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Citations for Roche :
151 - 200 of 438 citations for palmitic acid ethoxylated phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested and resuspended in twice the cell pellet volume of lysis buffer (50 mM potassium phosphate pH 8.0, 0.5 M NaCl, 20 mM Imidazole, cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Genetics 2022Quote: ... 20-30mg of liver was homogenised in 400μl of cold homogenising buffer (50mM phosphate buffer pH 7.5 and 1x Roche EDTA-free protease inhibitor (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... In vitro translation reactions with luciferase reporter mRNAs were carried out with 0.5x extract, energy mix (final 0.84 mM ATP, 0.21 mM GTP, 21 mM creatine phosphate (Roche 10621722001), 45 U/mL creatine phosphokinase (Roche 10127566001) ...
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Transmembrane domains of H1 and H3 hemagglutinin contribute to membrane fusion in a different mannerbioRxiv - Biophysics 2024Quote: Plant leaf tissue was weighed and homogenized in the isolation buffer (0.1 M sodium phosphate, pH 7.2) supplemented with a protease inhibitor cocktail (cOmplete, Roche) using a Waring blender ...
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bioRxiv - Developmental Biology 2020Quote: ... The embryos were blocked in Blocking Buffer + Maleic Acid (Roche, 11585762001) for at least 3 hours before the anti-DIG antibody was added in a concentration of 1:5000 and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5% deoxycholic acid) containing inhibitors of phosphatases (1:10 PhosphoStop; Roche) and proteases (1:100 Protease Inhibitor Cocktail ...
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bioRxiv - Biochemistry 2020Quote: ... followed by optional sialic acid removal using 0.02 U sialidase (Roche), prior to in-gel trypsin treatment49 ...
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bioRxiv - Plant Biology 2020Quote: ... Digoxigenin was detected using DIG Nucleic Acid Detection Kit (Roche, USA).
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bioRxiv - Systems Biology 2021Quote: ... 2% fatty acid-free bovine serum albumin (BSA) fraction V (Roche), 50 μmol/L 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 0,002% mellitic acid and 1 pastil of protease inhibitors cocktail (Roche)).
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bioRxiv - Neuroscience 2021Quote: ... 25 nM okadaic acid and a protease inhibitor cocktail tablet (Roche). Soluble and insoluble fractions of total homogenates were obtained as previously described (30) ...
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bioRxiv - Neuroscience 2023Quote: ... and ethylenediaminetetraacetic acid-free Complete Protease Inhibitor Cocktail (Roche Applied Science). After centrifugation at 20,000[×[g for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 1 tablet crushed complete EDTA (ethylenediaminetetraacetic acid)-free protease inhibitor (Roche), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Biophysics 2022Quote: ... 1 tablet ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche diagnostics, GmbH), pH 8) ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection was accomplished using the DIG Nucleic Acid Detection Kit (Roche). Images were quantified using ImageJ ...
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bioRxiv - Molecular Biology 2020Quote: ... washed six times with phosphate buffered saline (PBS) and resuspended in 0.6ml of PBS supplemented with protease inhibitory cocktail (Roche) as per manufacturer’s specification ...
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bioRxiv - Biophysics 2020Quote: ... pellet was resuspended in lysis buffer (10 mM phosphate buffer saline pH 7.3, supplemented with cOmplete protease inihibitor, EDTA free (Roche) and 1 mM Phenylmethylsulfonyl fluoride (Sigma)) ...
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bioRxiv - Biochemistry 2020Quote: Cell pellets were immediately resuspended in buffer A (50 mM sodium phosphate, pH 7.4, 300 mM sodium chloride) and supplemented with a protease inhibitor tablet (Roche), 100 μM PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... NPCs were washed by phosphate buffer saline (PBS) and stained by in situ Cell Death Detection Kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 × g 20 min) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were lysed (Avestin Emulsiflex C5) in lysis/binding buffer (20mM phosphate [pH7.4], 500mM NaCl, 20mM imidazole, protease inhibitor cocktail [Roche]), clarified lysate was applied to a 5mL HisTrapHP column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in MABTr and then incubated in NBT (nitro blue tetrazolium)/BCIP (bromo-chloro-indolyl-phosphate, Roche) for colour development ...
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bioRxiv - Biochemistry 2022Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 x g 20 min) ...
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bioRxiv - Biophysics 2021Quote: ... Cells were resuspended in 100 ml of lysis buffer (20 mM sodium phosphate pH 7.4, 500 mM NaCl with DNase I (Roche), 0.5 mM MgCl2 and 1 mM PMSF) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bronchoalveolar lavage (BAL) was collected by instilling the lungs with phosphate-buffered saline containing cOmplete protease inhibitor cocktail (Roche) (0.5 mL x 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were re-suspended in lysis buffer (phosphate buffered saline containing 0.5% Triton-X 100 and complete protease inhibitors [Roche]) and DAPI was added (final concentration 5μM ...
