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Citations for Roche :
2651 - 2700 of 6260 citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability assays were conducted using WST-1 reagent (Roche). Tissue wares were purchased from BioStar ...
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bioRxiv - Genomics 2024Quote: ... and 1 Complete EDTA-free Protease Inhibitor cocktail Tablet (Roche)/50 mL buffer) ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% Nonidet-P40, 0.5% Deoxycholate, 0.1% SDS, complete Protease Inhibitor Cocktail [Roche, Palo Alto, CA], and 500 μM PMSF) and centrifuged for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... were collected from CX3CR1GFP/+ mice and incubated isolated in 1 ml of RPMI medium containing 1mg/ml of collagenase D (Roche, cat. no. 1-088-874) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Probes were detected by horseradish peroxidase (POD)-conjugated antibodies (anti-FITC-POD at 1/250 dilution, Roche; anti-DIG-POD at 1/500 dilution, Roche). Signals were amplified by biotin-conjugated tyramide (PerkinElmer ...
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bioRxiv - Neuroscience 2021Quote: ... 300 mM NaCl, 30 mM imidazole, 1 M urea, 1% v/v Triton X-100, 5 mM beta-mercaptoethanol, with Roche EDTA-free cOmplete protease inhibitor) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Genomics 2023Quote: ... The brain samples were homogenized in RIPA with 1% SDS and protease inhibitor cocktail (1 tablet per 10 ml, Roche complete, EDTA-free, Sigma-Aldrich) using a sonicator with a 10 s pulse at 40% amplitude.
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were extracted by mixing 500 mg of ground tissue with 2.0 ml of extraction buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, cOmplete Mini EDTA-free Protease Inhibitor Cocktail [Roche, 1 tablet per 10 ml]), followed by brief sonication in an ultrasonic bath for 5 s ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA) supplemented with phosphatase inhibitors (5 mM ß-glycerophosphate, 5 mM NaF, 1 mM Na3VO4) and protease inhibitors (Roche cOmplete ULTRA Tablets, EDTA-free). The lysates were sonicated on ice (4x 10s bursts ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM MgCl2, 1% Triton X-100, 50 mM HEPES pH 7.4, 1 x Protease Inhibitor Cocktail [04693159001, Roche Diagnostics], 50 mM NaF, 0.2 mM Na3VO4). The protein concentration was determined by BCA assay (23225 ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 2 mM MgCl2, 1% Triton X-100, 50 mM HEPES pH 7.4, 1 × Protease Inhibitor Cocktail [04693159001, Roche Diagnostics], 50 mM NaF, 0.2 mM Na3VO4). The remaining (87% ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was assessed using colorimetric WST-1 reagent (Roche, 11644807001) as per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... and 1× protease inhibitor cocktail (Catalog No. 11697498001, Roche, Basel, Switzerland). Lysates were centrifuged (20,000 g ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-HA (1:1000) (Clone 3F10; Roche Diagnostics, Indianapolis, IN) and mouse anti-α-Tubulin (1:10,000 ...
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bioRxiv - Cell Biology 2019Quote: ... 1.8 mM CaCl2 and 1 mM MgCl2) containing 0.1% collagenase (Roche) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM dithiothreitol and 1x complete mini protease cocktail (Roche, Switzerland)) ...
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bioRxiv - Developmental Biology 2021Quote: ... and Rat anti-HA (1:500; Roche, 11 867 423 001). The following secondary antibodies were used ...
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bioRxiv - Microbiology 2021Quote: ... 1 mg/ml lysozyme) supplemented with complete protease inhibitor cocktail (Roche), incubated for 1 hour on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 tablet per 10 mL of cOmplete tablets EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... and 1 mM DTT) with a protease inhibitor cocktail tablet (Roche). The lysate was spun at 160,000x g for 2 h ...
