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Citations for Roche :
2551 - 2600 of 3381 citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and qPCR was carried out in 5 µL multiplexed reactions and 384-well format using the LightCycler 480 Instrument II (Roche). Final concentration of primers are 0.5 µM and probes are 0.25 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 µl of diluted cDNA was used for each qPCR reaction carried out using KAPA SYBR FAST master mix (Roche). Relative expression levels for each gene were determined based on the 2−ΔΔCT method normalizing against 7SK RNA.
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bioRxiv - Plant Biology 2024Quote: The pulverized tissue was incubated in 30 mL of buffer 1 (400 mM sucrose, 10 mM Tris-HCl pH 8.0, 10 mM MgCl2, 5 mM β-mercaptoethanol, 1 mM PMSF, 1× Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 1 mM ATP, 1% IGEPAL, 5% glycerol, 3 U/ml Benzonase and Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an 8-minute perfusion with myocyte digestion buffer containing 5 mg of liberase dispase high DH enzyme (Roche) per digestion ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Molecular Biology 2023Quote: ... Four hippocampi and 4 cortices from 8-month-old 5xFAD mice were homogenized in ice cold sucrose buffer (320 mM sucrose, 5 mM Hepes pH 7.4) supplemented with protease (Roche #05056489001) and phosphatase inhibitors (Invitrogen #A32957 ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... frozen tissue fragments were thawed and incubated for 5 minutes at 37°C in a dissociation buffer containing RPMI-1640 supplemented with 10 U/mL DNase (Roche), 200 U/mL collagenase IV (Worthington Biochemical) ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were resuspended in binding buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 5 mM TCEP) supplemented with protease inhibitors (Roche cOmplete), sonicated ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by sonication and French Press in purification buffer (50mM sodium phosphate buffer pH 7, 300 mM NaCl) supplemented with 5 ug/mL DNaseI (Roche), 5ug/mL RNAse (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 8, 450 mM NaCl, 1% CHAPS, 20 mM MgCl2, 5 mM ATP, 1 mM Dithiothreitol [DTT], 1X Roche Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cell Biology 2023Quote: ... Two brains were transferred to a pre-chilled 30-mL glass tube Wheaton homogenizer containing 5 mL of cold ‘solubilisation-plus’ buffer (solubilisation buffer with 1X COMPLETE protease inhibitor and 1X PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with 1x PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1x PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Genomics 2023Quote: ... Using a P7-specific primer (Extended Data Table S4) UMI’s were added to cDNA (in 5 reactions) with Kapa 2x HiFi HotStart ReadyMix (Kapa Biosystems). Reactions were pooled and purified ...
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bioRxiv - Biochemistry 2023Quote: ... and the pellet was resuspended in 5 volumes of the lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche); buffer A ...
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bioRxiv - Microbiology 2022Quote: ... The total qPCR reaction volume was 1.5 □μL with 0.5□ μl of DNA (40□ng□μl-1) and 1□μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5□μM of PCR primers ...
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bioRxiv - Cancer Biology 2023Quote: Tissues from animals were homogenized and lysed in HED buffer (25 mM HEPES, 5 mM EDTA, 10% glycerol, 1 mM DTT, and 1x cOmplete protease inhibitor [Roche]). Lysates were sonicated for 20 minutes in a water bath sonicator and cleared by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.0, incubated in a hybridization buffer [(50% formamide, 5× SSPE, 0.1% SDS, and 1 mg/ml Yeast tRNA (Roche Diagnostics)] containing the Avidin solution (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of sample were used in a reaction mix containing 1 μL 10 μM of each primer (0.5 μM final) and 5 μL 2x Light Cycler SYBR Green LightCycler® 480 SYBR Green I Master (Roche). For telomere amplification ...
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 0.1 % Triton X-100, complete protease inhibitor cocktail [Roche] added fresh) for 10 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting cDNAs were amplified with primers DP3 and DP5 (5’-GTTCAGAGTTCTACAGTCCGACGATC-3’, 0.5 μM) and KAPA Hifi HotStart DNA polymerase (Roche, KK2601) to the optimal amplification point ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.44 M sucrose, 1.25 % Ficoll, 2.5% Dextran, 10 mM MgCl2, 0.5% Triton X-100, 5 mM DTT, 1x Roche protease inhibitor mixture), filtered through two layers of Miracloth ...
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bioRxiv - Immunology 2023Quote: Whole lung single cell suspensions were prepared by harvesting lung lobes into 5 ml HBSS with 40µl Liberase Tm (0.1 U/ml, Roche, Cat# 5401127001) and 20µl DNAse 1 (10mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Biochemistry 2023Quote: ... 100 mM NaCl, 20 mM Imidazole pH 7.5, 3 mM MgCl2, 100 µM EDTA, 5 mM β-Mercapoethanol, 20 µM GDP, Roche cOmplete protease inhibitor cocktail and DNAse I ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Genetics 2024Quote: ... Blots were then exposed to 2.5 pmol of 40-nt HPLC purified DNA oligonucleotides conjugated to digoxigenin (DIG) using the DIG Oligonucleotide Tailing Kit (Roche) hybridized to the nitrocellulose membrane at 60°C overnight (42°C for 2 h for 5S rRNA ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed using the first-strand cDNAs diluted 5-fold in water and KAPA SYBR FAST qPCR Master Mix (2x) kit (Roche) and gene-specific primers in a LightCycler 96 (Roche).
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were serially applied using the U DISCOVERY 5-Plex IF procedure and the following reagents and kits (all from Ventana Medical Systems, Roche): DISCOVERY inhibitor (760-4840) ...
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...