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Citations for Roche :
2701 - 2750 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The membranes were probed overnight at 4°C with monoclonal anti-GFP primary antibodies (Roche, 11814460001) at 1:1,000 and then with anti-mouse peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... and nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrate (Roche Diagnostics). The color was allowed to develop for 5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT) substrate (Roche Diagnostics) for chromogenic detection ...
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bioRxiv - Cell Biology 2024Quote: ... human skin was cut into thin pieces and incubated overnight at 4°C with dispase (Roche) at 2.4 U/ml ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015, 5mg/ml dissolved in Basis medium, DNase: 15ug/ml (Roche, 10104159001) and 10 μM Y-27632-HCl Rock inhibitor (Selleckchem ...
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bioRxiv - Neuroscience 2022Quote: ... 93070) using a syringe plunger with 20 mL of 0.01M PBS and 0.01 mg/mL of papain (Roche, cat no. 10108014001), 0.05% collagenase (Roche ...
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bioRxiv - Microbiology 2022Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Immunology 2022Quote: ... We transferred the minced tissue to a tube containing 5 mL of digestion Buffer containing collagenase D (2mg/mL, Roche #11088858001) and DNase (0.125 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015, 5mg/ml dissolved in Basis medium, DNase: 15ug/ml (Roche, 10104159001) and 10 μM Y-27632-HCl Rock inhibitor (Selleckchem ...
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bioRxiv - Molecular Biology 2021Quote: ... treated with 5.8 μl of proteinase K (24 mg/mL, P4850) for 45 min at 55°C followed by 50 μg/ml RNase A (Roche, 10109169001) for 1 h at 37°C plus 1 h at 65°C ...
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bioRxiv - Immunology 2022Quote: Skin biopsies were collected and minced with sterile scissors before digestion in 1 mL of DMEM with 1 mg/mL collagenase D (Roche Diagnostics) for 3 hours at 37℃ ...
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bioRxiv - Immunology 2022Quote: ... We transferred the minced tissue to a tube containing 5 ml of digestion buffer containing collagenase D (2mg/ml, Roche #11088858001) and DNase (0.125 mg/ml ...
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bioRxiv - Microbiology 2022Quote: Single cell suspensions from the ear were obtained by using tweezers to separate the dorsal and ventral sides of the mouse ear and placing the sheets dorsal-side down in a 24 well plate with 1 mL/well of RPMI1640 containing 0.25 mg/mL of Liberase TL (Roche, Diagnostics Corp.) and 10 μg/mL of DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... threshold values for biomarker aggregation also differ (amyloid-β: 192 pg/mL for INNO-BIA AlzBio3, 980 pg/mL for Roche Elecsys ...
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bioRxiv - Cell Biology 2024Quote: ... deparaffinized microsections were rehydrated and incubated with hyaluronidase (4mg/mL in PBS, pH 5.5) followed by pronase (1mg/mL in PBS, pH 7.4, both Roche Diagnostics, Mannheim, Germany). After blocking with 5% bovine serum albumin sections were incubated with a mouse anti-human type II collagen antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... cut into small pieces with a scalpel and transferred into 1 ml collagenase-dispase solution (1 mg/ml in PBS, Roche #10269638001) for 10 min at 28°C ...
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bioRxiv - Neuroscience 2023Quote: ... Expi293F cell pellets (from 50 ml of culture) were thawed and resuspended in 8 ml of 0.25 x PBS with protease inhibitors (Roche, Indianapolis, USA). Cells were lysed using a Dounce homogenizer on ice and then centrifuged in a Beckman SW55 rotor at 100,000xg for 45 min ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant baculoviruses were generated by transfecting 2.5 μg of a transfer bacmid into Sf9 cells (2.5 mL at a density of 106 cells/mL) using 3 μL of X-tremeGENE™ HP DNA Transfection Reagent (Roche) and 100 μL Transfection Medium (Expression Systems) ...
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bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μl papain inhibitor solution containing 5 mg/ml BSA (Carl Roth, 8076.4) and 5 mg/ml Trypsin inhibitor (Sigma-Aldrich/Roche, 10109878001) in PBS was added ...
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bioRxiv - Plant Biology 2021Quote: ... Twenty-ng cDNA was used for each qPCR reaction with the KAPA SYBR FAST qPCR Master Mix (2X) (KAPA Biosystems, Wilmington, MA, United States) and a real-time PCR system (Applied Biosystems QuantStudio 3) ...
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bioRxiv - Cell Biology 2020Quote: ... For the qPCR 20 ng of cDNA were used per reaction and amplified with SybrGreen I master mix from Roche (04 887 352 001) at 60 °C and values were normalized to GAPDH ...
