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Citations for Roche :
2101 - 2150 of 2345 citations for 2 Methoxycarbonyl 4 methylthiophene 3 diazonium tetrafluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: cDNA was generated from TRAP-isolated and total input RNA samples (n=2 samples/group) using the Transcriptor First Strand cDNA Synthesis Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice in cold 1× PBS and harvested in 2% SDS in 50 mM HEPES buffer with freshly added protease and phosphatase inhibitors (Roche). Cell lysates were sonicated and centrifuged for 15 min at 12000 ×g to remove the insoluble fraction ...
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bioRxiv - Molecular Biology 2022Quote: ... 300 mM NaCl, 20% glycerol, 2 mM MgCl2, 0.2 mM EDTA, 0.1% NP40, 0.5 mM DTT and 1x Roche protease inhibitor cocktail) on a rotating wheel at 4° C for 1 h in triplicates with GFP-Trap agarose beads (#gta-200 ...
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bioRxiv - Microbiology 2022Quote: ... and lysed by incubation for 30 min on ice in non-denaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl, pH 7.4, 2% Triton X-100, and 1x EDTA-free protease inhibitor cocktail [Roche Complete]). For immunoblot assays ...
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bioRxiv - Microbiology 2022Quote: ... GTP-binding Irgb6-T95D crystals diffracting to 1.68 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution containing 2 mM GTP (Roche) and a 0.5 μl of reservoir solution consisting of 0.2 M Sodium sulfate (Molecular Dimensions ...
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bioRxiv - Plant Biology 2022Quote: Total protein extracts were prepared by re-suspending 100 mg of ground plant material in 100 μl of protein lysis buffer (50 mM Tris pH 8.0; 2% SDS; 10 mM EDTA; protease inhibitors (Roche (04693159001) cOmplete™ ...
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bioRxiv - Neuroscience 2022Quote: One adult rat brain (2 g) was homogenized in nine volumes of 320 mM sucrose solution supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and Phosphatase Inhibitor Cocktail 1 and 2 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Immunology 2023Quote: ... Tumors excised at day indicated in each figure were disaggregated mechanically and enzymatically with 2 mg/mL collagenase IV and 50 U/mL DNase I (Roche).
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bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
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bioRxiv - Neuroscience 2023Quote: Mouse brains at 5W were isolated and homogenized in homogenization buffer [(0.32 M sucrose, 10 mM HEPES, 2 mM EDTA and 1× complete protease inhibitor cocktail (Roche Diagnostics), pH 7.4)] ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in 4x pellet volume of sucrose buffer (10 mM HEPES/KOH pH 7.5.; 2 mM MgAc; 250 mM Sucrose, 1x Protease inhibitor cocktail [Roche, Germany]) and lysed with ~50 strokes in a tight-fit dounce homogenizer ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
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bioRxiv - Genomics 2022Quote: ... Re-purified circular fragments were amplified by PCR with View-Point specific primers (see Supplementary Table 2) using the Expand Long Template PCR System (Roche). Resulting amplicons were purified with a 0.8× ratio of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... the cell pellets resuspended in lysis buffer (purification buffer 50 mM Tris pH 8, 50 mM NaCl, 2 mM DTT; + cOmplete EDTA-free Protease Inhibitor (Roche)) and lysed by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated for 1 hour in TBST and then in Blocking Buffer (2% blocking reagent in maleic acid buffer pH 7.5 (Roche, #11096176001) and 10% sheep serum (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The suspension medium was carefully removed and replaced with fresh PDB supplemented with 2× protease inhibitor (Mini Protease Inhibitor Cocktail, Roche), 0.5 mM or 2 mM PMSF (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... The agarose was suspended in dilution buffer for IPs from cell lysates and in a 2 : 1 lysis buffer : dilution buffer mix for IPs from EC fractions and supplemented with cOmplete Protease Inhibitor Cocktail (Roche). Of the 130 µl EC fraction/cell lysate ...
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bioRxiv - Genomics 2024Quote: ... were digested for 1 hour at 37°C under 110 rpm shaking conditions in DMEM supplemented with 2 mg/mL collagenase D (Roche) and 0.4 mg/mL Dispase (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 times with PBS (2 minutes per wash) followed by the addition of secondary antibodies prepared in the blocking buffer along with DAPI (Roche, 10236276001 ...
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bioRxiv - Molecular Biology 2024Quote: ... the nuclei were resuspended in 1.2 ml of NEB3 buffer (10 mM Tris-HCl [pH 8.0], 1.7 M sucrose, 2 mM MgCl2, 0.15% Triton X-100, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... 100 nM of calyculin A, 2 mM of DTT, 100 U/mL of RNasin, 100 mg/mL of cycloheximide, and Roche protease and phosphatase inhibitor cocktails ...
