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Citations for Roche :
2251 - 2300 of 10000+ citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 1 WFIKKN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Proteins were extracted using EB2 supplemented with 1X PhosSTOP (Roche, Indianapolis, IN) and FLAG immunoprecipitation was performed using anti-FLAG M2 agarose (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were immunoprecipitated using a monoclonal anti-GFP antibody (Roche). Analysis of enrichment of target genes was performed by qPCR using the oligos listed in the Table S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were digested with 0.04 mg/mL proteinase K (Roche #03115852001). DNA was recovered using a DNA purification kit (Bioline #52060) ...
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bioRxiv - Neuroscience 2022Quote: ... protein G-Sepharose Fast Flow and immobilized streptavidin Mutein Matrix from Roche; protein molecular weight standards from Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... After last wash proteins were cleaved by adding sequencing grade trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Cell Biology 2023Quote: ... Fusion proteins were detected with α-Myc monoclonal antibodies (Roche, Stockholm, Sweden). The following constructs were used ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μl of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μl of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μL of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then degraded by addition of 50 µg Proteinase K (Roche) and incubation at 56°C for 90 minutes ...
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bioRxiv - Cell Biology 2023Quote: The TetR-eYFP tagged proteins were transfected using the XtremeGene-9 (Roche) transfection reagent according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and subsequently incubated with 20 μL of protein G-agarose beads (Roche) or protein A-agarose beads (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellets were suspended in in cOmplete Protease Inhibitor Cocktail (Roche) containing radio-immunoprecipitation assay (RIPA ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA of 1 μg from each sample was reverse transcribed with random hexamers using Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... by the capillary method and hybridised with digoxigenin-labelled blaOXA- 58 and blaNDM-1-specific probes with an NBT/BCIP colour detection kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 µg RNA was used for cDNA synthesis with oligo(dT)12-18 using Transcriptor first-strand synthesis kit (Roche, Basel, Switzerland). Transcript levels were measured by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Evolutionary Biology 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared using >1 μg of pooled DNA with a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Exome libraries were prepared from 1 μg of genomic DNA from each analyzed section using the Nimblegen EZ Exome kit V3 (Roche, Nutley, NJ). Paired-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed with lysis buffer (500 μL) containing the cOmplete protease inhibitor cocktail (Roche, cat#11697498001). The biotinylated surface proteins were excluded with NeutrAvidin agarose gel (Pierce ...
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Microbiology 2020Quote: Square plates containing standard Middelbrook 7H10 OADC agar were prepared and supplemented with kanamycin (Roche,20ug/ml), with or without anhydrotetracycline (ATc ...
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bioRxiv - Genomics 2022Quote: ... a PCR was performed with primers containing NGS sequences (KAPA HiFi HotStart ReadyMix, Kapa Biosystems, 12 cycles), and the library was cleaned using 0.8x SPRI beads.
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bioRxiv - Immunology 2021Quote: ... PCR was performed in 25 µl reaction mix containing 2x KAPA HIFI HotStart Ready-mix (Roche #07958935001), 10µM primers and 10 ng of gDNA template from stool or tissue under the following conditions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and 5 mM ethylenediaminetetraacetic acid (pH 8.0)] containing protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN, USA) for 45 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... in a 20 µL reaction containing 10 µL LightCycler ® 480 SYBR Green I Master Mix (Roche) and 0.8 µL each of 10 µM forward and reverse primer listed in Supplementary Table S2 ...
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bioRxiv - Genomics 2019Quote: ... We then added 15 µl PCR master mix containing 12.5 µl KAPA HiFi HotStart ReadyMix (KAPA Biosystems)) and 0.5 µl 10 µM PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT) ...
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bioRxiv - Systems Biology 2019Quote: ... pH 7.4) containing protease and phosphatase inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche and PhosSTOP, Roche) with a ratio of 1 volume packed RBC ...
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bioRxiv - Immunology 2021Quote: ... and then dissociated with gentleMACS dissociator (Mylteni) and dissociated in RPMI containing 0.167mg/mL liberase TL (Roche), 0.25mg/mL DNase I (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed in 25 μl reactions containing 12.5 μl KAPA HiFi Hotstart ReadyMix [2X] (Kapa biosystems), 1.25 μl 5 μM mPing fusion primer (Supplemental Table 3) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in AYE media containing a complete mini EDTA-free protease inhibitor cocktail tablet (Roche) and adjusted to an OD600 of 20 ...
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bioRxiv - Neuroscience 2020Quote: ... EDL muscle samples were thawed on ice and supplied with RIPA buffer containing 0.05% protease inhibitors (Roche) and 0.3% 1M DTT (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml) (Roche). Damaged livers (48h-72h after APAP injection ...
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bioRxiv - Cell Biology 2021Quote: Cells were re-suspended in lysis buffer containing 2x concentration of PIC (Roche complete, EDTA-free #05056489001) and 2 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0 containing 10% glycerol (v/v) and the Complete Mini protease inhibitor cocktail (Roche, Mannheim, Germany) for 18 h at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... The cell pellets were then lysed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo) ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended and washed in carbonate solution (0.1M Na2CO3, pH 11.0) containing protease inhibitors (Roche), and after incubation for 30 min at 4°C the sample was centrifuged again at 100,000 g for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Muscles were dissociated and digested in RPMI medium containing 0,2% of collagenase B (Roche Diagnostics GmbH 11088815001) at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... The tissues were homogenized using a hand-held homogenizer in RIPA buffer containing protease inhibitor cocktail (Roche) and centrifuged at 4 °C to remove cell debris ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 mM imidazole) containing 200 µg/mL lysozyme and an EDTA-free protease inhibitor cocktail (Roche Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... Ph 7.5 containing 5mM ß-mercaptoethanol and proteases inhibitors (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Roche). Cell debris were removed by centrifugation (10 min ...
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bioRxiv - Immunology 2020Quote: ... brains were cut into 6 pieces and incubated in 5 mL HBSS containing 50U/mL DNase (Roche), and 4U/mL papain (Worthington-Biochem ...
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bioRxiv - Immunology 2020Quote: ... harvested back skin was minced with scissors in RPMI medium containing 0.1 mg/ml DNase I (Roche) and 1mg/ml Collagenase I (GIBCO) ...
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bioRxiv - Immunology 2020Quote: ... Brains were subsequently transferred to a new tube containing 1mL RPMI with Collagenase I/DNAse I (Roche) and cut to smaller pieces ...
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bioRxiv - Developmental Biology 2022Quote: ... The embryos were then transferred to TBST containing 10% HISS and anti-DIG-AP antibody (Roche, 11093274910) at a dilution of 1:2000 overnight at 4C with constant rocking ...