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Citations for Roche :
2251 - 2300 of 3048 citations for 6 Chloro 9 2 C methyl β D riboFuranosyl 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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β-amyloid−driven synaptic depression requires PDZ protein interaction at AMPA-receptor subunit GluA3bioRxiv - Neuroscience 2021Quote: ... 150 mM NaCl) containing 2% Triton X-100 and EDTA-free protease inhibitor cocktail (Roche). The resulting samples were incubated for 1 h at 4°C and spun down twice at 20,800 x g for 10 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured in complete media with 30 IU/mL IL-2 (Roche, 202-IL). Media containing IL-2 was replenished daily for 10 days ...
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bioRxiv - Microbiology 2022Quote: ... one of which was treated with 2 U of PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 1 hour ...
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bioRxiv - Physiology 2022Quote: ... pyruvate supplemented with 2% Bovine Serum Albumin Fraction V Fatty acid free (Roche, USA/UK), 50 µM 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μL of 10x PCR Reaction Buffer with MgCl2 (Roche Life Science, Indianapolis, IN, USA), 0.15 μL Taq DNA Polymerase (Roche Life Science ...
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bioRxiv - Cancer Biology 2019Quote: ... NK-92 cultures were supplemented with 150 IU/ml rhIL-2 (Hoffman-La Roche Inc.). The amphotropic-packaging 293T and Phoenix cells were cultured in Dulbecco modified Eagle medium (DMEM) ...
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bioRxiv - Cell Biology 2020Quote: ... 9ml of cold wash buffer (PBS, BSA 2% and 0.2U/μl RNase inhibitor from Roche) was added and the lysate was dounced with 10 strokes of loose pestle avoiding too much pressure and air bubbles ...
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bioRxiv - Biochemistry 2019Quote: ... 20% glycerol) containing 0.5 μM 2-mercaptethanol (Fujifilm Wako) and protease inhibitor (Roche, Basel, Switzerland) and solubilized with 0.6% (w/v ...
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bioRxiv - Physiology 2019Quote: ... Glomus cells were dissociated using a mixture of collagenase P (2 mg/ml; Roche, USA), DNase (15μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... mRNA expression analysis was determined using KAPA SYBR Fast Master Mix (2×) Universal (KAPA Biosystems) in an Eco Real-Time PCR System (Illumina) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2% Triton X-100 and 274 mM NaCl) containing a cocktail of protease inhibitors (Roche). Cells were lysed for 20 min on ice and centrifuged for 15 min at 17,400 r.p.m ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001) and thermal cycler program ...
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bioRxiv - Biophysics 2020Quote: ... 5 mM MgCl2 (buffer B) containing 2 mM PMSF and Complete protease inhibitor cocktail (Roche). After centrifugation ...
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bioRxiv - Genetics 2021Quote: ... 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche) was used to resuspend the cells ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were counterstained with 5 ng/ml of 4,6–diamidino-2-phenylindole dihydrochloride (DAPI; Roche). Coverslips were mounted on slides with Fluoromount G (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2020Quote: ... diluted 2-fold with PBMC culture media and supplemented with 10% WST-1 reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and the RNA was subsequently precipitated in 2-propanol and 20 μg/μl glycogen (Roche) overnight at −20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... femurs were cut roughly and incubated with 2 Wunsch units of LiberaseTM (Sigma/Roche 5401127001) and 1 mg of Pronase (Sigma/Roche 10165921001 ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM PMSF) supplemented with 1 protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM beta-mercaptoethanol) in the presence of a protease inhibitor cocktail (Roche, Basel, Switzerland). These mixtures were incubated on ice for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... was amplified using F3 and R3 primers (Supp. Table 2) and KAPA-Hifi polymerase (Roche). Following a 1X SPRIselect bead DNA cleanup (Beckman Coulter) ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were incubated with 2% Dispase II (neutral protease; Roche Applied Science, Indianapolis, IN) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Immunology 2024Quote: ... 2 mL aliquots of peripheral blood were treated with 30mL of RBC lysis buffer (Roche) for 5-8 minutes at room temperature with gentle mixing ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalogue #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM PMSF) supplemented with one protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Immunology 2023Quote: Anti-nucleocapsid IgG was measured using the Elecsys Anti-SARS-COV-2 assay (Roche; 09203095190) run on a Cobas e411 analyser (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 2 ng of cDNA was amplified per reaction in a 364-well plate (4729749001, Roche) with LightCycler® 480 SYBR Green I Master (4887352001 ...
