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Citations for Roche :
2201 - 2250 of 4072 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed using ApopTag Red In Situ Apoptosis Detection Kit (S7100, MerckMillipore) combined with peroxidase (POD) coupled anti-DIG antibody (1/200, #11207733910, Roche) followed by tyramide-based amplification61.
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bioRxiv - Cell Biology 2022Quote: ... Blotting was done as described in (Konrad et al. 2010) using primary rat-derived anti-HA antibody (dilution 1:500, Roche) followed by anti-rat (dilution 1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at +4 °C with the antibody anti-digoxigenin-AP Fab fragment (#11093274910, Roche, 1:1000) diluted in blocking solution in a humidity chamber ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then incubated overnight at 4 °C with primary antibody of interest diluted in blocking solution (Western Blotting Reagent, Roche). The appropriate species of HRP-conjugated secondary antibody (from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with primary antibody for rat anti-HA (1:200; 12013819001, RRID:AB_390917, Roche, Basel, Switzerland), rabbit anti-GFP (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with α-DIG or α-fluorescein antibody conjugated with alkaline phosphatase (Roche, 1:2000 in 10% FBS/PBST) at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: The Fab fragment of antibody CR3022 was prepared by digestion of the full-length IgG using enzyme Lys-C (Roche). The digestion reaction was allowed to proceed for 2.5 hours at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated overnight at room temperature with anti-DIG antibody conjugated with the alkaline phosphatase (1/5,000, Roche Diagnostics) in B1 containing 1% NGS ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Zoology 2022Quote: ... The DIG-labeled hsd3b probe was visualized by using a horseradish peroxidase-conjugated anti-DIG antibody (Roche Diagnostics, Basel, Switzerland) and TSA Plus Cy3 System (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000; Roche Applied Sciences). Sections were washed thrice with PTW buffer and twice with coloration buffer (100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... cells were in contact for 2 hours at room temperature with a mouse monoclonal primary antibody anti-BrdU (1/1000 dilution) (Roche), then rinsed and incubated for 30 min with a fluorescent secondary antibody Alexa-633nm goat anti-mouse (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against rat anti-HA clone 3F10 (Roche) or rabbit anti-TgSKP1 UOK75 [19]
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bioRxiv - Synthetic Biology 2019Quote: ... or NF-YC3:FLAG was probed with high affinity anti-HA primary antibodies (Roche, catalog no. 11 867 423 001), anti-MYC (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... for 1 hr and incubated overnight with sheep anti-DIG-POD primary antibody (1:1000, for the DIG-labeled probe; Roche) or mouse anti-biotin (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Biochemistry 2019Quote: ... The antibodies used for the brain-ring gland complex included a rat anti-HA high-affinity monoclonal antibody (3F10, 1:20 dilution; Roche), a guinea pig anti-Shroud antibody (67 ...
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bioRxiv - Genomics 2020Quote: ... They were then incubated with TNB (5% FBS in TNT) for 15 min before incubation with peroxidase-conjugated anti-DIG antibody (DIG-POD, Roche) 1:200 in TNB for 2 hr at RT ...
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bioRxiv - Pathology 2020Quote: ... Tween-20 0.1%) for 1 hour and incubated overnight at 4 °C with the appropriated antibodies: HA (dilution 1:1000, Roche, #867423001), ß-tubulin (dilution 1:5000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1:4000 in the blocking solution ...
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bioRxiv - Developmental Biology 2020Quote: ... and blocked in 10% Sheep Serum for 2 hours followed by incubation with an anti-DIG antibody (1:2000) (Roche) in TBST / 1% sheep serum overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections and antibody staining were performed as follows: two micrograms plasmid DNA was transfected using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Incubation with the appropriate secondary HRP-labeled antibody was followed by detection with ECL western blotting substrate (Roche Applied Science).
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bioRxiv - Physiology 2020Quote: ... Sections were then dried and covered in an alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:3000; Roche, Mannheim, Germany) in buffer B1/2.5% goat serum at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked in 10% inactivated sheep serum for 1 h followed by overnight incubation with 1:1000 anti-digoxygenin (DIG) antibody (Roche). The sections were washed in PBT and incubated with NBT/BCIP (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... a linear DNA tether of the same length was amplified using a digoxigenin-modified primer instead of NTA (5’-DIG-NHS-TCCAAAGGTGAAGAACTGTTCACC, Integrated DNA Technologies, Inc. USA) to bind to anti-digoxigenin Fab fragment antibodies (11214667001, Roche).
