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Citations for Roche :
2451 - 2500 of 2544 citations for Mouse anti Mayaro Virus E2 M991 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Riboprobes were hybridized for 16 hr at 65°C and embryos were incubated with anti-DIG-AP (Roche, dilution 1/4000) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at room temperature and incubated with an appropriate antibody overnight at 4°C: 1:3000 anti-DIG-AP (Roche 11093274910), 1:1000 anti-DIG-POD (Roche 11207733910 ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used for the preparation of stained gels for expansion microscopy: Rat anti-HA (3F10, 1:25, Roche 12158167001), Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Molecular Biology 2024Quote: The following antibodies and dilutions were used for western blotting or immunofluorescence: horseradish peroxidase-linked anti-HA antibody (Roche, 1:5,000), rabbit anti-INTS4 (Bethyl Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were gradually washed into SSC buffer and then into PBT before overnight incubation with an anti-DIG primary antibody at 1:5000 (Roche #11093274910). Embryos were washed in PBT and then staining buffer before developing in BM Purple staining solution (Roche #11442074001) ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Neuroscience 2023Quote: ... we incubated slices with the secondary antibody (Alexa 488 donkey-anti-chicken, Immuno Jackson, 703-545-155) and DAPI (Roche, 10236276001) for 2h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Then embryos were incubated overnight at RT in AP-conjugated anti-DIG antibody (Roche, 1:1000 diluted in the blocking buffer). On the third day ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C in 1% goat serum in MABT with 1∶5000 alkaline phosphatase-coupled anti-Digoxigenin antibody (Roche Diagnostics). On the third day ...
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bioRxiv - Physiology 2024Quote: ... the samples were blocked with 5% skim milk in PBS-DEPC for 1 hour at 4°C and incubated with anti-DIG antibody (Roche Diagnostics) diluted 1:10,000 in the blocking buffer for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... unconcentrated cell culture supernatants were incubated with a rabbit monoclonal anti-glycodelin antibody (clone 12C9, kindly provided by ROCHE, Penzberg, Germany) for 1 h at room temperature prior to incubation with immune cells ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extract preparation and immunoblotting of Chlamydomonas cultures were performed as described previously (46, 48) with the following modifications: Antibody dilutions were: rat-anti-HA (1:5000) (3F10, Roche, Switzerland), Anti FLAG rabbit polyclonal (Rockland 600-401-383 ...
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bioRxiv - Genetics 2024Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (1:5000; anti-DIG-AP, Fab Fragments, Roche, 11093274910). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 1.5h in PBSTw-1%BSA before being incubated with anti-DIG-POD antibody (Roche, 1:2000) and other primary antibodies o/n at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein was equally loaded on SDS-PAGE for immunoblotting and the HA tag was detected using an anti-HA antibody (Roche, #11867423001 www.sigmaaldrich.com) and Femto chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ChIP followed by qPCR or Illumina sequencing was performed as described58 using the 3F10 rat anti-HA antibody (1:500; Roche no. 11867423001) to immunoprecipitate HA tagged AP2-HS ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then incubated for 1 h at 4° C in PBS + 1% BSA with a 1:1000 dilution of rat anti-HA monoclonal antibody (Roche; Basel, Switzerland) and washed 5×10 min on ice with PBS + 1% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then stained overnight at 4°C with pre-absorbed AP-conjugated sheep anti-DIG antibody (1:1000, Roche, cat. 11093274910) in PBS containing 0.1% Triton X-100 and 20% horse serum ...
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bioRxiv - Biochemistry 2021Quote: ... so that both DNA ends are blocked with 200 nM anti-digoxigenin Fab fragments (Roche Diagnostics, F. Hoffmann-La Roche Ltd, Switzerland) MutS heterodimers (50 nM monomer ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded human intestinal tissues was carried out on a Discovery Ultra automated slide stainer using a rabbit anti-human monoclonal antibody for CEA (Clone T84.66, Roche Glycart AG, Switzerland) at 2.23 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Genomics 2020Quote: ... Chemiluminescent detection of anti-DIG was performed using CDP-Star reagents from the DIG Northern Starter Kit (Roche # 12 039 672 910). Densitometry was performed in ImageJ.
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bioRxiv - Immunology 2021Quote: The measurement of anti SARS-CoV-2 neutralizing Abs was performed by electrochemiluminescence sandwich immunoassay (ECLIA) through Roche Elecsys Anti-SARS-CoV-2 S (Roche diagnostics, Switzerland). The neutralizing Ab were measured on a Cobas 601 modular analyzer (Roche diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Biochemistry 2020Quote: ... and the presence of enzymes were confirmed by immunoblot using the anti-His6 antibody conjugated to peroxidase (BMG–His-1 monoclonal antibody) (Roche, Mannheim, Germany).
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bioRxiv - Microbiology 2021Quote: ... 500 mM NaCl and 4 mM β-mercaptoethanol (β-ME)) in the presence of an anti-protease cocktail (Complete EDTA free, Roche) and 1 ul benzonase (MERK Millipore 70746) ...
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bioRxiv - Developmental Biology 2021Quote: ... Revelation of the dig-labeled ISH probe was then performed using an anti-Digoxigenin antibody coupled to POD (11207733910; Roche, 1/400) and revealed using a FITC-tyramide (1/400) ...
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bioRxiv - Developmental Biology 2022Quote: Single color staining of embryos hybridized with DIG-labelled riboprobes was performed using anti-DIG antibody conjugated to alkaline phosphatase (Roche, 1:6000) and BM purple (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight with 1:1,000 diluted alkaline phosphatase-conjugated anti-DIG sheep antibody (11-093-274-910; Roche Diagnostics, Basel, Switzerland) and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP ...
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bioRxiv - Genetics 2021Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Developmental Biology 2019Quote: ... These steps were followed by an incubation of the embryos in anti-digoxigenin-POD (peroxidase) Fab fragments (Roche, 1:50 in TNB). The riboprobe signals were finally detected using fluorescein-tyramide (FITC ...
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bioRxiv - Developmental Biology 2019Quote: ... The extracted PCR product was then used as a template for digoxigenin labeled cRNA probe transcription with T3 (anti-sense) and T7 (sense) RNA polymerases (Roche, Mannheim, Germany). The cRNA probes were purified using LiCI/ethanol precipitation and re-suspended in nuclease-free water before storage at −80 °C.
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...
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bioRxiv - Physiology 2021Quote: ... 1:500 goat anti-rabbit (AlexaFlour 488; A-11008) and 1:1000 4′,6-diamidino-2-phenylindole (DAPI; Roche 10236276001; Basel, Switzerland). After secondary incubations ...
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bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...