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bioRxiv - Microbiology 2024Quote: ... and M2 polarized phagocytes 2 wells per condition were activated overnight with LPS (0.5 µg / mL) and IFN-γ (10 ng / mL, Roche) for M1 or 20 ng / mL IL-4 for M2 at 37 °C with 9.5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 U/ml Pen-Strep, 10 mM HEPES, and 2% FBS freshly supplemented with 100 μg/mL of Liberase TM [Roche, #5401127001] and 100 μg/mL of DNase I [Zymo Research ...
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bioRxiv - Genomics 2024Quote: mESCs containing an RMCE cassette (detailed in a prior study by Lienert et al.2) were subjected to hygromycin selection (250 μg/ml, Roche) for a period of 10 days ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 2 hours at room temperature in 1% blocking solution (1% Western Blocking Reagent (WESTBL-RO, Roche) in TBS ...
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bioRxiv - Immunology 2024Quote: ... Libraries were amplified over 12 cycles in thermocycler using KAPA HiFi HotStart ReadyMix and 5µM Pre-LM-PCR Oligo 1&2 (Roche, Switzerland). Following amplification another 3-step adapter removal with AMPure XP beads was carried out ...
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bioRxiv - Cell Biology 2024Quote: ... then with 1 ml of Western blot buffer (2% SDS, 5% Glycerol, 50 mM Tris-HCl, 0.2 M EDTA + cOmplete protease inhibitor cocktail tablet (Roche, 11697498001) + PhosSTOP (Roche ...
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bioRxiv - Systems Biology 2024Quote: The eluted samples were used as a template for a PCR reaction for amplifying Tn5 tagmented fragments and sample barcoding (2× Kappa HIFI [Roche] ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies used were prepared in 2% BSA (diluted in PBS) and used at the following concentrations: rat monoclonal anti-HA (1:1000, 3F10 Roche), mouse monoclonal anti-myc (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... 5 mM of MgCl2, 2 mM of DTT, 1% NP40, 100 U/mL of RNasin, 100 mg/mL of cycloheximide, and Roche protease and phosphatase inhibitor cocktails) ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed using gene-specific primers (listed in Supplementary Table 2) in a total volume of 10 µL SYBR Green Master mix (Roche), on a Lightcycler LC480 apparatus (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... 750 mM KCl) supplemented with 40 U of RNase inhibitor (Beyotime) and 2 μL of biotin RNA labelling mix (Roche) and incubated at 37°C for 15 minutes for the purpose of elongation and biotinylated UTP integration ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of plasmids encoding GFP-tagged proteins were transfected using 2 μL X-tremeGENE HP DNA Transfection Reagent (Roche) per well ...
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bioRxiv - Genomics 2024Quote: ... primary antibodies diluted in Wash+ buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 0.05% triton-X100, 2 mM EDTA, 1% BSA, with Roche cOmplete protease inhibitor) overnight at 4° ...
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bioRxiv - Genomics 2024Quote: ... supplemented with protease/phosphatase inhibitors (1 mM PMSF, 2 mM Na3VO4, 20 mM Na4P2O7, 50 mM NaF, complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... master mix and qPCR primers for one-step relative quantification of target genes and 2 reference genes using a 384-well format LightCycler 480 qPCR system (Roche) based on our previously published protocols (89–92 ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 300 mM NaCl, 1 mM DTT, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Developmental Biology 2024Quote: 14 hpf embryos generated by intercrossing wild-type ABs were dechorionated in petri dishes coated with 0.5% agarose using E2 media (Westerfield, 2007) containing 2 mg/ml Pronase (Roche, 10165921001) at 25°C for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were incubated for 1–2 days depending on colorization with a mixture of 250 μg/mL NBT (#11 585 029 001; Roche) and 130 μg/mL BCIP (#11 383 221 001 ...
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bioRxiv - Immunology 2020Quote: ... and a 30-gauge needle was used to inflate each pancreas through the common bile duct with 4 mL of media supplemented with 0.8 mg/mL of collagenase P (Roche, cat. number 11-249-002-001) and filtered at 0.22 μm prior to injection ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μM dNTPs (with 3:1 mix of 7-deaza-dGTP:dGTP; dNTPs from New England Bio Labs, cat#N0446, 7-deaza-2’-deoxy-GTP from Roche, cat#10988537001), 0.8 M Betaine (Alfa Aesar ...
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: ... Size selection using SPRI-beads isolated low molecular weight DNA which was then PCR amplified using 2 × HiFi HotStart Ready mix (Roche Diagnostics) and Nextera Indexing Primers (Illumina ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...