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Citations for Roche :
2351 - 2400 of 3510 citations for 4 Chloro 2 iodothieno 2 3 b pyridine 5 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... all membranes were blocked with 5% Western Blocking Reagent (Roche, Indianapolis, IN, USA) in TBST ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the following were mixed: 5 μL Kapa HiFi buffer 5X (Kapa Biosystems, USA), 0.75 μL dNTPs 10 μM ...
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bioRxiv - Immunology 2021Quote: ... and 5 mM Na3VO4) in the presence of complete Protease Inhibitor Cocktail (Roche). The cell lysates were incubated on ice for 30 min and were then centrifuged for 20 min at 15,000 rpm at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol and 10 mM imidazole supplemented with EDTA-free protease inhibitor (Roche). The cell suspension was lysed by ultrasonication and the lysate was cleared by centrifugation at 40,000 x g for 40 min ...
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bioRxiv - Immunology 2020Quote: ... Cytokines were added at the following concentrations: GM-CSF (5 ng/mL, Roche), TNF (10 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... 5) Incubated with 50μL of TUNEL reaction mixture for one hour (Roche protocol); 6 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5% glycerol) containing a protease inhibitor cocktail (Roche,11 697 498 001). The cell lysates were further sonicated on ice for 10 seconds ...
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bioRxiv - Microbiology 2022Quote: ... and blocked at room temperature with either 5% bovine serum albumin (BSA, Roche), 5% milk or Odyssey buffer (1:1 ...
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bioRxiv - Cell Biology 2022Quote: ... and 5% glycerol) containing 1× complete EDTA-free protease inhibitor cocktail (Roche 1187358001) and lysed in an Emulsiflex-C5 cell disruptor (Avestin) ...
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bioRxiv - Systems Biology 2024Quote: ... then 5 min at 62°C) with KAPA HiFi HotStart Ready Mix (Roche). Libraries were quantified by Bioanalyzer (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.6 with KOH) containing 5 mg ml−1 collagenase (type A, ROCHE). Defolliculated oocytes were injected with 50 ng mRNAs of mixed GlyT1 ...
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bioRxiv - Immunology 2023Quote: ... 5 mM EDTA] supplemented with 1x protease inhibitors (Mini Protease Inhibitor Tablets, Roche). Total protein concentration was determined by BSA Protein Assay Kit (Thermo Life) ...
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bioRxiv - Immunology 2024Quote: ... Cell nuclei were stained with 5 µg/ml of DAPI (10236276001, Roche Diagnostics).
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bioRxiv - Genetics 2023Quote: ... 5 mM Tris-HCl (pH 7.4) supplemented with Protease Inhibitor cocktail (Roche, #04693159001) and transferred to a 7 mL Dounce tissue grinder (KIMBLE KONTES ...
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bioRxiv - Developmental Biology 2023Quote: ... using a 5 μl reaction mixture of DNA SYBR Green I Master (Roche) according to the standard manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM beta-mercaptoethanol (BME)) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48.384g for 45 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 5% glycerol) supplemented with Complete Mini protease inhibitor mixture tablet (Roche Applied Science) and 10 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Genetics 2023Quote: ... Each reaction consisted of 5 μL LightCycler 480 SYBR Green Master mix (Roche), 0.5 μL each of the forward and reverse primers (100 μM ...
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bioRxiv - Biochemistry 2023Quote: ... 5% glycerol) containing a protease inhibitor cocktail tablet (Complete EDTA-free, Roche Diagnostics) and gently stirred for 30 min in the presence of 0.2 mg/mL lysozyme (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM beta-mercaptoethanol (BME)) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48,000g for 45 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and 5% (v/v) glycerol) supplemented with protease inhibitor cocktail tablets (cOmpleteTM, Roche). The suspensions were kept on ice ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared lysate was loaded onto a Ni2+- NTA column (5 ml, Roche) prewashed with lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... media was replicated by a 5% solution of WST-1 reagent (Roche, 11644807001) in media ...
