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Citations for Roche :
201 - 250 of 560 citations for Recombinant Human Membrane Metallo Endopeptidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Immunology 2019Quote: Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Microbiology 2019Quote: ... Membranes were probed with mouse anti-GFP (Roche, cat. no. 11814460001) (1:1,000 ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then incubated with HRP-conjugated anti-HA (Roche 3F10), or anti-actin (mAbcam 8224 ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche) (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA was blotted onto a positively charged nylon membrane (Roche) overnight using 10x SSC as a transfer medium ...
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bioRxiv - Neuroscience 2019Quote: ... the membrane was incubated with Anti-DIG-AP Fab fragments (Roche) for 25-30 minutes and washed three times with 1X washing buffer ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was washed with 1X wash buffer (Roche Applied Science) for 2 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was transferred to a positively charged nylon membranes (Roche, Switzerland) using established protocols ...
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bioRxiv - Microbiology 2020Quote: ... One gel was blotted to a PVDF (Polyvinylidene fluoride) membrane (Roche) by semi-dry Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed twice with 1x Washing Buffer (Roche, 115857262001) for 15 minutes ...
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bioRxiv - Genomics 2020Quote: The membrane slices were incubated with 40μL Proteinase K (3115828001, Roche) in 160μL PK Buffer (100mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were blocked with 0.5% (v/v) blocking reagent (Roche) in PBS containing 0.05% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were incubated with DIG Easy Hyb Granules (#11796895001, Roche) for 1 hr in a hybridization oven at 50°C ...
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bioRxiv - Plant Biology 2022Quote: ... RNAs were transferred to positively charged nylon membranes (Roche; Basel, Switzerland) by capillarity in SSC buffer as previously described (Sambrook et al ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked with 1:10 Western Blocking Reagent (Roche) in TBST (20 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... DNA was transferred on to positively charged nylon membranes (Roche, Germany). Hybridizations were performed at 68°C with 0.02% SDS ...
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bioRxiv - Cell Biology 2020Quote: ... Individual membranes were visualized using the enhanced chemiluminescence system (Roche Diagnostics).
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bioRxiv - Biochemistry 2021Quote: ... the membranes were transferred into 1× blocking buffer (Roche, REF:11096176001) and incubated at room temperature for 3 h ...
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bioRxiv - Microbiology 2020Quote: ... RNAs were then transferred to a positively charged nylon membrane (Roche) using a semi-dry transfer cell (Trans-blot®SD Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The PVDF membranes were blocked in 5% Western Blocking Reagent (Roche) then probed with primary and secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was incubated with 20 ml of blocking solution (Roche) for 30 min and then incubated with 20 ml of antibody solution (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... samples were transferred to a positively charged nylon membrane (Roche Diagnostics) by using an alkaline transfer procedure ...
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bioRxiv - Microbiology 2022Quote: ... Blocked PVDF membranes were incubated with anti-HA (1:2000, Roche), anti-MYC (1:3000 ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were incubated in blocking buffer containing anti-GFP antibody (Roche; Cat ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with an anti-digoxigenin-AP antibody (Roche, 11093274910 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with 1% Western Blocking Reagent (WBR; Roche #11921681001) or 5% BSA in TBST buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... before transfer to polyvinylidene fluoride (PVDF) membranes (IPVH00010, Roche Diagnostics, China) using standard procedures ...
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bioRxiv - Microbiology 2024Quote: ... which were then blotted onto positively charged nylon membranes (Roche, Germany). The RNA bands were fixed to the filter by UV-crosslinking for 2 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... 25ml of pre-warmed to 37°C EGTA buffer followed by 25ml of pre-warmed to 37°C EBS buffer with 2.3U of Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) were cannulated into the vena cava ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Microbiology 2021Quote: ... A second DNase I treatment to remove all residual genomic DNA was performed using 30 U of recombinant DNase I (Roche) and 10 U of Protector RNase inhibitor (Roche ...
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
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bioRxiv - Neuroscience 2021Quote: ... MGEs pooled from a single litter were incubated with 200 µL papain solution supplemented with 10 U/mL recombinant DNase I (Roche) for 10 minutes at 37 °C on a rocking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... An equal volume of lysis buffer and 110 U/ml of DNase (DNase I recombinant, RNase-free solution, Cat no. 4716728001, Roche) was added to the ground cell lysate followed by incubation for 1 hour at 4°C ...
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bioRxiv - Genetics 2022Quote: ... MYC-tagged or FLAG-tagged recombinant proteins were captured using ~5μg antibody and Protein-Agarose beads (Roche Applied Science, 11243233001) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Positive controls were generated by incubating some sections from control 3 dpf animals with recombinant DNAse I (400 U/ml; Roche) for 20 minutes at room temperature before the incubation in the reaction mix ...
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bioRxiv - Cancer Biology 2021Quote: ... + 0,4 g/l of human albumin (Vialebex) with Liberase TL (Roche) 150 ug/ml and DNase 1 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Human Bax cDNA was cloned in a pIVEX 2.4 plasmid (Roche). The cloning was done so that the His6 tag was absent from the construction ...
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bioRxiv - Genomics 2019Quote: ... using SeqCAP EZ Human Exome Library v3.0 (Roche NimbleGen, Madison, WI) for exome sequence capture ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and human epidermal growth factor 2 (HER-2) (790–4493, Roche).
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using the SeqCap EZ Human Exome Library (Roche) and a NovaSeq 6000 sequencer (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: Human genomic DNA of high molecular weight (Roche Diagnostics, Basel, Switzerland) were processed in a single replicate per DNA extraction protocol (Table 1) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Cross-linked membranes were blocked in pre-warmed DIG EasyHyb (Roche # 11603558001) at 43°C for four hours rolling in hybridization tubes ...
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bioRxiv - Molecular Biology 2020Quote: PCR products were transferred to a positively charged nylon membrane (Roche, Switzerland) using capillary transfer under denaturing conditions ...