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Citations for Roche :
2701 - 2750 of 3754 citations for Mouse Anti Dengue Virus Membrane Protein Serotype 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Lyzed cells were centrifuged at 20,000 x g for 30 min at 4°C and the supernatant was mixed with protease inhibitor (cOmplete ULTRA Tablets, Roche, Sigma-Aldrich, Cat: 05892953001). Protein concentration was determined using the Qubit Protein Assay Kit and Fluorometer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... expanded leaves of 7-week old hybrid aspen plants were sampled with a biopsy punch (4 mm diameter) and loaded into wells of 96-well plate (Roche LightCycler® 480 System) containing 100 μL of water (one sample per well) ...
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bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded in conditioned medium in triplicate in 6 cm plates and allowed to attach for 4 h before treatment with mitomycin C (Roche 10 107 409 001) or olaparib (Selleckchem AZD2281 ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS containing 0.5% Tween-20 samples were incubated with fluorescent labelled secondary antibodies containing 4′,6-diamidino-2-phenylindole (DAPI; Roche Cat# 10236276001, 1.0 µg/ml) for 30 min ...
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The HEK 293T cells were mixed with 1 μg of mouse Ano9 cDNA (mAno9-pEGFP-N1) and 3 μl FuGene HD (Roche Diagnostics). The transfected cells were plated onto glass coverslips that were kept in a 35-mm round Petri dish ...
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bioRxiv - Systems Biology 2020Quote: ... We generated digoxigenin (DIG)-labeled mRNA antisense probes against Nrxn3 using reverse-transcribed mouse cDNA as a template and an RNA DIG-labeling kit from Roche (Sigma). Primer and probe sequences for the Nrxn3 probe is available in Allen Brain Atlas (http://www.brain-map.org) ...
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bioRxiv - Developmental Biology 2020Quote: PCR was performed on genomic DNA extracted from cell pellets or mouse tails to determine the loss of the Y chromosomal genes Uba1y and Ssty1 by utilizing KAPA Mouse Genotyping Kit (Roche, KK7302). Specific procedures were followed according to the reagent instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the tail of each embryo was collected for genomic DNA extraction using the KAPA Mouse Genotyping Kit HotStart (Kapa Biosystems, KK7352). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... 100 ng of total RNA from each sample was incubated with RiboErase Mouse rRNA depletion oligos per the manufacturer’s instructions using Kapa RiboErase (HMR) Module (Kapa Biosystems, USA). Resulting rRNA duplexed with DNA oligos were digested with RNase H treatment ...
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bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled mRNA against Dbh using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Slc17a7 (Vglut1) ...
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bioRxiv - Neuroscience 2024Quote: ... flash frozen mouse brains were thawed in 0.32M sucrose supplemented with protease inhibitor cocktail tablets (cOmplete Mini EDTA-free; Roche, Mannheim, Germany). Thawed brains were next manually homogenized using a pestle and 16G/25G needle ...
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bioRxiv - Cancer Biology 2020Quote: ... protein lysates were prepared from HCC1428 cells with control or E2-E10 siRNA knockdown using modified Tissue Protein Extraction Reagent (TPER) (Pierce) and a cocktail of protease and phosphatase inhibitors (Roche Life Science). The protein lysates were then diluted into 0.5 mg/ml of total protein in SDS sample buffer and denatured on the same day ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Cell Biology 2021Quote: ... the pellet washed in 1x PBS then re-suspended in 1x protein loading buffer (4x NuPAGE® LDS sample buffer [Invitrogen], 1x PBS and 25 uL ß-mercaptoethanol, 2x Roche cOmplete Mini protease inhibitor cocktail tablets ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... The gene encoding this protein was amplified from genomic DNA of the SECphi4 phage using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601) with primers AS330+AS331 (Table S6) ...
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bioRxiv - Cancer Biology 2022Quote: Total protein cell extracts were obtained by lysing the cells with RIPA buffer supplemented with 1x protease inhibitors (PI) (Roche, Milan, Italy). Proteins were quantified using the BCA method (Pierce ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were prepared using T-PER (Pierce Chemical Co., IL) in the presence of protease inhibitors (Roche Applied Science, Barcelona, Spain), and subjected to Western blot analyses with anti-rat anti-ABCA1 antiserum (kindly donated by Dr ...
