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Citations for Roche :
2301 - 2350 of 3373 citations for Mouse Anti Hepatitis C Virus E1 Antibody 1879 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (3F10, Merck Millipore/Roche), and anti-α-Tubulin (B-5-1-2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sheep anti-DIG (1:25; Roche) was used as primary antibody and Alexa Fluor conjugate (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-DIG-alkaline phosphatase (AP) (Roche) and anti-fluorescein-AP (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryonic tail biopsies were used for rapid genotyping using KAPA Mouse Genotyping Kits (Kapa Biosystems, KK7352). All mouse protocols and experiments were approved by the Rutgers Institutional Animal Care and Use Committee ...
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bioRxiv - Immunology 2021Quote: ... mouse tracheas were surgically removed and placed in 10 ml 0.15% pronase solution (Roche, Basel, CH) and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: The lipid analysis of mouse plasma samples was performed using the COBAS Integra 400 Plus (ROCHE) and the following kits ...
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bioRxiv - Pathology 2020Quote: ... Quantitation of urinary albumin and creatinine was carried out using mouse albumin-specific ELISA kits (Roche) and creatinine determination kits (Enzymatic Method ...
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bioRxiv - Pathology 2021Quote: DNA extraction and PCR were performed using Kapa mouse genotyping kit (Kapa Biosystems, Wilmington, MA, USA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: Mouse lungs were isolated and placed in RPMI1640 containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNase I (50 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... mouse thymuses were cut into small pieces and digested with 0.2 mg/mL DNase I (Roche) and 0.2 mg/mL collagenase P (Roche ...
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bioRxiv - Immunology 2023Quote: Mouse lungs were isolated and placed in RPMI1640 containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNase I (50 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... mouse ventricular cardiomyocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: Mouse ventricular myocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30mg of mouse TA muscle was added to MagNA Lyser Green Bead tubes (Roche 03358941001) with 600ul lysis buffer (PureLink RNA Mini Kit ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Cell Biology 2022Quote: ... The remaining tissue was digested on a shaker at 400 rpm for 40 min at 37°C in 100 μg/ml Liberase TM (Roche, 5401127001) and 100 μg/ml DNAse I (Roche ...
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bioRxiv - Genomics 2019Quote: ... and AdRb (5′ -TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16°C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65°C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: Total protein lysates from MDDC and cDC cultured for 1 h in the presence of media or individual or combined Poly I:C and 2′3′-di AM(PS) agonists were obtained using RIPA buffer containing 1% phosphatase and protease inhibitors (Roche Diagnostics). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001). Anti-FLAG M2 Affinity Gel (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... HindIII treatment was performed at 37°C for 1 h in 1x reaction buffer B with 10U of HindIII enzyme per reaction (HindIII, 10656321001, Roche, Switzerland). Mung bean nuclease treatment was performed at 30°C for 30 min in 1x mung bean nuclease reaction buffer with 1U per reaction (Mung Bean Nuclease ...
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bioRxiv - Plant Biology 2021Quote: ... The digested DNA was separated in a 1 % agarose gel at 50 Volts for 72 hours at 4°C and transferred to a nylon membrane (Roche®) overnight ...
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bioRxiv - Genomics 2020Quote: ... for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2, 70% formamide, 0.5% Roche 11096176001 blocking reagent). Hybridization was carried out for 2 hours at RT in the dark ...
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bioRxiv - Immunology 2022Quote: Spleens or lymph nodes were harvested and submitted to enzymatic digestion for 25 minutes at 37°C with Collagenase IV (Wortington biochemicals) and DNAse I (Roche Diagnostics). Organs were then mechanically digested and passed over 100µm cell strainers (Corning) ...