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bioRxiv - Developmental Biology 2024Quote: ... uridine-5’-triphosphate (UTP) and T7 /SP6 RNA polymerase (Roche, Cat. 10881775001/10810274001).
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bioRxiv - Developmental Biology 2021Quote: ... They are incubated for at least 4 hours in blocking solution 1x WBR (Roche, 11921673001) and incubated overnight at 4°C with anti-Digoxigenin antibody coupled to alkaline phosphatase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: For sequencing all samples were treated with 4 mg/ml of proteinase K (Roche Diagnostics). RNA and small RNA were extracted and enriched in separate fraction from the supernatants using the mirVana kit according to manufacturer’s instructions to separate samples into two groups ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μL of the supernatant were incubated with 20 μg/mL proteinase K (PK; Roche) in a total volume of 22 μL RIPA buffer for 1 hr at 37°C to digest all proteins except for PK-resistant PrPSc ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed at 4°C in RIPA buffer supplemented with protease inhibitors (Roche, 4693159001). Protein concentrations were measured using a BCA Assay Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed 4 times in wash buffer containing 25% formamide (Roche, 11814320001), 2x SSC (Ambion ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were incubated overnight at 4°C with Anti-Digoxigenin-AP antibody (1:2000, Roche) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in horseradish peroxidase-coupled anti-DIG antiserum (11207733910, Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... and visualized after incubation with substrate solution containing NBT (4-nitro blue tetrazolium, Roche 11383213001) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were deparaffinized and rehydrated before digestion with proteinase K (4 μg/ml; Roche) for 15 min at 37℃ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were subsequently fixed with 4% paraformaldehyde (PFA) and labelled with TUNEL (Roche, cat# 11684795910). Cells were then immunostained (1:2000 ...
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bioRxiv - Biochemistry 2023Quote: ... diluted in 100 mM ammonium acetate (pH = 4) and digested with Glu-C protease (Roche) at 10:1 protein:enzyme for 1 h at room temperature prior to mass spectrometric analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were fixed in freshly made 4% PFA in PBS with PhosSTOP™(Roche, 4906845001) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µM MgSO4 and the protease inhibitor cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Cells were then sonicated and the mixture was clarified by centrifugation ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with anti-digoxigenin antibody (1:5000, Roche 11093274910). Following several washes to remove excess antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were subjected to end-on rotation at 4 °C with anti-GFP (Roche, 11814460001) or anti-FLAG (Merck ...
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bioRxiv - Biophysics 2024Quote: ... PCR reactions were performed as follows: 4 µL of KAPA HiFi HiFi HotStart ReadyMix (Roche) was assembled with 1.6 µL of 1:10 bacteria dilution ...
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bioRxiv - Cell Biology 2024Quote: ... All steps were performed at 4°C and in the presence of Protease inhibitors (Roche) and 1mM TSA in every buffer to prevent protease and HDAC activity ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T cells or 0.75 million primary neurons using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Rap1GAP- RlucII/rGFP-CAAX + Gαi2 and D2 or PDZ-RhoGEF-RlucII/rGFP-CAAX + Gα13 and TPαR) using X-tremeGENE 9 DNA transfection reagent (3:1 reagent/DNA ratio; Roche) diluted in OptiMEM (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected hippocampi were dissolved in lysis buffer (1M Tris-Cl, pH 7.5; 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001 ...
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bioRxiv - Biophysics 2020Quote: ... The 3′ end of the 1,882-nt DNA handle was labeled with dig-ddUTP using terminal transferase (Roche), and the 798-nt DNA handle of the transcript was functionalized with biotin on the 5′ end of the PCR primer ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were harvested and lysed in IP buffer (1 x PBS, 3 mM KCl, 2.5 mM MgCl2, 0,5 % Triton X-100 and protease inhibitors from Roche). 35 μl of this lysate was loaded onto an SDS-gel (lysate lanes) ...
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bioRxiv - Cell Biology 2022Quote: ... The sonicated sample was layered over 3 ml of S3 solution (0.88 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) in a new Falcon tube and centrifuged at 3000 × g for 10 min at 4°C ...