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Citations for Roche :
151 - 200 of 264 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2024Quote: ... Skin pieces were finely chopped using scissors after fat removal and digested for 90 minutes in media containing Liberase (0.2mg/ml, Roche) and DNAse II (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then incubated for 1.5 hours at 37°C in an orbital shaker (200 rpm) with 1 ml of digestion media containing 0.25 mg/ml Liberase TL (Roche) and 0.25 mg/ml DNase I (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 heads were removed and placed in 173 µL dissecting buffer (Schneider’s media with x1 EDTA-free protease inhibitor cocktail (Roche), x1 RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of the culture was then treated with either 5 µl of MeOH or LMB dissolved in 7:3 MeOH:H2O solution (Roche) at a final concentration of 50 ng/mL for 45 min before imaging.
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bioRxiv - Microbiology 2021Quote: ... xanthus culture was harvested and the pellet resuspended in lysis buffer (50 mM Tris-HCl pH7.6, Protease inhibitor cocktail (Roche)) (1 ml per 80 mg cells) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the culture supernatant after each passage using a High Pure Viral RNA kit (Roche Diagnostics). For deep sequencing of the S1/S2 cleavage site of the viral S gene ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 200 µl of virus-containing cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... cell culture counts were accessed in the count plate on an automated cell counter (Cedex, by Roche, Basel, Switzerland). As there was little or no growth lag after splitting the culture ...
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bioRxiv - Cell Biology 2021Quote: Protein from 2D hPSC-cardiac cell cultures was extracted using RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche). Protein lysate concentration was estimated using a BCA assay (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... resuspended in 20mM Tris-HCl pH 8.0 (30 ml per liter of culture) containing complete EDTA-free protease inhibitor cocktail (Roche) and lysed using a Microfluidizer M-110L (Microfluidics ...
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bioRxiv - Immunology 2020Quote: ... Parasite cultures were confirmed to be free of mycoplasma and acholeplasma using an ELISA-based Mycoplasma Detection Kit (Roche) which contains polyclonal antibodies specific for M ...
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bioRxiv - Microbiology 2020Quote: ... Culture supernatant containing retroviruses was harvested after 48 hours and premixed with DOTAP liposomal transfection reagent (Roche, Basel, Switzerland) prior to spin transfection at 2400 rpm at 32°C for 2 hours into target cells ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from BHI broth cultures using the High Pure PCR template preparation kit (Roche, Potsdam, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Molecular Biology 2022Quote: ... cells from 100 mL exponential phase cultures resuspended in 1.5 mL lysis buffer (buffer W with added cOmplete Mini protease inhibitor cocktail, Roche) were lysed by repeated sonication (10×20 s ...
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bioRxiv - Neuroscience 2022Quote: ... All co-cultures were fixed and post-fixed (before and after incubation with primary rat anti-HA antibody (Roche), respectively ...
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bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Cell Biology 2020Quote: ... L6 cells and cardiomyocytes by incubating them in their respective media containing 2% (w/v) fatty acid-free bovine serum albumin (FAF-BSA; Roche) and 0.4 mM sodium palmitate (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in complete media (DMEM with 10% FBS and 1% antibiotics) supplemented with Collagenase-D (1 mg/mL; Roche) and incubated at 37°C for 30 min with shaking to form a single-cell suspension ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to the 3-4 ml of digestion media containing 0.1 mg/ml liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... P14 CD8+ T cells were activated for 24 h as described above and resuspended in T cell media + 30 U/ml rhIL-2 (Roche) at 2 × 106 cell/ml ...
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bioRxiv - Immunology 2022Quote: ... were placed in 5mL of digest media (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and homogenized in gentleMACS C tubes (Miltenyi Cat#130096334 ...
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bioRxiv - Cell Biology 2021Quote: ... Retinas from adult Long Evans rats were dissected in DMEM/F12 media supplemented with complete protease inhibitors (Roche, Indianapolis, IN) and positioned above a disc of nitrocellulose disc oriented photoreceptor-side down ...
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bioRxiv - Cancer Biology 2021Quote: ... individual primary tumors were dissected from the lungs of KL mice, mechanically dissociated, then digested for 45min in digestion media (10% FBS, pen/strep, 1mg/mLCollagenase/Dispase (Roche) in DMEM ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Immunology 2023Quote: ... in a plate and mechanically disrupted using the back-end of a syringe before addition of 50 μL of a digestion media (dRPMI = naRPMI supplemented with 11x collagenase D (#11088866001, Roche, Woerden ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Bioengineering 2024Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Biochemistry 2023Quote: ... were used to inoculate each well of a 96-well plate containing 196 μL of LB media (supplemented with ampicillin, and 200uM IPTG) The plate was covered with transparent LightCycler480 Sealing Foil (Roche), and grown for 24 h in a Biotek Synergy H1 plate reader at 42 °C with double orbital shaking ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase, Roche). Cells were then washed with complete media and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 15 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 30 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... tumor tissue was incubated at 37°C for 30minutes in FBS free DMEM media containing liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Physiology 2023Quote: ... and then incubated for 45 min at 37°C either in Hanks’ Balanced Salt solution (HBSS) or plain DMEM media supplemented with Liberase HD (200 μg/ml, Roche) and DNase I (40 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and lung lobes were finely minced and incubated in 15 mL of complete media containing 1 mg/mL collagenase (Roche) and 17 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... incubated three days at room temperature in the dark and then moved to selection media (CIM + Hygromycin (40 mg/ml final concentration, Roche) + Timentin (200 mg/ml final concentration ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Genomics 2024Quote: ... The remaining epithelium-depleted tissue was minced and incubated in digestion media (HBSS medium, 0.25 mg/mL Liberase TL (Roche, 5401020001) and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Each half was placed with the dermis layer down in digestion media (RPMI supplemented with 250 μg/ml Liberase TL (Roche) and 125 μg/ml DNase I ...
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bioRxiv - Microbiology 2024Quote: ... who were in the main asymptomatic, provided as pooled (urethral, anorectal, pharyngeal) swab samples using cobas PCR Media Uni Swab Sample Kit (Roche) deriving from a systematic testing strategy within two HIV cohorts (45,46).
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bioRxiv - Bioengineering 2024Quote: ... lung tissue was minced and incubated in 3 mL of serum-free media containing 0.5 mg/mL DNase I (Roche, 10104159001) and 1 mg/mL collagenase type IV (Worthington ...