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Citations for Roche :
2501 - 2550 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected via centrifugation and lysed in lysis buffer (1.5M KCl, 20 mM Tris pH 8.0, 20 mM Imidazole pH 8, 1 mM DTT, 1 tablet of Roche protease inhibitor cocktail). Sonication was performed to further lyse cells and to shear DNA ...
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bioRxiv - Biophysics 2020Quote: ... from 18-day old mouse pups were disrupted using forceps and a razor blade in 1 mL of 1× PBS with protease inhibitors (05896988001, Roche Complete Ultra). The cell suspension was then gently added to a 15-mL conical tube with 5 mL of 1× PBS with protease inhibitors and allowed to settle ...
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bioRxiv - Molecular Biology 2022Quote: ... The adhered cells were then gently washed with cold PBS and lysed in 1% NP-40 lysis buffer (50mM Tris-HCl pH 8.0, 150 mM sodium chloride, 1% NP-40, Roche Complete protease inhibitor). The lysate was then cleared by centrifugation at 17,000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor).
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche, 11175041910] in Maleic acid) for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM MgOAc, 15 mM Tris-HCl pH 7.4, 0.5% NP40, 1 mM DTT, 1 μl RNAsin, Roche protease inhibitors without EDTA). The cells were lysed by one freeze-thaw cycle in large volume (15–50 ml ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining cell pellet was weighed and resuspended in a 1:1 ratio with ice-cold 1x PBS with a protease inhibitor tablet (Roche Life Sciences) and 1mM PMSF ...
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bioRxiv - Immunology 2020Quote: ... cut into small pieces and digested for another 1 h at 37°C in 1 mg/ml Collagenase D (Roche, Basel, Switzerland). To obtain single-cell suspensions ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... The cells were lysed using RIPA buffer (Tris-HCl 10 mM, 1% SDS, 10 mg/ml deoxycholate, 150 mM NaCl, 1% NP40 and protease inhibitors cocktail [Roche, Mannheim, Germany]). The total protein samples were separated using by 12% SDS-PAGE and transferred onto nitrocellulose membranes (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM β-glycerophosphate and 1% Triton) containing protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Five hunderd mL Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% SDS, 1 mM PMSF, and EDTA-free protease inhibitor cocktail from Roche, dissolved in PBS) was added and cells were harvested from the Transwell membrane using a disposable cell scrapper and transferred to an Eppendorf tube ...
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bioRxiv - Synthetic Biology 2022Quote: ... 140 mM NaCl, 1mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.1% Na-Deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 200 µL Roche cOmplete protease inhibitors). 0.5 mm diameter glass beads were added to 1 mm below the meniscus ...
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bioRxiv - Neuroscience 2022Quote: Cells were homogenized in 1 % NP40 lysis buffer (20 mM HEPES pH7.5, 150 mM NaCl, 1 % NP-40, 10 % Glycerol and Roche protease inhibitor mix) on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were collected and lysed in lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche Diagnostics, Mannheim, Germany]) at 4°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... the full-length Treacle was amplified by PCR from cDNA with primer set #1 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained XhoI sites ...
