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Citations for Roche :
2101 - 2150 of 2800 citations for Archaemetzincin 2 AMZ2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The cells were pelleted by centrifugation (750 x g, 2 min, 4 °C) and resuspended in 50 μl vPBS containing EDTA-free protease inhibitor (Roche). The cells were fixed by addition of 0.5 ml ice-cold fixation solution (4% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Table S2) and measured with a LightCyclerTM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels.
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bioRxiv - Molecular Biology 2023Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 mL dissociation buffer (RPMI-1640 (Cytiva) with 25 mM HEPES and 10 µg/mL gentamicin sulfate) supplemented with 2 mg/mL Collagenase-D (Roche) and 100 U DNaseI (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Neuroscience 2023Quote: Hippocampi were homogenized in 500µl of RIPA medium lysis buffer (Beyotime, #P0013E-2) in the presence of protease inhibitor cocktail (Roche, #11836170001). Samples were centrifuged at 10,000 × g for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Molecular Biology 2023Quote: ... uteri were harvested from pregnant mice at 4.5 days post coitus and washed with cold swelling buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 1X Protease Inhibitor Cocktail (PIC, Roche, 11836170001)) immediately after collection ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Microbiology 2024Quote: ... and lysed by incubation with nondenaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl [pH 7.4], 2% Triton X-100, and 1×EDTA-free protease inhibitor cocktail [Roche Complete]) for 30 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% Cholesteryl Hemissucinate (CHS) (Avanti Pola lipids) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl and 0.5% SMALP200 (CubeBiotech GmbH) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Immunology 2024Quote: ... digested three times (DMEM high glucose with 2% FBS, 100 µg/ml DNase I, and 100 µg/ml Liberase TM, Roche) or until no tissue fragments remained ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-peroxidase antibodies (1:500, Roche) and revealed using the TSA-Plus Cyanine-3 kit (Perkin-Elmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
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bioRxiv - Neuroscience 2019Quote: ... the DIG-labeled probe was visualized by using alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics) and Fast Red (Roche Diagnostics) ...
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bioRxiv - Physiology 2019Quote: ... For Western blots and immunoprecipitation experiments the following primary antibodies were used: anti-HA (Roche, 11867423001), anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... The FLUC-HA and NLUC-HA proteins were detected with anti HA-HRP antibody (Roche 3F10) (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with anti-HA-peroxidase antibody (1:5,000; Roche, Cat# 12013819001, clone 3F10) for 1 h at room temperature followed by washing with PBS-T for three times ...
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bioRxiv - Plant Biology 2019Quote: ... Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001, Roche) or anti-actin 1:2,000 (A0480 ...
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bioRxiv - Cell Biology 2019Quote: ... Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche), anti-cMYC antibody 9E10 (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and hybridized to a mouse monoclonal antibody against the P16 protein (Ventana Roche Diagnostics, E6H4, Switzerland) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... HA-Kir6.2 was detected with HRP-conjugated rat anti-HA antibody (Roche, 3F10, 0.005 μg/ml). GFP was detected with mouse anti-GFP antibodies (AbCam ...
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bioRxiv - Evolutionary Biology 2021Quote: ... labelled riboprobes were detected using anti-digoxigenin or anti-fluorescein antibodies conjugated to horseradish peroxidase (Roche). Fluorescent tagging was performed using Alexa Fluor 594 and 488 dyes with streptavidin Tyramide Signal Amplification (TSA ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with 1:2000 dilution of anti-DIG-alkaline phosphatase-coupled antibody (Roche, Switzerland) in 10% sheep serum overnight ...
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bioRxiv - Biophysics 2021Quote: ... The following primary antibodies were used at the indicated dilutions: mouse anti-Myc (Roche, 1:3000), goat anti-p75NTR (Neuromics ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:1000, 11093274910; Roche) at 4°C for two days ...
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bioRxiv - Neuroscience 2021Quote: ... Post hoc immunostaining was performed using rat anti-HA primary antibodies (Roche, #11 867 423 001) and anti-rat Alexa568 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-GFP (1:2’000; Roche), anti-HA (clone 16B12 ...
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bioRxiv - Neuroscience 2019Quote: ... Dioxygenin (DIG)-labeled probes were detected using peroxidase-conjugated anti-DIG antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using fluorescein-tyramide conjugate ...
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bioRxiv - Cell Biology 2021Quote: Commercially available primary antibodies used for western blot are rat anti-HA tag (clone 3F10; Roche), rabbit anti-Cyclin B1 (4138S ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used in this study were: rat anti-HA (1:250; Roche 3F10, RRID: AB_2314622), rabbit anti-cleaved Caspase-3 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Dilutions for primary antibodies were: mouse monoclonal (mixture of clones 7.1 and 13.1) anti-GFP (Roche), 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... the slides were incubated for 5 h at 8°C with mouse anti-DIG antibody (Roche, Basel ...
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bioRxiv - Microbiology 2019Quote: ... GRA5 blots were subsequently incubated with an anti-mouse secondary horseradish peroxidase (HRP)-conjugated antibody (Roche). The GRA7 blot was incubated with a secondary antibody (HRP)-conjugated anti-rabbit antibody (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... Dig was detected using anti-Dig antibody conjugated with alkali phosphatase and CDP-Star substrate (Roche) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the southern blot was detected by using an anti-digoxygenin antibody coupled to alkaline phosphatase (Roche) and the labeling and detection starter kit (Roche).
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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bioRxiv - Developmental Biology 2022Quote: The following primary antibodies were used in this study: anti-HA (rat, 1:200, 11867423001, Roche), anti-V5 (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analysed by western blotting using a α-GFP antibody (Roche, 11 814 460 001), and a α-Pgk1 antibody (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated for 1h in 1% milk prepared in TBST with anti-GFP antibody (Roche). The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad) ...