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Resurgent Na+ currents promote ultrafast spiking in projection neurons that drive fine motor controlbioRxiv - Neuroscience 2021Quote: ... incubated with alkaline phosphatase conjugated anti-DIG antibody (Roche, 1:600), and developed overnight in BCIP/NBT chromagen (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibody were rat anti-HA (MAb 3F10 1:20, Roche) or rabbit anti-FLAG (M2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% Triton X-100) supplemented with protease inhibitor cocktail (Roche, 11697498001). The supernatant was collected after centrifugation at 13000 RPM at 4°C for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% Triton X-100) supplemented with cOmplete protease inhibitor cocktail (Roche). DNA was sheared in polystyrene tubes in a Q700 cup sonicator (Qsonica ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF supplemented with protease inhibitor cocktail (Roche, Cat #118575). Protein lysate was quantitated using Pierce BCA protein assay kit ...
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bioRxiv - Cell Biology 2022Quote: Cells were stained with antibodies against GFP (Roche; 1181446001; 1:1000), GFP (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL 1M DTT and 1 tablet of protease inhibitor (Roche). The lysate was sonicated and then centrifuged at 48,000 x g for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and a complete protease inhibitor tablet (Roche; 1 tablet/10 mL)) for 10 min with gentle rocking ...
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bioRxiv - Microbiology 2020Quote: ... and a complete protease inhibitor tablet (Roche; 1 tablet/10 mL)) for 5 minutes per wash ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-GFP clones 7.1 and 13.1 (Roche 11814460001, 1:500), rabbit anti-Cpn60 (From ...
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bioRxiv - Physiology 2019Quote: ... dissociated with 1 μg/mL collagenase/dispase (Roche Life Science, Germany) in neurobasal medium for 90 minutes at 37°C on a rotator-shaker ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) containing a protease inhibitor cocktail (cOmplete, Roche) and a protein phosphatase inhibitor tablet (PhosSTOP ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT] containing a protease inhibitor cocktail (cOmplete, Roche) and a protein phosphatase inhibitor tablet (PhosSTOP ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight incubation in anti-DIG antibody solution (1/2000; Roche #11093274910) at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... all samples contained 1× protease inhibitor cocktail (cOmplete EDTA free, Roche) and 1× phosphatase inhibitor cocktail (PhosSTOP ...
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bioRxiv - Immunology 2019Quote: ... and subjected to 1 hour treatment with bacterial sialidase C (Roche) at 37C ...
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bioRxiv - Genomics 2019Quote: ... EDTA-free protease inhibitors (1 tablet for 50 ml buffer; Roche), 5 mM DTT).
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bioRxiv - Genomics 2019Quote: ... EDTA-free protease inhibitors (1 tablet for 50 ml buffer; Roche), 5 mM DTT ...
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bioRxiv - Developmental Biology 2019Quote: ... 1% Triton X-100] supplemented with protease and phosphatase inhibitors (Roche). 10mg of protein from each sample was resolved by 4-15% SDS-polyacrylamide gel electrophoresis and transferred to the nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2019Quote: ... by using peroxidase-conjugated primary antibodies against fluorescein (1:250, Roche) and digoxigenin (1:50 ...
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bioRxiv - Immunology 2019Quote: ... 1% Triton-X-100) including proteinase and phosphatase inhibitors (1x Roche complete mini pill ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and protease mini EDTA-free inhibitor cocktail (Roche). Cells were incubated for 30 min on ice and lysed by passing through a 21-gauge needle 10 times ...
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bioRxiv - Developmental Biology 2019Quote: ... Slides were then incubated with Anti-Dig-Peroxidase (Roche, 1:500), rat anti-Endomucin (1:200) ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were used: sheep anti-Digoxigenin (1:2000; Roche), sheep anti-Digoxigenin conjugated with alkaline phosphatase (1:2000 ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 1 tablet of Complete mini EDTA-free mixture (Roche) and 1 tablet of PhosSTOP phosphatase inhibitor cocktail mixture (Roche ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... in the presence of Biotin-16-UTP at 1 mM (Roche), as previously described (26) ...
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bioRxiv - Biochemistry 2019Quote: ... filtered and loaded onto a Ni2+ -chromatography column (1 ml, Roche), washed in 20 CV buffer (50 mM Tris ...