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bioRxiv - Cancer Biology 2022Quote: ... following a 30 min incubation at 4 °C in the presence of protease inhibitors (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Roche). Following centrifugation at 16,000 g for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were boiled 15 min at 95°C to gelatinize the starch and hydrolyzed with α-amylase and amyloglucosidase (Roche) to release the glucose blocks ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested either for 1 hour at 37°C in a digestion mix of 3 mg/ml collagenase type A (Roche, 11088793001) and 25 μg/ml DNAse (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Telomeric repeats were probed by incubating the membrane with telomeric probe containing digoxigenin (5’CCCTAACCCTAACCCTAACCCTAA-DIG; Integrated DNA Technologies, Coralville, IA; Table S1) overnight at 42°C DIG Easy Hyb™ buffer (Roche). Blots were washed in 2X saline-sodium citrate (SSC ...
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bioRxiv - Biochemistry 2019Quote: ... and the resulting duplex was captured on 3 mg streptavidin-coated magnetic beads (Roche, rotation for 2 h at 37°C). After extraction with phenol-chloroform and precipitation with ethanol ...
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bioRxiv - Plant Biology 2019Quote: ... The eDNA was stored at −20°C and sent to the Joint Genome Institute for paired-end sequencing using the KAPA-Illumina library creation kit (KAPA Biosystems) and the HiSeq-2500 Illumina platform (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... for overnight at 37°C followed by DNase treatment and purified using Quick Spin Columns for radiolabelled RNA purification (Roche, 11274015001). For Ψ and I modified RNAs synthesis ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Molecular Biology 2021Quote: ... treated with 5.8 μl of proteinase K (24 mg/mL, P4850) for 45 min at 55°C followed by 50 μg/ml RNase A (Roche, 10109169001) for 1 h at 37°C plus 1 h at 65°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then hybridized overnight at 70 °C with a digoxigenin (DIG)-labeled RNA probes (DIG RNA labeling kit, Roche 11277073910) for Gadd45b mRNA ...
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bioRxiv - Cell Biology 2020Quote: ... and AdRb (5′-TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16° C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65° C for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 μL of RNA and anchored oligo(dT)18 primer in a 20 μL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Immunology 2022Quote: ... Kidneys were finely minced and digested for 40 min at 37°C with 0.4 mg/ml collagenase D (Roche, Mannheim, Germany) and 0.01 mg/ml DNase I (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were loaded into 96-well plates and were performed at 63 °C during 35 min using a LightCycler 96 Real-Time System (LC96) (Roche Diagnostics). One melting cycle was performed at 0.1 °C/s from 63 °C up to 97 °C for validation of the specificity of the amplified products ...
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bioRxiv - Immunology 2022Quote: The dorsal halves of the ear skin of mice were collected and incubated for 70 min at 37°C in cRPMI containing 0.33 mg/ml Liberase TL (Roche, Basel, Switzerland) and 0.05% DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... All steps were performed at 4°C and buffers were all supplemented with protease inhibitor cocktail from Roche (cOmplete tablets, EASYpack). The pellets were washed once in buffer A (50 mM Tris/HCl pH8.0 ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Cancer Biology 2022Quote: Engineered monoallelic cell pellets (500 million cells/sample) were lysed at 4°C in 1% CHAPS (Roche Diagnostics, cat no. 10810126001) lysis buffer (pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA probes were in vitro transcribed from the linearized vector for 3 hours at 37°C with the corresponding RNA polymerase and DIG RNA Labeling Mix (Roche #11277073910). The reaction product was verified in 0,8% agarose gel and diluted in hybridization solution for further use ...
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bioRxiv - Cell Biology 2023Quote: ... CDMs were washed with PBS and treated for 1 h at 37°C with a buffer of 50 µg/ml DNaseI (Roche, #11284932001), 5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2023Quote: ... Each brain with known weight was homogenized at 4°C using automated homogenizer and with IX RIPA buffer containing protease inhibitor tablet (Roche, Germany). After that the brain lysates were centrifuged at 12,000 rpm for 10 mins ...
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bioRxiv - Immunology 2023Quote: ... brains and spinal cords were cut into pieces and digested for 30 min at 37 °C with DNase I (Sigma-Aldrich or Roche) at a concentration of 1 mg/ml and collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... and digested at 37°C for an hour with mechanical disruption with a stir bar and enzymatic digestion with collagenase D (Roche 11088875103) and DNase I (Sigma D4527) ...