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Citations for Roche :
2301 - 2350 of 3087 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... gene expression (5’Gex) and TCR libraries were generated then quantified via Agilent Technologies 4200 Tapestation and KAPA library quantification kit (Roche). Targeted read depth included 25,000 reads per cell for gene expression libraries and 5,000 reads per cell for TCR libraries ...
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bioRxiv - Developmental Biology 2024Quote: ... knee cartilage was harvested from postnatal day 3-5 mice and treated with 3 mg/ml collagenase D (11088866001, Roche) in DMEM (10569010 ...
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bioRxiv - Bioengineering 2024Quote: ... in PBS (PBST) and then blocked for 3 hours at RT in PBS with 5 wt% bovine serum albumin (BSA, Roche), 5% goat serum (Gibco) ...
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Ion channel function of polycystin-2/polycystin-1 heteromer revealed by structure-guided mutagenesisbioRxiv - Biophysics 2024Quote: ... Oocytes were subsequently transferred into 1 mL lysis buffer (Tris 25mM, NaCl 150 mM, EDTA 1mM, NP40 1%, Glycerol 5%, supplemented with Roche cOmplete™ protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2024Quote: Cells were resuspended in cold lysis buffer (50 mM Hepes pH 8, 50 mM NaCl, 5 mM MgCl2 and 1 tablet 50 mL−1 EDTA-free protease inhibitor, Roche) plus 0.5 mg/mL lysozyme and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were then washed three times for 5 min with TBST and developed using the Lumi-Light Western Blotting Substrate (Hoffmann-La Roche). The chemiluminescence signal was detected using a Fusion FX6 reader (Vilber ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... The buffer was aspirated and minced tissue was digested in 5 ml cold HBSS adding 100 μl 10mg/ml Collagenase P (Roche) for 15-20 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Suspended membrane vesicles were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... slices were blocked for 30 min (2% BSA, 0.1ug/ul Salmon Sperm, 0.5% Saponin, 1 U/µl protector RNase inhibitor (Roche) in 3X SSC ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of DNA were used per reaction with the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... rinsed in TBS/0.1% Tween-20 and incubated overnight in blocking buffer (TBS with 2% goat serum, 0.1% blocking reagent (Roche), 0.1% Tween-20 ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche] ...
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bioRxiv - Neuroscience 2021Quote: ... The floating sections were then treated with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml, 10236276001; Roche Diagnostics) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM MOPS, pH 7.2; 2 mM EDTA; 1 mM PMSF; 1X cØmplete, mini, EDTA-free protease inhibitor cocktail, Roche). Cells were lysed by bead beating with 0.5 mm glass beads for 3×20s at 6.5 m/s in a benchtop homogenizer (Fastprep-24 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM MOPS, pH 7.2; 2 mM EDTA; 1 mM PMSF; 1X cØmplete, mini, EDTA-free protease inhibitor cocktail, Roche). Cells were lysed by bead beating with 0.5 mm glass beads for 3×20s at 6.5 m/s in a benchtop homogenizer (Fastprep-24 ...
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bioRxiv - Neuroscience 2020Quote: ... 40 mM HEPES, pH 7.4, 2 mM EDTA, 10 mM pyrophosphate, 10 mM glycerophosphate, 0.3% CHAPS, protease inhibitors from Roche) as indicated.
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bioRxiv - Molecular Biology 2020Quote: Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, Mannheim, Germany), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Patient cfDNA was isolated from 2 mL of plasma using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 genomic RNA was isolated from cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems, K4602) using specific primers for β-actin ...
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bioRxiv - Cancer Biology 2021Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (11465007001, Roche Diagnostics, Mannheim, Germany) was performed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Afterwards samples were incubated for 2 h in the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) enzyme labeling solution at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 M PIPES-NaOH pH 6.9, 2 mM EGTA, 1 mM MgSO4, 0.1 mM EDTA, cOmplete protease inhibitors [Roche]) (5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested for 30 minutes at 37 °C in DMEM + 2 mg/mL Collagenase A (Roche, Basel, Switzerland) + DNase (Roche ...
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bioRxiv - Immunology 2020Quote: ... patients with at least one nasopharyngeal swab positive for SARS-CoV-2 as determined by qRT-PCR (Roche LightCycler480 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1 × protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 μl/10 mg) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were resuspended in Lysis buffer (50 mM Tris pH 8.0, 300 mM NaCl, 2 mM DTT, 0.3 % TritonX-100, Roche Complete protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2021Quote: Lysis buffer 2x (200 mM Tris, 300 mM NaCl, 2% triton, pH 7.4) and Complete proteinase inhibitor (Roche) were added to homogenized organs for 30 min on ice ...
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bioRxiv - Genomics 2021Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were incubated in 2 ml HBSS solution containing 0.21 mg/ml Liberase TL (Roche; cat: 5401020001) or DH (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Immunology 2021Quote: ... The Roche Elecsys anti-SARS-CoV-2 assay was performed on Roche Cobas e411 (Roche Diagnostics, Indianapolis, IN). The Elecsys antinSARSnCoV-2 assay uses a recombinant protein representing the N antigen for the determination of antibodies against SARSnCoVn2 ...
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bioRxiv - Biochemistry 2021Quote: ... 150 mM NaCl and 1 mM MgCl2) containing protease inhibitors (2 tablets EDTA-free protease inhibitor cocktail (Roche), 2.6 mg/L aprotinin ...
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bioRxiv - Biophysics 2021Quote: ... Isolated membrane fractions were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.0, 20 mM KCl, 1 mM MgCl, 2 mM EDTA, pH 7.5 and protease inhibitor cocktail, Roche) from cells grown in EMM with appropriate glucose concentrations and with or without thiamine (5μg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... extremities and organs were removed from E13.5 embryos and the remainings were enzymatically digested with Blendzyme 2 (0,5 mg/ml; Roche Applied Sciences ...
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bioRxiv - Microbiology 2021Quote: The cytotoxicity assays were performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Roche, ref:11465007001) protocols according to the previous study [47] with few modifications ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of saliva sample was mixed with 2 µL of 10 U/µL α2-3,6,8-Neuraminidase (Sialidase) from Arthrobacter ureafaciens (Roche) and reacted at 37°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Pellet was resuspended in 2 mL of homogenization buffer (250mM sucrose, 10mM HEPES, 1mM EDTA, protease inhibitors (Roche), 1mM PMSF ...
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bioRxiv - Developmental Biology 2020Quote: ... and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: ... pH 7.2) with 2 mM PMSF and EDTA-free Complete Protease Inhibitors Cocktail tablets (Roche Diagnostics, Mannheim, Germany), and its supernatant was transferred onto 30-60% sucrose gradient ...
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bioRxiv - Genomics 2020Quote: ... Specimens in May were tested using the Cobas® SARS-CoV-2 RT-PCR assay (Roche Diagnostics, USA). Respiratory samples archived at the Clinical Laboratory ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were removed and pellets were resuspended in 2% BSA/PBS containing RNase inhibitor (0.2 U/μL, Roche). To enrich astrocyte nuclei ...
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bioRxiv - Biochemistry 2022Quote: ... 500 mM NaCl, 2 mM TCEP, 20% glycerol, 1.4 μg/mL leupeptin, 1.0 μg/mL pepstatin A and two Roche EDTA-free protease tablet per 5 g cell pellet) ...
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bioRxiv - Biochemistry 2022Quote: ... 500 mM NaCl, 2 mM TCEP, 20% glycerol, 1.4 μg/mL leupeptin, 1.0 μg/mL pepstatin A and two Roche EDTA-free protease tablet per 5 g cell pellet) ...