Labshake search
Citations for Roche :
2451 - 2500 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... BHK cells were transfected with 3 μg of pBAC-SARS-COV-2 construct and1 μg of SARS-CoV-2 N (Delta) plasmid with the X-treme Gene 9 (X9) DNA transfection reagent (Roche: 6365809001) was added 1:3 as DNA:X9 ...
-
bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
-
bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
-
bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
-
bioRxiv - Systems Biology 2023Quote: ... HepG2s were washed once with 1X PBS then lysed in SDS lysis buffer (50 mM Tris HCl/2% SDS/5% glycerol/5 mM EDTA/1mM NaF/dH2O) supplemented with cOmplete Protease Inhibitor Cocktail Tablets (Roche #11836170001), Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich® #P5726) ...
-
bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
-
bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche, 04379012001), and a 370-bp fragment (nucleotides 1304- 1673 of NM_010610.3 ...
-
bioRxiv - Microbiology 2023Quote: ... The elution fractions were collected in tubes that were previously supplemented with 2 µl Protector RNase inhibitor (Roche Diagnostics, Karlsruhe, Germany). The elution fractions were split ...
-
bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
-
bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
-
bioRxiv - Immunology 2024Quote: ... were resuspended in mHBSS at a density of 107 cells/ml and pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM in a BSA-coated centrifugation tube ...
-
bioRxiv - Microbiology 2024Quote: ... BmE cells were transfected with 2 μg of this recombinant plasmid and A3 helper plasmid using X-tremeGENE HP DNA Transfection Reagent (Roche, Switzerland), and the culture medium was changed after 6 h ...
-
bioRxiv - Molecular Biology 2024Quote: Nuclei protein from MOLM-13-Cas9 cells transduced with either sgROSA or sgTOPORS-1 treated with 2 doses of DAC or vehicle over 24hrs was extracted in RIPA buffer supplemented with protease inhibitor (Roche, #04693159001). 10ug of protein was boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were washed three times with PBS and blocked with 2% Bovine Serum Albumin (BSA) Fraction V (Roche, Lot. # 69453726) in PBS and incubated at room temperature for 60 minutes ...
-
bioRxiv - Immunology 2024Quote: ... PBMC and BAL samples were thawed and rested for 2 hrs in PBS with 0.2 mg/mL DNase I (Roche Diagnostics GmbH) at 37°C with 5% CO2 to adhere monocytic cells ...
-
bioRxiv - Molecular Biology 2024Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (used only for CDKN1A ...
-
bioRxiv - Cell Biology 2024Quote: Unsynchronized population of worms or L4 larvae after aHS+3h were harvested in M9 from OP50 plates and the pelleted worms were lysed in equal volume of PBS Triton 2% + 10% PIC (Protease inhibitor cocktail, Roche 1183617), and glass beads (425-600µm ...
-
bioRxiv - Cell Biology 2024Quote: ... Eluates were transferred to fresh tubes and incubated for 2 h at 55°C with 0.3 µg/µl proteinase K (Roche, cat# 3115852001). For the chromatin input sample ...
-
bioRxiv - Genomics 2024Quote: ... with 2% BSA and resuspended in NEB+ (NEB with 2% BSA, 1U/ml proteinase inhibitor Roche #3335402001, and 1U/ml Protector RNAse inhibitor Roche #3335402001). Nuclei were extracted by douncing ∼800,000 to 1 million digested embryos in a steel tissue grinder (Wheaton Dounce DURA-GRIND 357572) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
-
bioRxiv - Microbiology 2024Quote: ... 1 mM EDTA, 1 mM DTT, 1 mM MgCl2, 1% digitonin, 6 μg/mL of DNAse/RNAse, 1x protein inhibitor Roche, 8 U mutanolysin, 8 mg/mL lysozyme) and incubated at 37°C for 30 min ...
-
Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 mm EDTA in ddH2O) supplemented with 1:25 cOmplete Protease Inhibitor Cocktail (Roche Diagnostic, catalog #11697498001) and 1:200 PMSF ...
-
bioRxiv - Cell Biology 2022Quote: ... 1% NP-40 for 10 min in the presence of complete protease inhibitor cocktail (Roche, 1:1000) on ice ...
-
bioRxiv - Neuroscience 2020Quote: ... 1% sodium deoxycholate and 1% Triton X-100) supplemented with 1xEDTA-free complete protease inhibitor mixture (Roche) and phosphatase inhibitor cocktails-I ...