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bioRxiv - Developmental Biology 2023Quote: ... was diluted 1 in 50 in solution consisting of 2 % blocking reagent (Roche ref 11096176001), 1 % goat serum (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM TCEP and 5 % glycerol) supplemented with cOmplete EDTA-free protease inhibitor complex (Roche) and ribonuclease A (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μg/mL DAPI (4,6-diamidino-2-phenylindole, F. Hoffmann-La Roche, Natley, NJ, USA) was added to counterstain the nuclei ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then digested with Liberase Blendzyme 2 (0.2 mg/ml, Roche Applied Science) and DNase I (20 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated in complete RPMI-10 supplemented with 50U/mL hIL-2 (Roche 11011456001), 2.5ng/mL TGFβ (R&D 240-B-10) ...
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bioRxiv - Microbiology 2024Quote: ... 25 wt% sucrose and 2 mg/mL lysozyme (from egg white, 10837059001 Roche, Basel, Switzerland) and incubated for 30 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml; Roche, Switzerland) for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were dissociated by incubation in DPBS (without Mg2+ or Ca2+) for 20 min at 37°C and then in 1:200 Liberase TH (Roche, Basel, Switzerland) in DPBS for 20 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Bound proteins were eluted with a 30 minute incubation at 4°C with Elution Buffer (50 mM Tris pH 8.0, 40 mM reduced glutathione, and Roche Complete protease inhibitors). At the end of the incubation ...
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bioRxiv - Cell Biology 2019Quote: ... cells were harvested through centrifugation at 5000 rpm for 10 min at 4°C and afterwards washed with 2mL PBS supplemented with 1x protease inhibitors cocktail (Roche, Basel, Switzerland). Cell pellet was resuspended in lysis buffer (150 mM KCl ...
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bioRxiv - Neuroscience 2019Quote: ... 30μm thaw-mounted sections were hybridized overnight at 62°C with a DIG-labelled riboprobe for TVAG or HM3 (approximately 1:500; Roche, Cat. 11277073910) and then incubated in Blocking solution (600µl MABT ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then incubated for 1 h at 4° C in PBS + 1% BSA with a 1:1000 dilution of rat anti-HA monoclonal antibody (Roche; Basel, Switzerland) and washed 5×10 min on ice with PBS + 1% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then stained overnight at 4°C with pre-absorbed AP-conjugated sheep anti-DIG antibody (1:1000, Roche, cat. 11093274910) in PBS containing 0.1% Triton X-100 and 20% horse serum ...
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bioRxiv - Plant Biology 2021Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 10,000 RPM for 30 minutes at 4°C and resuspended in 40mL lysis buffer (20mM HEPES, pH 7.4, 1M NaCl, 1mM EDTA, 1mM DTT, Roche protease inhibitor cocktail). Lysozyme was added at a final concentration of 1mg/ml and cells were incubated on ice for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... then incubated with primary antibody at 4°C overnight in Wash Buffer (10 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) with 2mM EDTA ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse MEC organoids were prepared by manual chopping of mouse mammary gland tissues harvested from 10–12-week-old mice followed by digestion with shaking for 1 hr at 37 °C with Collagenase A from Clostridium histolyticum (10 mg/mL; Roche, Cat. #: 13560925) and Hyaluronidase from bovine testes (1 mg/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quenched PFA solution was then removed and the tissue was resuspended in ice-cold Hi-C Lysis Buffer (10mM pH=8 Tris-HCl, 10mM NaCl, 0.2% NP-40 and 1x Roche Complete protease inhibitor). The lysis was helped with a Dounce Homogenizer Pestle A on ice (series of 10 strokes in 10’ intervals) ...
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bioRxiv - Microbiology 2021Quote: Bacteria-captured beads were incubated at 37°C for 1 hour with 10 mg/mL of lysozyme (Cat # 10837059001, Roche, Basel, Switzerland) diluted in 10 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were minced and then digested at 37°C for 20 min with an enzyme cocktail (collagenase A, dispase and DNaseI, Roche Applied Science). Single-cell suspensions were prepared by filtering through a 70-μm strainer and passing through a nylon mesh ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...