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bioRxiv - Molecular Biology 2022Quote: Mouse liver tissues and cells were homogenized and lysed in Protein Extraction Reagent (P0013, Beyotime Biotechnology) supplemented with protease inhibitor cocktail (04693159001, Roche, Basel, Switzerland). Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 HUVEC were washed with CUT&RUN wash buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 500 nM spermidine, 1X Roche Protein Inhibitor Cocktail) at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and incubated for 12 h with addition of alkaline phosphatase coupled with anti-digoxigenin antibody for amha and anti-Fluorescein antibody for amhby (1:2000, Roche Diagnostics Corp, Indianapolis, IN). Samples were then washed with PBS solution ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-Digoxigenin-AP Fab fragments (Roche Applied Science, Indianapolis, IN) was incubated in antibody block overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... anti-digoxigenin sheep polyclonal (11 333 089 001, Roche, Switzerland), and anti-FLAG mouse monoclonal (M185-A48 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with anti-DIG HRP antibody (Roche), and the signals for egr1 were detected with TSA Cy3 kits (PerkinElmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated with sheep-anti-DIG (1/1000, Roche) followed by biotinylated donkey-anti-sheep (1/500 ...
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bioRxiv - Neuroscience 2021Quote: ... 1:500 anti-fluorescein-POD (Roche (11 426 346 910)) 1/2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... an anti-HA-tag antibody (#11867431001) was purchased from Roche, an anti-Flag-tag antibody (#012-22384 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 µg/mL anti-His6 (Roche #11 922 416 001). Total protein on lysate membranes was stained with 50% methanol with 0.1% Coomassie Brilliant Blue R-250 dye ...
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bioRxiv - Genomics 2020Quote: ... GFP was detected using an anti-GFP antibody (11814460001, Roche), a peroxidase-conjugated second antibody (414151F ...
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bioRxiv - Genomics 2020Quote: ... and rhodamine-conjugated anti-digoxigenin for dig-labeled probe (Roche). The DNP-labeled probe was detected with rabbit anti-DNP ...
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bioRxiv - Developmental Biology 2019Quote: ... Probes were detected with anti-DIG-AP (1:5000, Roche) or anti-FLU-AP (1:10000 ...
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bioRxiv - Microbiology 2019Quote: Antibody concentrations used were: rat anti-HA high affinity (Roche) (1:1000) ...
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bioRxiv - Cell Biology 2019Quote: ... was incubated with 1 μg anti-HA (Roche, clone 3F10) for at least 4 hr at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... sheep anti-Digoxigenin conjugated with alkaline phosphatase (1:2000; Roche), mouse antiEven-Skipped (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-HA-tag mAb (clone 3F10, Roche, no. 11867423001), rabbit anti-SEC61A mAb (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with anti-DIG antibody conjugated with alkaline phosphatase (Roche) diluted 1:2000 in TBS with 1% BSA and 0.05% Triton X-100 for 12 hr at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... living neurons were incubated with the anti-HA antibody (Roche) 1 μg/ml in the electrophysiology external recording solution for 4 minutes and subsequently with the diluted QDs solution for 3 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... rat anti-HA mAb clone 3F10 (Roche, 118673230002, 1:100), and chicken anti-GFP pAb (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... rat monoclonal anti-HA (1:800, clone 3F10 Roche/Sigma).
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bioRxiv - Neuroscience 2020Quote: ... and rat anti-HA (rat mab 3F10; Roche, 1:200) antibodies diluted in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-fluorescein/DIG-Alkaline phosphatase (AP) (1:1000; Roche) antibodies were used ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary antibodies used were rat anti-HA (clone 3F10, Roche) at a dilution of 1:3000 for blots exposed to film ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with AP-conjugated anti-DIG antibody (Roche Diagnostics). A colorimetric NBT/BCIP (Roche Diagnostics ...