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Citations for Roche :
201 - 250 of 1062 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 5% (v/v) glycerol) per g cell wet weight containing cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland) and lysed via the Multi Cycle Cell Disruptor (Constant Systems ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% (v/v) Triton X-100) to leave only matrix before treatment with 10μg/ml DNAse 1 (Roche) to cleave phosphodiester linkages in the DNA backbone ...
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bioRxiv - Systems Biology 2020Quote: ... 0.5% bovine serum albumin (Fraction V, Roche) and 14.5 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... with 2% BSA fraction V (Roche-Diagnostics), 50 mmol/L 2-mercaptoethanol,10 mmol/L nicotinamide (Calbiochem) ...
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bioRxiv - Cell Biology 2021Quote: ... 2% bovine serum albumin (Fraction V, Roche) in PBS for 1 hour at RT ...
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Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... 1% [w/v] blocking reagent (Roche, Germany), and 20% [v/v] formamide ...
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bioRxiv - Bioengineering 2022Quote: ... bovine serum albumin fraction V (10735078001; Roche), β-mercaptoethanol (M3148 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v))% NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Microbiology 2020Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v) % NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... and homogenized in 100 μl ice-cold homogenization buffer (1% [v/v] SDS, 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 10 mM DTT and a Roche Complete protease inhibitor 1 tablet/10 ml) ...
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bioRxiv - Immunology 2022Quote: ... The cells were lysed in TBS/1% (v/v) IGEPAL-630 (sigma) containing 1X Complete protease cocktail inhibitors (Roche). The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were washed in cold PBS and resuspended in 0.5% v/v Triton X-100 in PBS supplemented with a cocktail of protease (Roche) and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Livers and liver tumours were excised and then minced with scissors before incubation in RPMI/10% (v/v) FBS/2mM EDTA/1.2mg/mL Collagenase D (Roche)/1mg/mL DNase I (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in PBS supplemented with 0.5% (v/v) Triton X-100 and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) (PI) ...
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bioRxiv - Neuroscience 2023Quote: ... skin tissues were homogenized at a 10% (w/v) concentration in a lysis buffer containing 2 mM CaCl2 and 0.25% (w/v) collagenase A (Roche) in Tris-Buffered Saline (TBS) ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% (v/v) NP-40 and 0.5 mM Tris carboxy ethyl phosphene (TCEP) buffer supplemented with protease inhibitor cocktail (Roche), flash-frozen in liquid nitrogen and lysed under cryogenic conditions using a Retsch MM400 (Retsch) ...
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bioRxiv - Biophysics 2022Quote: ... culture medium was collected and cells were gently washed twice with 5 ml cold PBS and lysed in 0.5 ml of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001], PhosSTOP [Roche, PHOSS-RO] ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.0, 1 mM EDTA, 10 mM DTT, 0.05% [v/v] Triton X-100 and 1x Protease Inhibitor Cocktail [Roche]). Crude leaf extracts were incubated for 2 h at 4 °C on a turning wheel and centrifuged at 16.900 g for 5 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: Bacterial loads were evaluated by mechanical disruption of organs in 0.05% v/v Tween 20 in PBS containing a proteinase inhibitor cocktail (Roche) prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibody solution was then replaced with DAPI solution (Roche, 10236276) in the dark at room temperature for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Embryos were rinsed and blocked in TNB solution (2% blocking solution (Roche) in TNT ...
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bioRxiv - Developmental Biology 2023Quote: ... in Wash+ solution (Wash solution supplemented with 0.5 mM Spermidine and Roche complete Protease Inhibitor Tablet 1 tablet per 50 mL) ...
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bioRxiv - Cell Biology 2021Quote: ... TSA blocking solution (Roche) was used at 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... SybrGreen master solution (Roche) was used and qPCR was run in StepOne Real time equipment (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... NBT/BCIP solution (Roche) was used as the chromogenic substrate.
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% blocking solution (Roche)) containing 10 nM AF568-conjugated C-rich telomeric PNA probe (TelC-AF568 ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Cell Biology 2022Quote: Cells were harvested in cold PBS and subsequently lysed in 1% (v/v) Triton X-100 in PBS supplemented with Protease Inhibitor Cocktail (Roche), N-ethylmaleimide and sodium orthovanadate ...
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bioRxiv - Genetics 2021Quote: Animals were resuspended in lysis buffer (50 mM Tris/HCl pH 7.4, 100 mM NaCl, 1% v/v SDS, complete protease inhibitor cocktail (Roche Diagnostics)) ...
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bioRxiv - Molecular Biology 2023Quote: Thawed and washed cell pellets were lysed in 1 mL sterile PBS with 10% (v/v) glycerol and 1x cOmplete mini protease inhibitor cocktail (Roche). Ten micrograms of protein were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 10 min on ice in 1% (v/v) Triton X-100 in TBS supplemented with protease inhibitor (cOmplete, Roche) and phosphatase inhibitor (PhosStop ...
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bioRxiv - Molecular Biology 2022Quote: ... re-suspended in buffer A (50 mM HEPES/NaOH pH8, 500 mM NaCl, 10% (v/v) glycerol) supplemented with protease inhibitors (Roche, complete mini ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP 10 % v/v glycerol) supplemented with 4x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and Supplementarylemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... cells were gently washed twice with 500 μl cold PBS and directly lysed in 50 μl of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... N’-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Genetics 2022Quote: ... COS-7 cells transiently expressing POPDC constructs with the appropriate epitope tag were lysed using a 1% (v/v) Triton X-100 based lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche). Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 mM imidazole and 10 % (v/v) glycerol] supplemented with 1 mM PMSF and complete EDTA-free protease inhibitor cocktail (Roche). After centrifugation at 45 000 x g supernatant was filtered (0.45 µm ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% (v/v) Tween-20) supplemented with 10 μg/mL DNase I and 1 cOmplete protease inhibitor cocktail tablet (Roche) per L buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody incubation was performed for 16 h at 4°C in AB buffer supplemented with 1% v/v Fetal Calf Serum and anti-DIG antibody coupled to alkaline phosphatase (1∶5000 dilution; Roche). Sections were then washed thoroughly in AB and equilibrated in alkaline phosphatase buffer (AP - 0.1 M Tris–HCl pH ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10% glycerol v/v) supplemented with 1 mM DTT and 1 x cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysates were then clarified by centrifugation at 14000g for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with 0.2% (w/v) collagenase II (Roche) in DMEM containing 20% FCS and antibiotic-antimycotic (100× ...