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Citations for Roche :
2301 - 2350 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the Cell Death Detection Kit (TMR red or FITC; Roche) was used according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... followed by library preparation with the KAPA HyperPrep kit (Roche) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... using the KAPA Stranded mRNA-Seq Kit (KR0960, Roche, USA) and Illumina platforms sample preparation protocol (v3.15 ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were prepared with KAPA SYBR Fast kit (Roche) and run on the Corbett Research Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: ... KAPA SYBR FAST One-Step RT-qPCR kit (Roche, KK4650) was used following manufacturer protocols ...
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bioRxiv - Synthetic Biology 2024Quote: ... using a KAPA HyperPrep PCR-Free kit (Roche, Basel Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Libraries were generated using KAPA Hyper Prep Kit (KAPA Biosystems) and KAPA Real Time Library Amplification Kit (KAPA Biosystems ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted using High Pure RNA Tissue Kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was purified using the RNA purification kit (Roche). qRT-PCR were performed following the manufacturer’s instructions for the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... in conjunction with the ChromoMap DAB detection kit (05266645001; Roche). Lastly ...
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bioRxiv - Cancer Biology 2023Quote: ... with the KAPA hyper prep kit for DNA (now Roche). Truncated universal stub adapters were used for ligation ...
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bioRxiv - Cancer Biology 2023Quote: ... with the KAPA hyper prep kit for RNA (now Roche). Truncated universal stub adapters were used for ligation ...
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bioRxiv - Plant Biology 2023Quote: ... The KAPA SYBR FAST ABI Prism Library Quantification Kit (Roche) was used to quantify the libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... using LightCycler 480 SYBR Green I Master kit (Roche Diagnostics). Calculation of relative expression was determined by the Bio-Rad CFX Manager™ software and fold change was normalised as Normalised Relative Quantity (or ΔΔCq ...
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bioRxiv - Microbiology 2023Quote: ... DNA libraries were prepared using a KAPA HyperPlus kit (Roche). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared using the RNA Hyperprep kit (KAPA Biosystems) and sequencing was performed using an Illumina NextSeq sequencer.
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bioRxiv - Microbiology 2024Quote: ... The KAPA Hyper Prep Kit (KAPA Biosystems, Wilmington, MA, USA) was used to generate sequencing libraries following manufacturer’s instructions using 4 cycles of amplification ...
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bioRxiv - Immunology 2024Quote: ... and quantified using the Kapa Library Quantification Kit (Kapa Biosystems). Each library was sequenced in one lane of HiSeq4000 (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Library concentration was quantified using Kapa Library Quantification kit (Roche). Libraries were pooled for sequencing by Illumina HiSeq 4000 in SR75 mode.
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bioRxiv - Genomics 2024Quote: ... Library concentration was quantified using Kapa Library Quantification kit (Roche). RNA-seq libraries were sequenced with Illumina HiSeq 4000 platform.
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bioRxiv - Cancer Biology 2024Quote: ... using the KAPA HiFi ReadyMix PCR kit (Roche, Basel, Switzerland) with TKO outer forward and reverse primers (76) ...
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bioRxiv - Cell Biology 2024Quote: ... The detection was done using DIG Luminiscent Detection Kit (Roche) following the recommendations of the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... RNA probes were synthesized using DIG-RNA labeling kit (Roche) [70] ...
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bioRxiv - Neuroscience 2024Quote: The In-Situ Cell Death Detection Kit (Roche Life Science) was used to identify apoptotic processes within the brain tissue ...
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bioRxiv - Cancer Biology 2024Quote: ... library was prepared using KAPA HTP library kit (KAPA biosystems). ChIP libraries were sequenced to obtain 30-40 M reads for each sample on HiSeq 2500 or 4000 with either SR50 or PE50.
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using High Pure RNA Isolation Kit (Roche) according to manufacturer’s instructions and quantified with Nanodrop 2000 Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... were quantified using the KAPA Library Quantification Kit (KAPA Biosystems). Pooled libraries were sequenced by an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2 ...