-
bioRxiv - Developmental Biology 2020Quote: ... 1.5 mM MgCl2) with 1 mM PMSF and 1× EDTA-free protease inhibitor cocktail (PIC, Roche, 11873580001) for 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... in blocking buffer for 1 hour at room temperature followed by 1 hour of DAPI (Roche, # 33495822) staining ...
-
bioRxiv - Cancer Biology 2022Quote: ... The pellet was then resuspended in HEGX Buffer (20 mM HEPES-KOH, pH 7.2, 0.8M NaCl, 1 mM EDTA, 10% glycerol, 0.2% Triton X-100 and 1 Roche Complete Protease Inhibitor Tablet ...
-
bioRxiv - Genomics 2022Quote: ... 1 μL of 1:10 diluted cDNA was used in a 10 μL SYBR Green (Roche, 04887352001) qPCR reaction on a LightCycler 480 instrument (Roche) ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 % Nonidet-P 40) supplied with 1 tablet/50 mL c0mplete EDTA-free Protease Inhibitor Cocktail (Roche), per mg cell pellet and rotating on a wheel at 4°C for 15 minutes ...
-
bioRxiv - Developmental Biology 2021Quote: ... 0.1% NaDOC, 1% Triton X-100, 0.1% SDS, 0.25% sarkosyl, 1 mM DTT, protease inhibitors from Roche). The cells were sonicated on ice to shear DNA to 300-500 bp ...
-
bioRxiv - Immunology 2020Quote: Tissue was harvested and incubated in a digestion cocktail containing 1 mg ml−1 collagenase A (Roche) and 30 μg ml−1 DNase I (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 mM EGTA and 1% Triton X-100 supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at RT with the secondary antibodies together with DAPI (1:5,000; Roche), all diluted in Dako Antibody Diluent ...
-
bioRxiv - Microbiology 2022Quote: ... at a 1:1 ratio in a MagNA Pure 96-well deep-well extraction plate (06241603001, Roche), covered with a MagNA Pure Sealing Foil (06241638001 ...
-
bioRxiv - Cancer Biology 2023Quote: ... fixed sections were rehydrated and incubated with primary antibodies against ER (SP1; 1:1; Roche, Basel, Swiss), GATA3 (L50-823 ...
-
bioRxiv - Physiology 2023Quote: ... pregnant mice were anesthetized (1:1:0.16 Fentannyl [Fentadon, Dechra Vetinary Products UK], Midazolam [Hypnovel®, Roche, UK] and Ketamine [Ketavet ...
-
bioRxiv - Microbiology 2024Quote: ... Lungs were homogenized with 1 mm silica beads in 1 mL PBS using a MagNA Lyser (Roche). Homogenization was carried out at 6,000 rpm for 60 s and chilled on ice for 5 min in-between runs ...
-
bioRxiv - Genetics 2024Quote: ... 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate, 0.15 M NaCl, and 1×protease inhibitors, Roche) with slow rotation at 4 °C for 10 min ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.5 % NP-40 supplemented with 1 mM PMSF and 1 Complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland). The beads were washed three times with lysis buffer ...
-
bioRxiv - Microbiology 2024Quote: ... 0.1 uL-1 of 1:20 diluted DNA was mixed with 1X Kapa HIFI Hotstart Readymix (Roche) and 200 nM primers ...
-
bioRxiv - Plant Biology 2024Quote: ... followed by 1 hour antibody incubation (α-GFP, monoclonal mouse antibody, Roche, catalog no. 11814460001, 1:1000). After three washes with TBST for 10 min each ...
-
bioRxiv - Developmental Biology 2024Quote: ... Rabbit anti-ALFA (1:1000, NanoTag Biotechnologies, RRID:AB_3075998) and Mouse anti-HA (1:1000, Roche, RRID: AB_514505) primary antibodies ...
-
bioRxiv - Neuroscience 2024Quote: ... The agarose resin was washed once with high salt buffer (50 mM Tris HCl pH 7.4, 1M NaCl, 1 mM EDTA, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail and RNase Inhibitor ...
-
bioRxiv - Neuroscience 2024Quote: ... The resulting nuclei were resuspended in Wash Buffer (1 mL 1M HEPES pH 7.5, 1.5 mL 5M NaCl, 12.5 µL 2M spermidine, 1 Roche c0mplete Protease Inhibitor EDTA-free tablet with ddH2O to 50 mL ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 1 hour at room temperature and then probed with the HA-tag (Roche: 3F10; 1:4000) in PBS + 0.2% Tween-20 + 5% normal donkey serum overnight at 4°C ...
-
bioRxiv - Genetics 2024Quote: ... 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 1x Roche Complete Protease inhibitors ...