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bioRxiv - Plant Biology 2021Quote: ... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche).
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bioRxiv - Molecular Biology 2021Quote: ... After removing the supernatant, spheroplasts were resuspended in Ficoll buffer (18% FICOLL, 20 mM phosphate buffer pH 6.8 (PI-Roche, 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% heat-inactivated lamb serum in Tris-buffered saline with 0.1% Tween-20 (TBST) and incubated with alkaline phosphate-conjugated anti-DIG antibody (1:2000, Roche) in blocking buffer overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Systems Biology 2023Quote: Glucose concentrations from the chemostat samples were determined enzymatically with a solution of hexokinase/glucose-6-phosphate dehydrogenase (Roche) in Pipes buffer at pH 7 ...
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bioRxiv - Microbiology 2023Quote: ... washed one time with 1 ml phosphate-buffered saline (PBS) and resuspended in 0.3 ml PBS supplemented with complete mini protease Inhibitor Cocktail (Roche). Cell suspensions were transferred to lysis tubes containing glass beads and subjected to mechanical disruption in a homogenizer SpeedMill Plus (Bioanalytik Jena ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were subsequently washed with phosphate-buffered saline (PBS) and re-suspended in PBS containing protease inhibitors (Roche, Canada). Cells were heated at 54°C for 3 min in a thermal cycler (VeritiPro ...
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bioRxiv - Molecular Biology 2023Quote: ... The Ni-NTA agarose was rotated for 30 min at 4°C with 10 mL of elution buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 1.4 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Biochemistry 2023Quote: ... The cell pellet was resuspended in 300 µL of buffer containing 10 mM Na-phosphate buffer (pH 7.0) and EDTA-free Protease inhibitor (Roche). Soluble and membrane fractions were isolated by lysing cells with three freeze-thaw cycles (−20°C to room temperature) ...
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bioRxiv - Genetics 2023Quote: VSMCs and myofibroblasts were scraped in phosphate-buffered saline (PBS) supplemented with protease inhibitor cocktail (1:100, Roche, 11836145001) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Cell Biology 2022Quote: Kidney cortices were homogenized in a buffer containing 50 mM pH 7.4 potassium phosphate and cOmplete Protease Inhibitor Cocktail tablet (Roche, Indianapolis ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were ruptured using a beadbeater with 1.9g of glass beads either in SDS buffer (1X phosphate buffered saline, 10% Glycerol, 2mM EDTA, 2mM DTT, Roche protease inhibitor cocktail ...
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bioRxiv - Genetics 2022Quote: ... 20-30mg of liver was homogenised in 400μl of cold homogenising buffer (50mM phosphate buffer pH 7.5 and 1x Roche EDTA-free protease inhibitor (Roche, Switzerland)) using Precellys homogeniser tube (VWR ...
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bioRxiv - Biochemistry 2022Quote: The cell pellet was resuspended in Buffer A (50 mM sodium phosphate, 150 mM NaCl, pH 7.4) and supplemented with a protease inhibitor (Roche), 100 μM PMSF ...
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bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Plant Biology 2023Quote: ... the cells were harvested in 20 mM potassium phosphate buffer (pH 8.0) containing 500 mM NaCl and cOmplete EDTA-free protease inhibitor cocktail (Roche). The inclusion bodies were pelleted from sonicated cells at 3000 × g for 15 minutes and solubilized in 20 mM potassium phosphate buffer (pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM pyridoxal 5’-phosphate hydrate (PLP) and proteases inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail Roche).
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bioRxiv - Biochemistry 2024Quote: His-tagged 53BP1 TTD was purified by resuspending thawed cell pellets in 30ml of lysis buffer (50mM sodium phosphate pH 7.2, 50mM NaCl, 30mM imidazole, 1 x EDTA free protease inhibitor cocktail (Roche) per litre of culture) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were later defrosted and resuspended in 20 mM Potassium Phosphate buffer pH 7.6 with 300 mM NaCl and a protease inhibitor cocktail (Roche) together with DNase I (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... the pellet was resuspended in 45 µl of sodium phosphate buffer supplemented with EDTA-free protease inhibitor cocktail (Roche) and incubated at 37°C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Each brain was gently homogenized in 950 µl phosphate-buffered saline (PBS, pH ∼7.4) containing Complete EDTA-free Protease Inhibitor Cocktail (Roche). The homogenization was done with 25 strokes in 2 ml homogenizer ...
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bioRxiv - Molecular Biology 2020Quote: ... in Tris calcium buffer with ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche) were added and the pellet resuspended ...