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bioRxiv - Genomics 2020Quote: ... Note that this exome capture kit generates much less discordantly mapped read pairs than the kit from Roche (2 versus 22, compare with Figure 2). Accordingly ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Immunology 2021Quote: ... ATAC-seq libraries were amplified using 5 μl each of the i5 and i7 Nextera Indexing primers and 25 μl of 2x HiFi HotStart ReadyMix (Roche Diagnostics, KK2601). Final libraries were purified using 1x volumes of AMPureXP SPRI-beads and sequence using 75bp paired-end reads on an Illumina NextSeq500.
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... 18.75 mg/ml nitro blue tetrazolium chloride, 9.4 mg/ml 5-bromo-4-chloro-3-indolyl phosphate toluidine salt in 67% dimethyl sulfoxide, Roche; Cat. No. 1681451) was added to the third change of NDB while stirring thoroughly until completely dissolved ...
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bioRxiv - Genomics 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) supplemented with fresh protease inhibitors (AEBSF, Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Emulsions were snap-frozen in droplets in liquid nitrogen and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Neuroscience 2019Quote: ... on wet ice in cold 5 mM Tris-HCL (pH 7.5) with 0.32 M sucrose and protease and phosphatase inhibitor tablets (Complete Mini, EDTA-free and PhosSTOP tablets; Roche Diagnostics, Indianapolis, IN). Protein concentrations were obtained for each homogenate using a BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... from 4L of bacterial culture was resuspended in 30 mL lysis buffer (50 mM Tris-HCl pH 8.3, 1 mM EDTA, 150 mM NaCl, 10% glycerol, 5 mM DTT, 1 mM PMSF, and Roche protease inhibitor cocktail) and sonicated on ice ...
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bioRxiv - Neuroscience 2019Quote: ... BCIP (5-Bromo-4-chloro-3-indolyl-phosphate, 4-toluidene salt, Roche, Cat. No. 11 383 221 001. 105 µl/40 ml) and Levamisole (Vector ...
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bioRxiv - Systems Biology 2019Quote: ... RBCs were then lysed in hypotonic solution (5 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche and PhosSTOP, Roche) with a ratio of 1 volume packed RBC ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were gently resuspended in 25 ml of swelling solution (1 mM PIPES pH 7.0, 1 mM MgCl2, 5 μg/ml taxol and Roche Complete Protease Inhibitors) and immediately centrifuged at 250 g for 3 min ...
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bioRxiv - Plant Biology 2019Quote: ... vortexed in 200 µL ice-cold extraction buffer (20 mM Tris pH 7.5, 300 mM NaCl, 5 mM MgCl2, 0.1% Triton X-100, 1x complete Proteinase-Inhibitor Cocktail from Roche, 50 µM MG-132) and incubated for ten minutes on ice ...
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bioRxiv - Genomics 2021Quote: ... on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol in MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 300 mM MgAc ...
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bioRxiv - Neuroscience 2020Quote: ... the signal was visualized by incubation with nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP) solution (Roche Diagnostics, 11681451001) in 0.1 M Tris-HCl (pH9.5 ...
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bioRxiv - Microbiology 2021Quote: The body weight of all mice was recorded weekly and blood glucose (BG) levels were measured every 5 weeks after fasting for 12 h using a glucose analyzer (Accu-Chek; Roche, Rotkreuz Switzerland) over the tail vein ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Cancer Biology 2021Quote: We established single-cell suspensions of surgically resected tumors by taking a small piece of PDAC tumor tissue collected in a cell-dissociation media [5 ml of minimal essential media (MEM) containing 100μl of Liberase-TM (2.5 mg/ml stock solution Roche/Sigma Aldrich Cat# 5401046001), 50μl of Kolliphor®P 188 (15 mM stock solution Sigma Cat# K4894) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellets were lysed in 200μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 5 mM MgAc, 2% digitonin, and 1X Roche protease inhibitor cocktail) by incubating on ice for 30 min and then diluted to 1ml with the lysis buffer containing 0.1% digitonin ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2% NP-40, 10% glycerol, 1 mM EDTA, 1 mM PMSF, 20 µM MG132, 5 mM DTT and Roche protease inhibitor #5892953001), and incubated for 1 h on a rotating wheel ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) in the presence of protease inhibitors (Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Suspensions were flash frozen in droplets and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...