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Citations for Roche :
151 - 200 of 2286 citations for Plasma membrane calcium transporting ATPase 4 ATP2B4 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... The plasma was separated and stored at −80°C for insulin assay according to manufacturer’s instructions (Roche).
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bioRxiv - Immunology 2021Quote: ... plasma and LN cells were isolated in PBS containing a protease inhibitor cocktail (Roche Diagnostics, Chicago IL) and Ccl19/Ccl21 levels were determined by indirect sandwich of enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Cell Biology 2022Quote: ... islets were pre-incubated for 1 hour in KRBH buffer (described in in vitro calcium imaging) containing 1% w/v BSA (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± agonists in KRBH in a shaking 37°C water-bath (80 rpm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Cell Biology 2023Quote: ... Neuro2A and H4 cells were transfected 24 to 48 h before observation with calcium phosphate or with X-tremeGENE9 (Roche), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... containing 2.5mL pre-warmed digestion buffer consisting of filter-sterilized PBS (with calcium and magnesium) 0.5% bovine serum albumin (BSA) with freshly added DNase I (Roche, 10104159001) at 100μg/mL and Collagenase IV (Worthington ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Membranes were equilibrated (Roche #11093274910) and CDP-Star Substrate (Invitrogen #T2145 ...
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bioRxiv - Microbiology 2020Quote: ... or nylon membranes (11209299001, Roche) were pre-soaked with 10X SSC for 5 min and assembled in dot-blot apparatus (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... transferred to nylon membrane (Roche) and UV-crosslinked using standard manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 150mM NaCl] supplemented with 1% Tween® 20 for 30 min before probing the membrane with either rat monoclonal anti-HA antibody (3F10, Roche) or mouse monoclonal ANTI-FLAG® antibody conjugated to HRP (M2 ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... After incubating in primary antibodies for two hours and DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride, Sigma Aldrich, 10,236,276,001 Roche) for the last ten minutes ...
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bioRxiv - Genetics 2022Quote: ... Overnight incubation with Anti-Dig antibody conjugated to alkaline phosphatase (1:5,000) at 4 °C (no. 11093274910; Roche) was followed by 8 × 30 min washing steps at room temperature with TBST 2 (TBST with 0.1% Tween 20 and 0.05% levamisole–tetramisole ...
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bioRxiv - Neuroscience 2021Quote: ... pre-cleared supernatant (25µl beads and 200µl supernatant, 30min, 4°C) was first incubated with anti-HA antibody 12CA5 (Roche Life Science ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Molecular Biology 2020Quote: ... DIG RNA labelling Kit and Fluorescein (FITC) RNA Labeling Mix (Cat. no. 11685619910, Roche) were used to synthesize probes according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled in vitro transcription of rln3a and sst1.1 probes (11685619910, Roche). The gsc2 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... Sections were incubated with sheep anti-FITC-POD (1:2000, Roche cat# 18 11426346910) or sheep anti DIG-POD (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labeled probes were detected with anti-digoxigenin coupled to fluorescein isothiocyanate (FITC; Roche). Chromosome preparations were counter-stained with 4′,6-Diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... Patient cfDNA was isolated from 2 mL of plasma using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... the plasma cardiac Troponin T (cTnT) level was analyzed on a System Roche Hitachi Cobas (Roche Diagnostics, Switzerland) (55) ...
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bioRxiv - Microbiology 2021Quote: ... Plasma viral load in ART-treated PLWH was measured using the Amplicor HIV-1 monitor ultrasensitive method (Roche).
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bioRxiv - Cell Biology 2022Quote: Troponin T plasma levels were quantified using an electrochemiluminescence immunoassay analyzer “ECLIA” (cobas e 801 immunoanalysis system, Roche) in the hematology laboratory of the Centre Hospitalier Universitaire Vaudois (CHUV) ...
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bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Total murine plasma cholesterol and triglyceride concentrations were measured with colorimetric assays (CHOD-PAP and CPO-PAP, Roche) using a microplate reader (Infinite F200 PRO ...
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bioRxiv - Pathology 2023Quote: ... mice were weighed and blood was harvested from the facial vein to monitor plasma cholesterol (CHOL2, Roche Diagnostics), and six hour fasting blood glucose was measured four weeks into the experiment and four weeks prior to euthanasia (tail tip bleed readings by Accu-Chek ...
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bioRxiv - Microbiology 2024Quote: HIV-1 RNA levels were measured in cell-free CSF and plasma at each site using the ultrasensitive Amplicor HIV-1 Monitor assay (versions 1.0 and 1.5; Roche Molecular Diagnostic Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with ice-cold PBS (w/o calcium and magnesium) and lysed immediately in RIPA-Buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: Remaining isolated mitochondria from our calcium and respiratory assays were pelleted and lysed in RIPA buffer supplemented with phosphatase inhibitors (Roche, 4906837001) and protease inhibitors (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were blocked in 5% (w/v) nonfat dry milk and probed with anti-HA antibodies (Roche 11867431001; 1:10,000 dilution), followed by horseradish peroxidase-coupled secondary anti-rat antibody (Sigma-Aldrich A9037 ...
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bioRxiv - Genetics 2021Quote: ... Then they were incubated overnight at 4°C with primary antibodies diluted in a dilution buffer (0.5% blocking reagent (Roche), 2% fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
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bioRxiv - Plant Biology 2020Quote: ... roots were incubated overnight at 4°C in anti-myc-mouse primary antibody (1:250, Roche in blocking solution), washed 3 times in PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Neuroscience 2021Quote: ... Tween-20 0.3%) for 1 hour and finally incubated overnight at 4°C with anti-Dig-POD antibody (Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was incubated at 4°C overnight with 20 μl anti-HA antibody coated beads (Roche, Rotkreuz, Switzerland) at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with anti-digoxigenin-alkaline phosphatase antibodies (1:5000; Roche; 11093274910; AB_514497) diluted in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Plant Biology 2024Quote: ... onto a Hybond–N membrane (Roche). The membrane was auto-crosslinked at 120,000 μJ in a Stratalinker 1800 (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to PVDF membranes (Roche). The membranes were blocked in 5% non-fat milk (Difco ...
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bioRxiv - Cell Biology 2023Quote: ... transferred to PDF membrane (Roche, 03010040001) and then visualized by Western blotting ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... minced finely and digested in pre-warmed PBS (with calcium and magnesium) with 2 mg/ml of collagenase A (Roche, Basel, Switzerland) for 1h on a rotating wheel at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured on Cobas Integra 400 Clinical Analyzer (Roche Diagnostics).
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bioRxiv - Genetics 2021Quote: ... Isolation of cfDNA from 2 mL of plasma was performed using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... resuspended in RPMI-1640 (Fisher) supplemented with 10% Fetal Bovine Plasma (FBS) and 20 U/mL deoxyribonuclease I (Roche), concentrated by centrifugation (300 x g for 15 min at 20°C) ...