 200 µL Roche cOmplete protease inhibitors). 0.5 mm diameter glass beads were added to 1 mm below the meniscus ...
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bioRxiv - Neuroscience 2022Quote: Cells were homogenized in 1 % NP40 lysis buffer (20 mM HEPES pH7.5, 150 mM NaCl, 1 % NP-40, 10 % Glycerol and Roche protease inhibitor mix) on ice ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sodium deoxycholate, 50 mM NaF, 2 mM EDTA, 2 mM DTT, 0.2 mM Na orthovanadate, 1 X Roche protease inhibitor #11836170001). Lysates were sonicated and centrifuged to remove debris ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... tubes and homogenized for 1.5 minutes in 50μL of TBS containing 1% Triton X-100 (Table 1) and 1X protease and 1X phosphatase inhibitor cocktails (Roche, 04693124001 and 04906837001). The homogenates were then probe-sonicated with a Branson Sonifier® SFX150 (Emerson ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (Retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor). Pellets were either processed immediately or flash-frozen in liquid nitrogen and stored at -80°C ...
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bioRxiv - Cell Biology 2024Quote: ... the full-length Treacle was amplified by PCR from cDNA with primer set #1 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained XhoI sites ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 1x cOmplete protease inhibitor cocktail [Roche-11697498001], 1 mM PMSF), incubated on ice for 10 minutes and centrifuged for 10 minutes at 21100 x g at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei pellets were resuspended in 100 µl of nuclei lysis buffer (20 mM HEPES pH 7.9, 0.1 M NaCl, 1mM EDTA, 1 mM EGTA, 1mM DTT, 25 % glycerol, 0.5 mM AEBSF, 1×Roche complete protease inhibitor cocktail) for 20 min at 4 °C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 0.1 mM EGTA, 100 mM NaCl, 1% Nonidet P-40, 10 mM Imidazole, supplemented with Roche protease inhibitor cocktail). Cell lysates were clarified by centrifugation at 10,000× g for 5 min at 4 °C and then incubated at 4 °C under constant rotation with His-ΔFIP1c-RCP559-649 fusion protein ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was extracted using NETN buffer (20 mM Tris-HCl pH 8, 420 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1 mM DTT, and Roche Protease Inhibitor Cocktail) and were resolved in 8% Bis-Tris SDS-PAGE gels ...
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bioRxiv - Microbiology 2023Quote: ... Cells were collected and lysed in lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Diagnostics, Mannheim, Germany]) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8 and 10 mM NaF completed with 1 × protease inhibitor cocktail (Pierce, #A32955) and 1 × phosphatase inhibitor cocktail (Roche PhosSTOP, #490684001). The total protein concentration was determined using Bradford assay (Protein Assay Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.5% NP-40 [Thermo Scientific, Waltham, MA, USA], EDTA-free protease inhibitor [Roche, Basel, Switzerland]). Lysed astrocytes were collected into 1.5 mL microcentrifuge tube ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... to prepare for an hour long blocking in 10% lamb serum/TBST and an overnight incubation at 4°C in a 1% lamb serum containing Anti-Dig-AP antibody (Roche, 1:5000). The next morning ...
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bioRxiv - Biophysics 2021Quote: ... Primary antibodies were: rat-anti-HA (1:5,000; Roche Applied Science catalog ...
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bioRxiv - Cancer Biology 2021Quote: ... 5mM EDTA) with 1× cOmplete protease inhibitors (Roche 11697498001), incubated on ice for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche), after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman) ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-GFP (Roche, 11814460 001, 1:2000), monoclonal mouse anti V5 (Invitrogen R960-25 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM NaVO3 and a protease inhibitor tablet (Roche)) ...