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Citations for Roche :
2451 - 2500 of 2944 citations for Archaemetzincin 2 AMZ2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
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bioRxiv - Microbiology 2020Quote: We obtained OP and NP samples from one hospitalised COVID-19 patient who had tested virus-positive three weeks earlier (cobas® SARS-CoV-2 test, Roche diagnostics), from three individuals who had previously tested SARS-CoV-2 positive ...
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bioRxiv - Cancer Biology 2021Quote: ... control and Capecitabine treated tumors were digested for 2 h at 37 °C with a cocktail of Collagenase I (Roche, Ref: 11088793001) and Hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor tissues up to 1 g were mechanically minced and digested in 2 ml RPMI1640 with 200 µg/ml Liberase TL (Roche by MilliporeSigma), 200 µg/ml Liberase DL (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... 0.2 μL of each primer (100mM stock) (Table SI-4) and 5 μL of 2× KAPA SYBR Fast Universal qPCR kit (KAPA Biosystems, USA). Samples were cycled (40 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellets were lysed in 200μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 5 mM MgAc, 2% digitonin, and 1X Roche protease inhibitor cocktail) by incubating on ice for 30 min and then diluted to 1ml with the lysis buffer containing 0.1% digitonin ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in French Press buffer (40 mM HEPES, 100 mM KCl, 20 mM imidazole, 2 mM PMSF, EDTA-free cOmplete protease inhibitor [Roche], pH 7.5) for 1 hour followed by homogenization using tight douncer ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared by scraping cells using 1X lysis buffer (10X recipe-50 mM Tris pH 7.5, Triton 20%, NP40 10%, 2 M NaCl, mixed with cOmplete protease inhibitor tablet - Roche, Product number 11873580001). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged for 10 min at 13,000 rpm 4°C to pellet the cell debris ...
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bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Cell Biology 2022Quote: ... and then homogenized on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol with MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 3 mM MgAc ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in lysis buffer (25 mM HEPES pH 7.5, 400 mM NaCl, 10 mM 2-mercaptoethanol, 0.1% Triton X-100 supplemented with lysozyme and Roche cOmplete protease inhibitor tablet), lysed by sonication (50% duty cycle ...
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Pathology 2021Quote: CCDs were dissected under stereomicroscopic observation either from fresh kidney slices (microperfusion) or after liberase treatment (Blendzyme 2, Roche Diagnostics, Meylan, France) (immunoblotting ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended in Buffer 2 (10 mM Tris-HCl pH=7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 10 min followed by a centrifugation step ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 2 mM MgCl2, 0.3 % Triton X-100, 0.25 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by sonication using Diagenode Bioruptor for 10 min (30 sec ON ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and transferred in an enzyme solution (2 mg/mL Collagenase/Dispase (Roche, Bale, Switzerland), 0,147 µg/mL TLCK (Lonza ...
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bioRxiv - Genetics 2021Quote: ... and cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C followed by a centrifugation step ...
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bioRxiv - Genetics 2021Quote: ... 30 million equivalent cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C and collected cells by a centrifugation step ...
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bioRxiv - Physiology 2021Quote: ... 1:500 goat anti-rabbit (AlexaFlour 488; A-11008) and 1:1000 4′,6-diamidino-2-phenylindole (DAPI; Roche 10236276001; Basel, Switzerland). After secondary incubations ...
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bioRxiv - Cell Biology 2021Quote: A reference sample was generated by lysing TK6 cells in DPBS with 2% SDS and cOMPLETE protease inhibitors without EDTA (Roche, 1x concentration) at 70 °C ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were resuspended in Lysis Buffer (25 mM HEPES pH 8.0, 250 mM NaCl, 10 mM imidazole pH 8.0, 5 mM 2-mercaptoethanol, 10% glycerol and supplemented with Roche cOmplete protease inhibitor) and sonicated ...
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bioRxiv - Molecular Biology 2021Quote: ... the probe for RNA FISH was prepared with 2 µg of BAC RP183A17 using Nick translation kit (10976776001) purchased from Roche (Basel, Switzerland). Salmon sperm DNA and cot1 DNA were added to the probe to mask the repetitive sequence ...
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bioRxiv - Microbiology 2020Quote: ... was cut into small pieces of approximately 2 mm3 in a petri dish using a sterile scalpel and transferred into a 2 ml MagNA Lyser bead tube (Roche, United Kingdom). Following this ...
