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Citations for Roche :
2001 - 2050 of 7151 citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... cells were harvested through centrifugation at 5000 rpm for 10 min at 4°C and afterwards washed with 2mL PBS supplemented with 1x protease inhibitors cocktail (Roche, Basel, Switzerland). Cell pellet was resuspended in lysis buffer (150 mM KCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 40 mM Tris-HCl pH 7.5, 20 mM MgCl2, and 4% Triton X-100 supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail), and 24 ml nuclease free water on ice for 20 min with frequent mixing ...
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bioRxiv - Microbiology 2021Quote: ... 500 mM NaCl and 4 mM β-mercaptoethanol (β-ME)) in the presence of an anti-protease cocktail (Complete EDTA free, Roche) and 1 ul benzonase (MERK Millipore 70746) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 10,000 RPM for 30 minutes at 4°C and resuspended in 40mL lysis buffer (20mM HEPES, pH 7.4, 1M NaCl, 1mM EDTA, 1mM DTT, Roche protease inhibitor cocktail). Lysozyme was added at a final concentration of 1mg/ml and cells were incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... To determine the dimerization of sFeoFL::GFP and sFeoN:GFP a Western Blot was generated from a 4-12% gradient SDS and a Native polyacrylamide gel side-by-side using a mouse anti-GFP antibody (Roche Nr. 11814460001) at 1:500 dilution ...
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bioRxiv - Genomics 2022Quote: ... then incubated with primary antibody at 4°C overnight in Wash Buffer (10 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) with 2mM EDTA ...
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bioRxiv - Microbiology 2022Quote: ... 0.2 μL of each primer (100mM stock) (Table SI-4) and 5 μL of 2× KAPA SYBR Fast Universal qPCR kit (KAPA Biosystems, USA). Samples were cycled (40 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... was first pooled before being divided into 700-µL aliquots in cryovials containing 28 µL of a 25x concentrated protease inhibitor cocktail solution (4% v/v) (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) according to the manufacturers’ instructions and stored at -80 °C (Figure 10 – step III).
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bioRxiv - Neuroscience 2022Quote: ... The cell viability following 4-AP treatment was assayed by MTT assay according to manufacturer’s protocols (Roche, cell proliferation kit I (MTT), # 11465007001) ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
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bioRxiv - Pathology 2020Quote: ... The specific pairs of primers used for the real-time PCR are listed in Supplementary Table 4 (designed by Universal ProbeLibrary Assay Design Center tool from Roche, Indianapolis, IN). The real-time PCR was performed with FastStart Universal Probe Master mix ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed in 4 replicates in final volume of 10 µL using Light SYBR Green PCR Master Mix (Roche, Applied Science) on a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell pellets were lysed in 300 µL of lysis buffer (10 mL BugBuster® [Millipore 70584-4], ¼ tablet cOmplete™ Protease Inhibitor Cocktail EDTA-Free [Roche]) for 20 min at room temperature and centrifuged at 13,000 rpm at 4℃ for 30 min to clear all cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 500 x g for 5 min at 4°C and resuspended in 10 ml 4 °C RSB supplemented with protease inhibitor (cOmpletetm, EDTA-free protease inhibitor cocktail, Roche, Cat#: 11873580001) and incubated on ice for 15min ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Immunology 2021Quote: ... 20 mM Tris pH 7.5, 1% Triton X-100 supplemented with 1× protease inhibitor and 1× phosphatase inhibitor; Roche, Basel, Switzerland). Lysates were cleared by centrifugation at 10,000 r.p.m ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared for 1 hr using 30 μl dry volume of a 1:1 mixture of protein-A and protein-G agarose (Roche Diagnostics). Solubilized membranes (3 mg of total membrane protein ...
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bioRxiv - Biochemistry 2019Quote: ... 1 % Tween-20 and 1 mM β-mercaptoethanol supplemented with 1 mg/mL lysozyme and protease inhibitor cocktail tablet (Roche, Switzerland) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Tween 20) for 20 min followed by 1 h incubation with alkaline phosphatase-anti-digoxigenin antibody (1:500, Roche, 1093274). Alkaline phosphatase activity was detected by incubating slides with Fast Red substrate (DAKO ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 pseudotyped virus was generated by co-transfection of the pCAGGS-S with the viral packaging plasmid psPAX2 and pLenti-EGFP/luciferase-expressing plasmid as a proportion of 1:1:1 into HEK293T cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001) according to the manufacturer′s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were subsequently incubated in 1 ml of blocking solution (1:1 (v/v) of Western Blocking Reagent (Roche, Cat#11921681001) and PBT ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, 1x Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 0.5 mM PMSF, 1 mM DTT and 1×Complete inhibitor tabs, Roche, for 15 min), then treated with 10% NP40 and vortexed ...
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bioRxiv - Developmental Biology 2022Quote: ... 5x SSC, 0.05mg/ml Heparin, 0.25% Tween20, 1% SDS, 1 mg/ml Salmon Sperm DNA, 1 mg/ml Roche blocking buffer powder) containing oligo probes at a concentration of 1μg/120μl and hybridized for two days at 43°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated with the secondary antibodies 1:1000 in PBST supplemented with 1 % DMSO and 1 % blocking reagent (Roche, #1096176) overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... 0.2 mM EDTA, 0.3 M NaCl, 1 M Urea, 1% IGEPAL CA-630, 1 mM PMSF, and Roche protease inhibitor cocktail) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... If stained with phalloidin (1/500, Scientific Laboratory Supplies P1951) and DAPI (1/1000 in PBS from 1 mg/ml stocks, Roche 10236276001), or just DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... 1 X protease inhibitor cocktail and 1 X phosphatase inhibitor cocktail from Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... reaction buffer containing 1 μL lambda phosphatase plus 1×phosphatase inhibitors cocktail (Roche) in at 30°C for 30 min with gently shaking ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mM EGTA and 1 mM DTT) supplemented with cOmplete protease inhibitors (Roche), benzonase (Merck ...
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bioRxiv - Immunology 2021Quote: ... and 10% glycerol) containing 1 mM PMSF and 1 × protease inhibitor cocktail (Roche). Then ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mM EGTA and 1 mM DTT) supplemented with complete protease inhibitors (Roche), benzonase (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EGTA and 1% Triton X-100 with protease/phosphatase inhibitor (Roche) mixture ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) with 1 mM PMSF and protease inhibitor cocktail tablets (Roche) by vigorous shaking in a fast prep cell breaker (Bio 101 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM DTT and 1× cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Fifty micrograms of total protein were loaded onto SDS-PAGE gels and separated by electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM EDTA and supplemented with 1× Complete protease inhibitor cocktail (Roche) by using a Teflon pestle (Schuett Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1 mM EDTA and 1×Complete EDTA free-tablet (Roche Diagnostics, Basel, Switzerland) in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... in a 1:1 ratio and added to white 384 well plates (Roche). Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).