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Citations for Roche :
151 - 200 of 268 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Tissue pieces were then moved to a 6-well plate and incubated with Collagenase D (0.24U/mg, Roche) and DNase I (10U/µl ...
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bioRxiv - Immunology 2024Quote: ... into 96-well PCR plates with 1 μL of cell lysis solution (1:10 Cell Lysis buffer [Roche] ...
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bioRxiv - Genetics 2024Quote: ... kit was used in all qPCR eDNA runs on 96-well reaction plates (Roche Diagnostics GmbH, Mannheim, Germany) with the parameters shown in Fig 3 ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR (qPCR) using a 96-well plate in a pre-heated LightCycler 480 Instrument II (Roche, Basel, Switzerland) was used to quantify the copy number of the 16S rRNA genes in the samples as previously described 117 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-μl aliquots of all cDNA samples were analyzed in triplicate on 96-well optical PCR plates (Roche Diagnostics). GAPDH or TBP was used as the reference gene and all analyses were performed using the ΔΔCt method with Roche LightCycler 96 system software ...
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bioRxiv - Developmental Biology 2020Quote: ... Neural stem / progenitor cells were seeded in coated 24-well plates (1.6×105 cells per well) and transfected with the reporter plasmids using xTremeGENE HP DNA transfection reagent (Roche). Cells were collected 48hr after transfection using Passive lysis buffer (Promega) ...
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bioRxiv - Microbiology 2022Quote: Aag2 or U4.4 cells were plated in 48-well plates and transfected with 100 ng of dsRNA per well using 0.4 µL of X-tremeGENE HD (Roche). The medium was refreshed after 3 h and cells were incubated for 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were plated onto collagen-coated plates and transfected at 60-80% confluency using FuGENE 6 (Roche, Indianapolis, IN) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Systems Biology 2021Quote: ... cell culture counts were accessed in the count plate on an automated cell counter (Cedex, by Roche, Basel, Switzerland). As there was little or no growth lag after splitting the culture ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected into COS-7 cells (14,000-17,000 cells/cm2 in 12-well plate) for 24 h using X-tremeGENE HP DNA (Roche, #06366236001). Cells were incubated for 7 min at 37 °C with either 4 μg.mL-1 rabbit anti-γ2 Ex2 or 1:50 serum rabbit anti-γ8 Ex1 antibodies prior to fixation ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed eight times with PBST followed by the addition of 50 μL ABTS solution (Roche, #11684302001) into each well ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed in optical 96-well plates using SYBR® green in a Light Cycler 480 (Roche). Primers were designed using Primer-Blast (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed in optical 96-well plates using SYBR® green in a Light Cycler 480 (Roche). Primers were designed using Primer-Blast (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) ...
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bioRxiv - Molecular Biology 2021Quote: ... The experiments were carried out in 96-well reaction plates with a Light Cycler 96 Instrument (Roche, Basel, Switzerland). The reaction conditions were as follows ...
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bioRxiv - Physiology 2022Quote: ... In each reaction 50 ng luciferase reporter and 150 ng expression vectors were transfected into C2C12 myoblasts in 24-well plates (50,000 cells per well) using X-tremeGENE 9 (Roche). 5 ng modified pRL null plasmid (25 ...
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bioRxiv - Microbiology 2022Quote: ... a 12.5 μl reaction/well was prepared in 4titude® 96-Well Semi-Skirted PCR Plates for LC480 (Roche) using a SuperScript III One-Step RT-PCR Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: qRT-PCR was performed in triplicates in 384-well plates using primers and Roche universal probe library (Roche, 04869877001) with TaqManTM Universal MasterMix II (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... 9 μl of qPCR reaction was set up in 384 well plates with 4.5 μl of 2X FastStart Universal SYBR Green Master mix ROX (Roche), ∼0.28 μM of primers (Set 1 ...
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bioRxiv - Genomics 2023Quote: ... Cells were washed twice in cold PBS and scraped off the plates in cold PBS containing protease inhibitors (Roche, Complete 11836170001 and Halt ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in 384-well plates with FastStart Universal SYBR Green Master Mix (04913914001, Roche) on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed in 96-well plates on a Roche LC 480 or z480 light cycler (Roche, Basel, Switzerland) according to the CDC procedure including a previously negative patient specimen (extraction and RNaseP internal controls) ...