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bioRxiv - Genomics 2020Quote: ... The fixed tissue was washed twice for 2 minutes with icecold PBS plus 1x cOmplete™ mini Proteinase Inhibitor (PI) Cocktail (Roche, 11846145001). The tissue was minced by the Dounce Tissue Grinder (Sigma ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The 1st PCR was performed in a 12-μL total volume of reaction mixture containing 6.0 μL of 2 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, MA, USA), 0.72 μL of forward and reverse primer (10 μM) ...
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bioRxiv - Immunology 2022Quote: ... colon tissue was homogenised in 350 µl of lysis buffer (40 ml of PBS, 10%FCS, 2 Complete Protease Cocktail Inhibitor Tablets (Roche, Mannheim, Germany). Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche ...
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bioRxiv - Microbiology 2022Quote: ... was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Neuroscience 2022Quote: ... were homogenized in 2 mL Precellys tube (Bertin Instruments) containing ceramic beads and 600 μL MagNaPure LC RNA Isolation Tissue buffer (Roche Life Science). The homogenate (400 μL ...
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Gut microbial disruption in critically ill patients with COVID-19 associated pulmonary aspergillosisbioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 infection was confirmed by quantitative reverse transcription PCR (TaqMan™-PCR performed on Roche cobas® 6800, Basel, Switzerland), performed on nasopharyngeal swabs or tracheal fluid ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The left lobe minced into small (~1-2 mm) sections and digested using 0.5 mg/mL collagenase D (Roche Diagnostics, Mannheim, Germany) and 50 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Each reaction was then added to Strep-Tactin®XT 4Flow® high capacity resin (IBA # 2-5030-010) pre-equilibrated with XB buffer supplemented with cOmplete™ EDTA-free Protease Inhibitor tablets (Roche #11873580001) and/or PhosSTOP™ Phosphatase Inhibitor Tablets (Roche # 04906845001) ...
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bioRxiv - Molecular Biology 2024Quote: ... while remaining cells were harvested in 500 µL lysis buffer (50 mM Tris pH6.8, 2% SDS, 1x protease inhibitor (Roche Complete EDTA-free)) snap-frozen and stored at -70 °C until further processing.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed by addition of 250 μl lysis buffer (50 mM Tris/HCl, 500 mM NaCl, 2% w/v SDS, add freshly 1 mM DTT, 1x Roche inhibitor cocktail) followed by incubation on ice for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were prepared by adding 1 volume of SDS lysis buffer (60 mM Tris-HCl pH 7.5, 2% SDS, 10% glycerol) supplemented with 1x protease inhibitor cocktail (Roche Diagnostics, Cat # 11836153001) and 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Neuroscience 2023Quote: Whole brains or dentate gyrus regions (isolated from the brain slices) were lysed in 2% sodium dodecyl sulfonate (SDS) with protease and phosphatase inhibitors (Roche Applied Sciences) and manually homogenized ...
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bioRxiv - Bioengineering 2022Quote: ... coli CFPS of GRFT was conducted at a 25 μL scale in 2 ml Eppendorf tubes using the PANOx-SP system utilizing a phosphoenolpyruvate (Roche, Indianapolis, IN), amino acids (Sigma Aldrich ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... minced finely and digested in pre-warmed PBS (with calcium and magnesium) with 2 mg/ml of collagenase A (Roche, Basel, Switzerland) for 1h on a rotating wheel at 37°C ...
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bioRxiv - Physiology 2023Quote: ... autofluorescence was quenched by treating paraffin-embedded sections with PBS/BSA (5%) for 2 h before performing TUNEL staining (Roche, Basel, Switzerland) according to the manufacturer’s protocol and using Proteinase K treatment ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... 1% 2-mercaptoethanol and 0.02% bromophenol blue) containing protease inhibitors (0469315900, cOmplete® Mini EDTA-free Protease Inhibitor Cocktail Tablets, Roche, Mannheim, Germany). The samples were separated by SDS-PAGE using 7.5% or 10% acrylamide gels at 100 V for about 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µl of the samples’ cDNA was mixed with 5 µl of FastStart SYBR Green Master (ROX; Hoffmann-La Roche, Basel, Switzerland), 0.25 µl of each primer (final concentration 500 nM) ...
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bioRxiv - Physiology 2024Quote: ... fixed with 4% PFA for 15 min and stained with 4′,6-diamidino-2-phenylindole (DAPI) (0.1 µg/mL; Roche, cat no. 10236276001) for 10 min at RT ...