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Citations for Roche :
2001 - 2050 of 3223 citations for Mouse Anti Hepatitis C Virus E1 Antibody 1879 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... or HA-HRP antibody (12-013-819; 1:3,500; Roche). The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL ...
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bioRxiv - Developmental Biology 2021Quote: ... Excess antibody was removed with dig wash buffer (Roche, 11585762001) before staining with NBT and BCIP in 100mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... DIG was detected with an alkaline phosphatase labeled antibody (Roche).
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bioRxiv - Cell Biology 2020Quote: GFP-fusion proteins were detected using a monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... Bound antibodies were revealed using Chemiluminescence Blotting Substrate from Roche according to the manufacturers’ suggestions.
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bioRxiv - Biochemistry 2022Quote: ... The horseradish-peroxidase coupled HA antibody was ordered from Roche (Anti-HA-Peroxidase ...
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bioRxiv - Cancer Biology 2023Quote: ... and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790-4286). Images were captured with VENTANA iScan HT (Roche Diagnostics ...
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bioRxiv - Biochemistry 2023Quote: ... The horseradish-peroxidase coupled HA antibody was ordered from Roche (Anti-HA-Peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were analyzed by immunoblotting using antibodies against GFP (Roche Applied Science ...
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bioRxiv - Neuroscience 2023Quote: ... or incubation with OmniMap Rabbit HRP antibody (760-4311, Roche) and ChromoMap DAB (760-159 ...
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bioRxiv - Cancer Biology 2023Quote: Secondary antibodies were conjugated with horseradish peroxidase (OmniRabbit, Ventana, Roche) and the immunohistochemical reaction was developed using 3,30-diaminobenzidine tetrahydrochloride (DAB ...
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bioRxiv - Microbiology 2023Quote: ... The correct clones were further confirmed using HA antibodies (Roche) in both immunofluorescence imaging and western blotting.
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bioRxiv - Neuroscience 2024Quote: Primary antibodies used for Western blotting include GFP (11814460001; Roche), pThr73-Rab10 (ab230261 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by sequential staining with primary antibody for CD31 (Roche) and PLVAP (Proteintech) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used: rat α-HA (3F10, Roche Applied Sciences), mouse α-Centrin1 (clone 20H5 ...
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bioRxiv - Plant Biology 2019Quote: ... 4 °C) the cleared supernatants were diluted 1 time using water containing 1x cOmplete ™ EDTA-free protease inhibitor (Roche). Protein extract was incubated overnight with anti-HA antibodies (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... Potential residual cellular phosphatase activity was blocked by incubating HRas samples for 10 min at 37 °C with PhosSTOP (Roche). HRas samples were subsequently incubated for 20 min at 37 °C with 10 mM EDTA and a 15X molar excess of either GMPPNP or GDP ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell suspensions incubated for 10 min at 4°C in SDS lysis buffer containing protease inhibitors (Complete protease inhibitor cocktail, Roche). The remaining procedures were performed as described in previous reports (Carvajal-Gonzalez et al. ...
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was equilibrated in hybridisation buffer for 1 hour at 68°C in pre-warmed DIG Easy Hyb solution (Roche) in a rotating hybridisation oven ...
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bioRxiv - Molecular Biology 2021Quote: ... exponentially growing cells were treated with DMSO or 1 μM PARPi for 24 h followed by 45 min incubation at 37°C with Colcemid (Roche). Metaphase preparation and aberration analysis were performed as it follows ...
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bioRxiv - Developmental Biology 2020Quote: To prepare nuclear extracts all procedures were done at 4°C and buffers supplemented with 1x protease inhibitors (Roche, 4693132001), 15 μM MG-132 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and age-matched controls lacking evident tau pathology were gently homogenized at 4°C in 10 mL of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... primary antibodies were incubated at 37°C for 1hr and Discovery multimer detection system (Discovery OmniMap HRP, Discovery DAB and Purple, Roche) was used to detect and amplify immuno-signals ...
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bioRxiv - Genomics 2019Quote: ... An additional Proteinase K incubation (65 μl of 10mg/mL per 7 million cells starting material) at 65°C for two hours was followed by RNase A (Roche; 15 μl of 10mg/mL per 7 million cells starting material ...
