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Citations for Roche :
2351 - 2400 of 8118 citations for 7 R AMINO PHENYL ACETAMIDO 3 METHYL 3 CEPHEM 4 CARBOXYLIC ACID DIMETHYLFORMAMIDE 2 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... amplified using primers LN112 and LN113 (Table 2) and the PCR DIG Synthesis Kit (Roche, Basel, Switzerland) as a probe ...
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bioRxiv - Developmental Biology 2024Quote: Embryos were deyolked in cold-DPBS supplemented with cOmplete proteinase inhibitor cocktail (Roche, used at 2× concentration), extracted with RIPA buffer (50 mM Tris ...
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bioRxiv - Genomics 2024Quote: ... the embryos were transferred to pre-hybridization buffer (50% deionized formamide, 5x SSC pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Bioengineering 2024Quote: ... Pre-hybridization was carried out at 48°C for 2 hours in DIG Easy Hyb buffer (Roche). The trnI/A probe was denaturalized for 20 minutes at 68°C and used for hybridization overnight at 48°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM EDTA (pH 8)) supplemented with 2 mM Na3VO4 and complete protease inhibitor mixture (Roche). Lysates were first centrifuged at 4 °C for 15 min at 20.000 g and subsequently subjected to ultracentrifugation at 4° C for 30 min at 100.000 g ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were treated at 37 °C for 2 h with 500 µL of RNAse A (Roche) 2 mg/mL and after 30 min with 200 µL of pepsin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: The beads were supplemented with a PCR mix comprising 20 μl of 2× HotStart Readymix (Kapa Biosystems), 4 μl of 5′ end biotin-modified P7 primer (10 μM ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1, Roche) and protein G sepharose beads or ALFA selector ST beads (NanoTag Biotechnologies) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl of target RNA were incubated for 15 min with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 7.5) and placed in RBR buffer (20% Goat serum, 2% Roche Blocking Reagent in 1x MAB) on the orbital rocker for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... 80 mM KCl, 0.2 mM spermine, 5 mM 2-ME, 0.5 mM spermidine, 0.2% IGEPAL CA-630, Roche mini EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2023Quote: ... after cutting SVF into small pieces and incubation in a 2 mg/ml collagenase D solution (Roche) at 37°C in a water bath for 45 min ...
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bioRxiv - Immunology 2024Quote: ... plus 2% fetal calf serum (Dutscher)) supplemented with 50μg/mL DNAse and 10μg/mL collagenase D (Roche).
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bioRxiv - Biochemistry 2021Quote: ... 137.5 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM sodium fluoride, EDTA-free protease inhibitor cocktail [Roche]). 1 mM orthovanadate was added to the lysis buffer to prevent binding of substrates to the PTP1B trapping mutant ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in PBS-E LB (1x PBS, 1% TX100, 1 mM EDTA, 1 mM DTT, Protease Inhibitor cocktail (Roche) and Phosphatase Inhibitor solution (Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were lysed in 300 µL FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 mL ceramic beads using a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cell Biology 2021Quote: ... Heads were homogenised in 1 mL BRB-80 lysis buffer (80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT, cOmplete EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were lysed in 300 µL FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 mL ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2, 10% glycerol, 1% Tween-20, 20 mM imidazole, 1 mg/ml lysozyme, and 1x protease tablet [Roche]) for thirty minutes on ice with 5 min intervals of 10 s vortexing ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Biochemistry 2023Quote: ... α-Ape1 (1:5000) (Hamasaki et al., 2003), α-Prb1 (laboratory stock, 1:5000) and α-GFP (Roche, 11814460001, 1:1000), were used as primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM NaF, 1 mM EDTA, 1 mM EGTA, and 1% Triton X-100, supplemented with Complete EDTA-free protease inhibitor [Roche]). Cell samples were rotated for 10 min at 4 °C to achieve lysis ...
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bioRxiv - Developmental Biology 2023Quote: Spinal cords from wt and Sorcs2-/- mice were dissected and lysed on ice with TNE lysis buffer (Tris-base 10 mM, NaCl 150 mM, EDTA 1 mM, NP40 1%, cOmplete 1% (Roche), PhosSTOP 1% (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 1% (w/v) sodium deoxycholate and 1% (v/v) Triton X-100) with 1% (v/v) protease inhibitor cocktail (Roche). After homogenization ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton-x-100 containing 1 EDTA-free protease inhibitor tablet (Roche) and 1% phosphatase inhibitor cocktails 1 and 2 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 1 Complete Mini Protease Inhibitor Cocktail tablet (Roche) were added to 10 mL buffer ...
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bioRxiv - Microbiology 2020Quote: ... anti-3xHA (1:2000, 12CA5, Roche or 1:4000, C29F4, Cell Signaling) anti-PfALD (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... 1° antibodies used: α-GFP 1:1000 dilution (Mouse, Roche, Cat.No. 11814460001), α-Tubulin (TAT1 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 % (v/v) NP40 and 1 x cOmplete Protease inhibitor cocktail (Roche)) by incubating on ice for 30 min ...
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bioRxiv - Genomics 2021Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland) and eluted in Tris-Cl ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM EDTA and supplemented with 1 × complete protease inhibitor cocktail (Roche). DDM/CHS detergent (1%/0.1% ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA ...
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bioRxiv - Microbiology 2022Quote: ... 10U ml−1 DNase 1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... DNase 1 10U mL−1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... 10U mL−1 DNase 1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland). Genomic libraries were generated using Kapa Hyper library prep kit (Roche ...
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bioRxiv - Immunology 2023Quote: ... 1% Triton X-100) containing 1×EDTA-Free Protease inhibitor cocktail (Roche) and 1 mM PMSF (Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... 1 mM PMSF and 1×cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche) were added throughout the above procedures ...
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bioRxiv - Genetics 2023Quote: ... 1 mM EDTA and 1 x cOmpleteTM mini protease inhibitor cocktail (Roche). PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Bound proteins were eluted with a 30 minute incubation at 4°C with Elution Buffer (50 mM Tris pH 8.0, 40 mM reduced glutathione, and Roche Complete protease inhibitors). At the end of the incubation ...
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bioRxiv - Cell Biology 2019Quote: ... cells were harvested through centrifugation at 5000 rpm for 10 min at 4°C and afterwards washed with 2mL PBS supplemented with 1x protease inhibitors cocktail (Roche, Basel, Switzerland). Cell pellet was resuspended in lysis buffer (150 mM KCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 40 mM Tris-HCl pH 7.5, 20 mM MgCl2, and 4% Triton X-100 supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail), and 24 ml nuclease free water on ice for 20 min with frequent mixing ...