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bioRxiv - Biochemistry 2024Quote: ... tubes and homogenized for 1.5 minutes in 50μL of TBS containing 1% Triton X-100 (Table 1) and 1X protease and 1X phosphatase inhibitor cocktails (Roche, 04693124001 and 04906837001). The homogenates were then probe-sonicated with a Branson Sonifier® SFX150 (Emerson ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 1x cOmplete protease inhibitor cocktail [Roche-11697498001], 1 mM PMSF), incubated on ice for 10 minutes and centrifuged for 10 minutes at 21100 x g at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei pellets were resuspended in 100 µl of nuclei lysis buffer (20 mM HEPES pH 7.9, 0.1 M NaCl, 1mM EDTA, 1 mM EGTA, 1mM DTT, 25 % glycerol, 0.5 mM AEBSF, 1×Roche complete protease inhibitor cocktail) for 20 min at 4 °C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 0.1 mM EGTA, 100 mM NaCl, 1% Nonidet P-40, 10 mM Imidazole, supplemented with Roche protease inhibitor cocktail). Cell lysates were clarified by centrifugation at 10,000× g for 5 min at 4 °C and then incubated at 4 °C under constant rotation with His-ΔFIP1c-RCP559-649 fusion protein ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was extracted using NETN buffer (20 mM Tris-HCl pH 8, 420 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1 mM DTT, and Roche Protease Inhibitor Cocktail) and were resolved in 8% Bis-Tris SDS-PAGE gels ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8 and 10 mM NaF completed with 1 × protease inhibitor cocktail (Pierce, #A32955) and 1 × phosphatase inhibitor cocktail (Roche PhosSTOP, #490684001). The total protein concentration was determined using Bradford assay (Protein Assay Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.5% NP-40 [Thermo Scientific, Waltham, MA, USA], EDTA-free protease inhibitor [Roche, Basel, Switzerland]). Lysed astrocytes were collected into 1.5 mL microcentrifuge tube ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... to prepare for an hour long blocking in 10% lamb serum/TBST and an overnight incubation at 4°C in a 1% lamb serum containing Anti-Dig-AP antibody (Roche, 1:5000). The next morning ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in 400 μl of nuclear lysis buffer (50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 1% SDS, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet), and diluted by adding 800 μl of dilution buffer (16.7 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (Cytiva, clone BU-1) and rat anti-HA (Roche, clone 3F10) antibodies diluted 1:500 in block ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mM 1,4-Dithiothréitol (DTT) (Roche, Basel, Switzerland), 10 µM ROCK inhibitor Y27632 (ATCC® ACS-3030™ ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 protease inhibitor tablets (Roche, cat. No. 11836153001) and 1 ml BioLock (IBA ...
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bioRxiv - Biochemistry 2020Quote: ... 3 tablets of EDTA-free cOmplete inhibitor (Roche) were added to the media which was then centrifuged at 14,000 × g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 3 mg/ml Dispase II (Roche, Indianapolis, IN), and 1 mg/ml trypsin inhibitor (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 3 tablets complete protease inhibitor EDTA free (Roche) and 1 tablet PhosSTOP (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 U/ml dispase II (Roche Diagnostics, 4942078001), and 10 mM CaCl2 for 30 min at 37 °C with occasional shaking ...
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bioRxiv - Microbiology 2024Quote: ... with buffer 3 or the Taq polymerase (Roche). The initial denaturation for Taq polymerase at 95 °C for 5 min was followed by 30 amplification cycles of 1 min at 95 °C ...
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bioRxiv - Genetics 2023Quote: ... and dithiothreitol (Roche; cat. no. 3483-12-3). Lysates were rocked at 4° C for 20 min and centrifuged for 10 min at 15,000 × g ...
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bioRxiv - Molecular Biology 2023Quote: ... 3) Complete protease inhibitor cocktail (Roche, cat #11836153001) was included for final 1X concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... Complete EDTA-free protease inhibitor cocktail 3 (Roche), and PhosSTOP phosphatase inhibitor Cocktail (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Complete EDTA-free protease inhibitor cocktail 3 (Roche), and PhosSTOP (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... Primary antibodies were: rat-anti-HA (1:5,000; Roche Applied Science catalog ...
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bioRxiv - Cancer Biology 2021Quote: ... 5mM EDTA) with 1× cOmplete protease inhibitors (Roche 11697498001), incubated on ice for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche), after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman) ...
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bioRxiv - Cancer Biology 2021Quote: ... and collagenase D at 1 mg/mL (Roche, Switzerland) for 30 min at 37°C to obtain a single cell suspension.
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bioRxiv - Cell Biology 2020Quote: ... 1 mM NaVO3 and a protease inhibitor tablet (Roche)) ...
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bioRxiv - Genomics 2021Quote: ... 1% TX-100 and 1x complete protease inhibitor (Roche), digested first with DpnII (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% Triton X-100) containing protease inhibitor cocktail (Roche). Protein lysates were incubated and shaken on ice for 15 min and centrifuged at 12,000 × g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 tablet miniComplete EDTA free protease inhibitor (Roche) per 10 mL ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by biotinylated donkey-anti-sheep (1/500, Roche). ABC kit from Vector Laboratory was used followed by fluorescent tyramide staining (Alexa fluor 555 or 488 conjugated tyramide from Molecular Probes) ...
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bioRxiv - Developmental Biology 2020Quote: ... A 1/2500 dilution of anti-DIG (Roche, 1109327490) in 1x MABT + 20% heat inactivated sheep serum was then added and slides were placed in a humified chamber and incubated overnight at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 1% sodium deoxycholate) containing 1x protease inhibitor cocktail (Roche). Next ...