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bioRxiv - Genomics 2020Quote: ... Entire material of one conversion was equally distributed to a 96-well plate and amplified via PCR using KAPA HiFi Uracil+ Kit (Roche) in a total volume of 16µl ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
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bioRxiv - Biophysics 2020Quote: HeLa cells were plated in 6-well plates and infected with virus at equal amounts of p24 after treatment with DNaseI (Roche) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells in culture were seeded into 96 well plates and transfected in suspension using X-tremeGENE HP DNA Transfection Reagent (Roche). Transfection complexes were prepared such that each reaction contained 11 μL of Opti-MEM ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed using a 96-well plate in a pre-heated LightCycler® 480 Instrument II (Roche, Basel, Switzerland). Each well contained a 10 μl reaction mixture comprising 1 μl DNA template ...
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bioRxiv - Cancer Biology 2020Quote: ... CAL33-c5 and CAL33-c11were seeded in 96-well plates and cell viability was determined with the colorimetric Cell Proliferation Kit II (XTT) (Roche) 24 ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were spun (420 RCF x 2 min on LCM-3000 plate centrifuge (Grant Instruments, Royston, UK) before analysis on the Light Cycler 480 (Roche). Samples were run for 50 cycles (10s at 95°C and 30s at 60°C) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
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bioRxiv - Biophysics 2022Quote: ... For a given RT-qPCR reaction 10 nM of RNA were incubated in water with DMSO or 10 μM of small molecules at room temperature for 20 mins in a 96 well lightcycler plate (Roche). During incubation an RT-qPCR mastermix was prepared by adding 25 μL of 1 step SYBR mastermix (QuantaBio) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... resuspended at 1 million cells per mL in CETSA buffer and dispensed (10 μL per well) into 384-well PCR plates (Roche) using a Multidrop Combi (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 and various concentrations of ADP-ribose or hit compounds were mixed on ice in 384-well PCR plate (Roche). Fluorescent signals were measured from 25 to 95 °C in 0.2 °C/30/Sec steps (excitation ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were plated in 6-well plates (100,000 cells/well) and transfected the next day using X-tremeGENE™ 9 DNA (Transfection Reagent, Roche), with HA-NLGN1 + AP-MDGA1 or AP-MDGA2 + BirAER (1 μg/well) ...
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bioRxiv - Biochemistry 2020Quote: ... was added to a final concentration of 5X and mixtures were aliquoted to a final volume of 15 µL into a LightCycle 96-well white qPCR plate (Roche). Thermal denaturation was conducted in a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2022Quote: ... The plate was sealed and centrifuged (2 minutes, 180×g, 4°C) before analysis with the LightCycler 480 Instrument II (Roche; Preincubation ...
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bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Biochemistry 2021Quote: Cells confluent in a 15-cm dish were washed by PBS buffer three times before being lysed on plate with 1 mL ice-cold NP-40 lysis buffer supplemented with protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA expression was quantified in technical triplicates using RT-qPCR performed in 384-well plates on a Roche LightCycler 480 Instrument II with dual hybridisation probes from The Universal ProbeLibrary (Roche). Oligonucleotide sequences used for RT-qPCR are listed in Appendix Table S3 ...
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bioRxiv - Bioengineering 2020Quote: ... Hela cells were plated one day before in 6-well plate at 50% confluency and transfected with 1 μg plasmid and 3 μl XtremeGeneHP transfection reagent (Roche) during 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... and low-volume supernatants (90 μL media per well of a 48-well plate) were mixed 1:1 with 2× SDS/PAGE sample buffer containing Complete Mini EDTA-free Protease Inhibitor Mixture (Roche). In experiments where primary hMDMs were plated in a 24-well plate and infected with T4SS-Lp ...
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bioRxiv - Genomics 2021Quote: ... Early passage cells were seeded at 25% confluency in six-well plates and transfected with 2 ug of expression vector using Fugene reagent (Roche). Cells were harvested and seeded into 100 mm dishes after 48 h of transfection ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using SYBR green technology in 96 well optical plates with lightcycler 480 real-time PCR machine (Roche) adopting the following thermocycle conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time reverse transcription PCR assays were conducted in 96-well plates using the LightCycler 480 Instrument (Roche, Wilmington, MA). Levels of mRNA expression were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Cell Biology 2022Quote: ... was used to analyze 4.5 µl of the cDNA dilution in 10 µl reactions on a white LightCycler® 480 Multiwell Plate 384 (Roche). To confirm the absence of PCR contamination ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
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bioRxiv - Genomics 2023Quote: ... The batches of isolated RNA were arranged sequentially in 96-well plates for quality control and preparation of libraries using the standard automated KAPA stranded mRNA library preparation protocol (Roche). One sample was lost in transport before library preparation (HG03478 (MSL)) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 ng of pCAG-mCherry were transfected into 80% confluent HEK293T cells in 24-well plates using FuGene6 (Roche) according to the manufacturer’s protocol ...