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bioRxiv - Cancer Biology 2021Quote: ... 12 minutes incubation with the HRP-conjugated secondary antibody (DISC. Omnimap anti-Ms HRP RUO or DISC. Omnimap anti-Rb HRP RUO, Roche) and 16 minute incubation with the Opal reactive fluorophore (Akoya Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... They were incubated 1h at RT in a blocking buffer (20% Blocking reagent + 20% Goat serum) and then overnight with an anti-DIG-AP antibody (1:2000, Roche) in the blocking buffer ...
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bioRxiv - Genomics 2020Quote: ... Sections were then washed several times with SSC of increasing stringency and then incubated with alkaline phosphatase-conjugated anti-digoxigenin antibodies (Roche). Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated overnight at 4°C with an anti-DIG-AP antibody (Roche, diluted 1:2000 in blocking solution). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with blocking solution for 1 hour and finally incubated overnight at 4°C with anti-Fluorescein-POD antibody (Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... the slides were washed in TBS/T 3 times and then incubated with secondary antibodies (2 µg/ml) and DAPI (1 µg/ml, Roche) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were mixed with the beads for 10 minutes following resuspension in 50uL Antibody buffer (20mM HEPES, 150mM NaCl, 0.5mM Spermidine, 0.025% Digitonin, 2mM EDTA, Roche Complete Inhibitor). M2 FLAG antibody was added to a final dilution of 1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor and tumoroid sections were in-cubated for 40 min with the appropriate antibody before incubation with Discovery UltraMap anti-Rabbit (760–4315, Roche) or anti-mouse horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:500 for 32 min was used followed with the secondary antibody OmniMap anti-rabbit HRP (760-4311, Roche). Antigen-antibody complexes were revealed with ChromoMap DAB Kit (Roche ...
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bioRxiv - Genetics 2022Quote: ... MYC-tagged or FLAG-tagged recombinant proteins were captured using ~5μg antibody and Protein-Agarose beads (Roche Applied Science, 11243233001) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... Sections after the post-hybridization washing and blocking steps were incubated with peroxidase-conjugated anti-digoxigenin sheep antibody (1:1000; 11207733910, Roche) in TS 7.5 containing 1% Blocking Reagent at 4°C for overnight ...
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bioRxiv - Physiology 2022Quote: ... the sections were blocked in TS7.5 containing 1% Blocking Reagent for 1 hr and then incubated with alkaline phosphatase-conjugated anti-digoxigenin sheep antibody (1:1000; 11093274910, Roche) and anti-EP3R rabbit antibody in this blocking solution overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Hybridised probes were detected with anti-DIG antibodies and revealed with alkaline phosphatase-conjugated antibodies in presence of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
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bioRxiv - Neuroscience 2022Quote: ... brains were incubated with primary antibodies (1:200, chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913 ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization signals were detected with anti-mouse secondary antibody labeled with alkaline phosphatase using NBT/BCIP substrates (Roche Biochemical, Mannheim).
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bioRxiv - Microbiology 2022Quote: ... and visualized with anti-DIG-AP antibody according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) instructions on a Chemidoc MP.
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bioRxiv - Molecular Biology 2022Quote: ... Sonicated samples were centrifuged and the supernatant (soluble fraction) was incubated for 1 h at 4°C with beads crosslinked to anti-GFP antibody (11814460001; Roche) or to M2 anti-FLAG antibody (F1804 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked in 5% non-fat milk in TBST for 1 h and immunoblotted with an antibody against HA directly conjugated to HRP (anti-HA-HRP; Roche) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked for 30 min and then incubated in an alkaline phosphatase conjugated anti-DIG antibody (1:600, Roche). Slides were then washed and developed overnight in BCIP/NBT chromogen (Perkin Elmer) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibody labelling (16-120 min) and signal detection carried out with the OptiView DAB IHC Detection Kit (VENTANA/Roche), as described previously (33) ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...