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Citations for Roche :
201 - 250 of 646 citations for Powassan Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were detected with Anti-c-myc-Peroxidase (Roche #11814150001) at dilution of 1:5,000 ...
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bioRxiv - Neuroscience 2020Quote: ... the proteins were transferred onto a PVDF membrane (Roche Diagnostics, Mannheim, Germany). Cofilin1 and phospho-Cofilin1 (Ser3 ...
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bioRxiv - Molecular Biology 2020Quote: ... HA-tagged proteins were detected using 1:1,000 anti-HA antibody (Roche) and 1:5,000 anti-rat antibody (Abcam) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were lysed and sonicated in RIPA buffer with protein inhibitors (Roche). Protein concentrations were estimated by BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently proteins were digested by addition of proteinase K (Roche #03115852001) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The relipidated proteins were treated with 1 mM AMPPNP (sodium salt, Roche) for overnight at 4 °C and desalted with a PD-10 column ...
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bioRxiv - Plant Biology 2022Quote: ... and proteins were eluted by using 0.25 mg/ml HA peptide (Roche). HA- and FLAG-tagged proteins were immunologically detected using HRP-conjugated anti-HA 3F10 (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Immune complexes were captured using 20 µl Protein A agarose beads (Roche, previously saturated with 1 mg/ml BSA and 1mg/ml yeast tRNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and total serum protein (TSP) were determined using kits supplied by Roche Diagnostics and the Roche/Hitachi Analyzer machine at Al-Aulaqi Specialized Medical Laboratory ...
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were detected using anti-myc antibody (mouse monoclonal; Roche) di-luted 1:5000 (v/v) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected using monoclonal anti‐GFP (mouse; 1:3000; Roche 11814460001). Secondary antibody was HRP‐linked anti‐mouse polyclonal (goat ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were extracted using EB2 supplemented with 1X PhosSTOP (Roche, Indianapolis, IN) and FLAG immunoprecipitation was performed using anti-FLAG M2 agarose (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were immunoprecipitated using a monoclonal anti-GFP antibody (Roche). Analysis of enrichment of target genes was performed by qPCR using the oligos listed in the Table S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were digested with 0.04 mg/mL proteinase K (Roche #03115852001). DNA was recovered using a DNA purification kit (Bioline #52060) ...
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bioRxiv - Neuroscience 2022Quote: ... protein G-Sepharose Fast Flow and immobilized streptavidin Mutein Matrix from Roche; protein molecular weight standards from Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were extracted using RIPA (Triton 1%) buffer supplemented with protease (Roche) and phosphatase inhibitors (Roche).
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bioRxiv - Plant Biology 2022Quote: ... After last wash proteins were cleaved by adding sequencing grade trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellets were suspended in in cOmplete Protease Inhibitor Cocktail (Roche) containing radio-immunoprecipitation assay (RIPA ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μl of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Fusion proteins were detected with α-Myc monoclonal antibodies (Roche, Stockholm, Sweden). The following constructs were used ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μl of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μL of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... and subsequently incubated with 20 μL of protein G-agarose beads (Roche) or protein A-agarose beads (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then degraded by addition of 50 µg Proteinase K (Roche) and incubation at 56°C for 90 minutes ...
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bioRxiv - Cell Biology 2023Quote: The TetR-eYFP tagged proteins were transfected using the XtremeGene-9 (Roche) transfection reagent according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: We purchased the following purified ECM proteins: fibronectin from Roche (Cat# 11051407001), laminin from Sigma‒Aldrich (Cat# L6274) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by affinity isolation with Protein A agarose beads (Roche, PROTAA-RO) overnight at 4°C on an end-over-end rotator ...
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bioRxiv - Genetics 2024Quote: Protein extracts were prepared in RIPA buffer containing protease (Complete Mini, Roche) and phosphatase (PhosSTOP ...
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bioRxiv - Developmental Biology 2024Quote: ... AFSC-EV protein was extracted via a phosphatase and protease inhibitor (Roche) containing tissue extraction buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cell lysates were incubated further with protein G sepharose beads (Roche Applied Bioscience) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Pull-down was performed with 30 µl protein-G-agarose bead slurry (Roche) for 1 hr at 4°C under gentle agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of protein lysate was incubated with 1μg anti HA-antibody (Roche) overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) was used in the immunostaining assay.
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bioRxiv - Plant Biology 2021Quote: Protein blots were probed either directly with a Streptavidin-alkaline phosphatase conjugate (Roche) or with antibodies raised against GFP (Sicgen ...
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bioRxiv - Plant Biology 2020Quote: ... The immunoprecipitated proteins were detected by Western-blotting using anti-HA antibody (Roche) and anti-Flag antibody (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... followed by a 2 h incubation with Protein A Agarose beads (Roche, 11719408001) at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and protease and phosphatase inhibitors to isolate insoluble proteins and S7 nuclease (Roche) to release DNA bound proteins ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected with primary antibodies mouse mAb α-GFP (Roche Diagnostics #11814460001), 1:1000 and rabbit α-PfHP1 12 ...
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bioRxiv - Biochemistry 2021Quote: Protein was collected from cells lysed in RIPA buffer with protease inhibitor (Roche). Protein extracts were passed through a 25g needle to break up DNA and subsequently quantified using BCA assay ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were electroblotted to PVDF membrane and probed with specific antibodies: αHA (Roche), αGFP (Milteny Biotech) ...
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bioRxiv - Cell Biology 2022Quote: ... Antigen-antibodies complexes were pulled down using Protein G-Agarose (Roche, Lot #70470320). First ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted in the extraction buffer containing protease inhibitor cocktail (Roche) as described above ...
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bioRxiv - Cell Biology 2019Quote: ... Soluble protein fractures were then mixed with 5x sample loading buffer (Roche, USA) and boiled for 5 min ...
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bioRxiv - Cell Biology 2019Quote: GFP-Pav and Tum proteins were incubated with mouse anti-GFP antibody (Roche) overnight at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... and protein was removed by digestion with 1.2 mg/mL proteinase K (Roche) in proteinase K buffer (50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: Protein was extracted by RIPA buffer supplemented with protease inhibitor cocktail tablets (Roche). The proteins were separated in 10% SDS-PAGE with Mini-PROTEAN Electrophoresis System (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... Proteins were then digested using 88 μg/mL of Proteinase K (03115828001, Roche) during 1 hour at 65°C ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... proteins were digested for 18 h at 37°C with chymotrypsin (Roche, Germany), followed by 3 h at 37°C using trypsin (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... 750 μgs of protein were incubated with 20 μL of FLAG-beads (Roche) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by digesting the RNA with 3 µg RNase A (Roche) and 30U RNase T1 (Roche ...