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bioRxiv - Bioengineering 2019Quote: ... For molecular cloning 3 µg of RNA were reverse transcribed for 30 min at 50°C using an oligo(dT) primer and the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) followed by 5 min enzyme inactivation at 85°C according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... 25ml of pre-warmed to 37°C EGTA buffer followed by 25ml of pre-warmed to 37°C EBS buffer with 2.3U of Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) were cannulated into the vena cava ...
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bioRxiv - Cell Biology 2019Quote: To generate a peptide spanning lysin 105 and 106 the immunoprecipitates were digested in solution with 400 ng Glu-C (Sequencing Grade, Roche) at 37C for 16 hours.
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bioRxiv - Genomics 2019Quote: ... To remove RNA in the RNA:cDNA duplex the sample was incubated for one hour at 37° C with 125 mU/µl RNase H (Y9220F, Enzymatics) and 500 ng/µl RNase A (11579681001, Roche) in 1 × Rnase H buffer (Y9220F ...
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bioRxiv - Developmental Biology 2019Quote: ... probes were synthesized at 37°C for 2-4 hrs in digoxigenin-synthesis reaction mixture with T3 RNA polymerase (Roche). After synthesis ...
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bioRxiv - Neuroscience 2019Quote: ... The suspended pellet was incubated under agitation for 30 min at 37 °C and then digested at 56°C for 30 min with 20 μl of 50 mg/ml proteinase K (315836; Roche) in 200 μl of buffer AL ...
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bioRxiv - Molecular Biology 2019Quote: ... Following the preparation of plates an incubation at 4°C for at least 60 min was performed before the plates were analyzed on a LightCycler 480 II (Roche) using 45 cycles and a temperature profile recommended by the manufacturer for miRCURY SYBR® Green mastermix ...
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bioRxiv - Microbiology 2019Quote: ... The reaction was conducted in a Versant kPCR (Siemens) at 65°C for 40 minutes for BAL samples and in a Lightcycler (Roche) at 65ºC for 30 minutes for EA/BAS ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Immunology 2021Quote: ... The reverse transcriptase was inactivated at 70 °C for 15 min and single stranded cDNA was subsequently amplified using Kapa HiFi HotStart Readymix (Roche) at a 1x concentration together with 250 nM UP-primer* (Metabion ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
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bioRxiv - Bioengineering 2020Quote: ... Tissues were finely diced manually with standard razor blades and digested for 45 min at 37°C with 1.67 Wünsch U/mL (0.5 mg/mL) Liberase TL (Roche Diagnostics, Sigma Aldrich) and 0.2 mg/mL DNase I (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... the blot was washed at 55°C and probe detection was performed according to the manufacturers’ protocol using the DIG luminescent detection kit (Roche). The intensity of individual bands was measured with respect to the background and integrated density value (IDV ...
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bioRxiv - Plant Biology 2021Quote: ... Northern blot analysis was performed at 50°C using protocol provided in the DIG Application Manual for Filter Hybridization (Roche). Following the hybridization ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... The eluted chromatin was then treated with RNaseA (10mg/mL) for 1 hour at 37°C and with Proteinase K (Roche) for 2 hours at 55°C ...
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bioRxiv - Cell Biology 2022Quote: mRNA was purified from ∼200 ng of total RNA with oligo-dT beads and sheared by incubation at 94°C in the presence of Mg2+ with the KAPA mRNA HyperPrep kit (Roche). Following first-strand synthesis with random primers ...
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bioRxiv - Microbiology 2022Quote: PPs were excised from the SI and digested (37 °C, 45 min) using 100 µg/ml liberase TH/DNase (Roche) in RPMI containing 5 % FCS ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche] ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... This was spun down (5 minutes at 1800 RMP, 4°C) and the pellet then digested in 10ml collagenase/dispase (1mg/ml; Roche) and DNase I type IV (40µg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was immobilized using UV crosslinking (125 mJ/cm2) and hybridized at 42°C using Abion ULTRAhyb hybridization buffer and PCR DIG probes (Roche) synthesised according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...