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bioRxiv - Developmental Biology 2021Quote: ... Resultant templates were gel extracted then 500 ng transcribed into RNA using T7 RNA polymerase (ThermoScientific, EP0111) and either dig- or FITC-labelling mix (Roche, 3935420 and 32874620 respectively). RNA probes were purified using Microspin G25 (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... a final concentration of 1 ng/μl cDNA was mixed with 2 μl FastStart DNA Master SYBR Green I (Roche #03 003 230 001), 0.5 μM forward and reverse primer ...
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bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1% Triton X-100) with cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche, cat# 11873580001), 1 mM phenylmethylsµlfonyl fluoride (PMSF) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 mM EDTA and 0.1% Triton X-100) supplemented with proteinase inhibitor mixture (cOmpleteTM; Roche), and incubated with 5 μg of histones in 250 μl of reaction buffer at 4°C for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Captured peptides were then eluted from thiopropyl sepharose by incubation with 100 mM DTT (Roche) in 50 mM NH4HCO3 at 70°C for 45 minutes and further processed for mass spectrometry sequencing at the University of Cincinnati Proteomics Laboratory 67 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.4 (Buffer A) with 0.5% Triton X-100 and Complete anti-protease (Roche, 11245200) was added to each plate ...
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bioRxiv - Biochemistry 2019Quote: ... then protein was eluted with 100 µM imidazole in the presence of protease inhibitors (Roche) and desalted using a PD-10 column ...
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bioRxiv - Cell Biology 2019Quote: ... The antibodies used for IFA were: rat anti-HA antibody (clone 3F10; Roche, 1:100), mouse anti-AMA1 (1F9 from Alan Cowman) ...
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bioRxiv - Cell Biology 2020Quote: ... 0,5% Triton X-100) supplemented with Complete Mini EDTA-free Protease inhibitor cocktail (Roche, 04693159001) and PhosSTOP (Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 in PBS containing protease inhibitor cocktail (cOmplete™, EDTA-free, Roche) was added to the cleared homogenates to make total testis lysate ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.1% Triton X-100) supplemented with 1x EDTA-free protease inhibitor cocktail (Roche, 04693159001), 1 μg/mL Pepstatin A (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1% Triton X-100) with cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche, cat# 11873580001), 1 mM phenylmethylsεlfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2021Quote: ... 5% glycerol) containing 100 mM Phenylmethylsulphonylfluoride (PMSF) and EDTA-free Protease inhibitor cocktail tablets (Roche). Resuspended cells were first treated with 1 mg/ml Lysozyme to digest the cell wall and subsequently frozen at −80°C overnight ...
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bioRxiv - Plant Biology 2020Quote: ... 2% Triton X-100 and cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland). Samples were heated at 70 °C for 15 min with 1 x Bolt LDS sample buffer (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Signals from DIG-riboprobes were detected with peroxidase-conjugated anti-DIG antibody (1:100, Roche), amplified using TSA Plus DIG kit (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... lysed by triturating in PBS containing 0.05% Triton X-100 and protease inhibitors (cOmplete, Roche) and clarified by 5 min sequential centrifugations at 500xg and 1000xg ...
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bioRxiv - Neuroscience 2020Quote: ... each slide was incubated with 100 µL antidioxigenin alkaline phosphatase-conjugated antibody (Roche, Mannheim, Germany) diluted 1:500 in 1% blocking reagent in TBS plus 0.03% Triton-X100 at 37 °C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 (Buffer A) with 0.5% Triton X-100 and Complete anti-protease (Roche, 11245200). Cell homogenates were incubated on ice for 30 minutes and centrifuged at 16,100 g for 10 min and the clarified supernatant was recovered ...
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β-amyloid−driven synaptic depression requires PDZ protein interaction at AMPA-receptor subunit GluA3bioRxiv - Neuroscience 2021Quote: ... 150 mM NaCl) containing 2% Triton X-100 and EDTA-free protease inhibitor cocktail (Roche). The resulting samples were incubated for 1 h at 4°C and spun down twice at 20,800 x g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% SDS and 1% Triton X-100) supplemented with phosphatase inhibitors (cOmplete tablets, Roche Diagnostics) or according to the REAP protocol 44 ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% SDS and 1% Triton X-100) supplemented with phosphatase inhibitors (cOmplete tablets, Roche Diagnostics)] ...
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bioRxiv - Microbiology 2021Quote: ... RNase activity was inactivated by adding 100 unites of Protector RNase Inhibitor (Roche, Basel, Switzerland). Total RNA (including RNA from approximately 18 nucleotides or more ...