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Citations for Roche :
151 - 200 of 2081 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The soluble nuclear fraction was collected by incubation with the nuclear buffer (10mM HEPES pH7, 200mM NaCl, 1mM EDTA, 0,5% NP-40) supplemented with protease inhibitors cocktail (Roche) for 10min on ice and centrifuge at 14000rpm for 2min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The nuclear pellets were resuspended in nuclear lysis buffer (50mM Tris-HCl pH 8.0, 10mM EDTA, 1% SDS, 1mM PMSF, cOmplete Protease Inhibitor Cocktail (Roche)) and vortexed at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 M Urea loading dye was supplemented with complete protease inhibitor (Roche). 100 μl Urea loading dye were used to resuspend cell pellet after NaOH treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR was performed with SYBR Green I dye master mix (Roche) and a CFX connect Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The amplicon was detected by EvaGreen Dye using LightCycler 480 instrument (Roche). PCR conditions were 5 min at 95 °C ...
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bioRxiv - Biophysics 2021Quote: ... at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche, Basel, Switzerland) coated coverglass surface at the other end through digoxigenin-antibody interactions ...
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bioRxiv - Genomics 2020Quote: ... were labelled with biotin 16-dUTP using a nick translation kit (Roche Diagnostics, Basel, Switzerland) and hybridized to metaphase chromosomes overnight at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... Probe DNA was nick-translated with either Digoxigenin-11-UTP or Biotin-16-UTP (Roche) using DNase I (Roche ...
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bioRxiv - Genomics 2022Quote: ... The 18S rDNA probe was labelled either with biotin-16-dUTP (Roche Diagnostics, Basel, Switzerland) or DNP-11-dUTP (Jena Bioscience ...
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bioRxiv - Plant Biology 2022Quote: ... The 5’-end biotin probes were generated using a DIG Gel Shift Kit (Roche, China) (Supplemental Table S8) ...
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bioRxiv - Genomics 2024Quote: ... Specific Hydrangea satDNA probes were labeled indirectly by PCR with biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Medium calcium content was measured by photometrical absorption measurement of calcium EDTA complex using a Calcium Gen.2 kit (Roche, Germany). After 7 days ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibodies were used to detect green fluorescent protein (GFP) (Cat#11814460001, clones 7.1 and 13.1, Roche). Rabbit polyclonal anti-Cdc42p antibodies were provided by Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... The enriched FLC-Venus protein was detected by western blot assay with the antibody anti-GFP (11814460001, Roche). Signals were visualized with chemiluminescence (34095 ...
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bioRxiv - Pathology 2024Quote: ... Pre-cleared lysates (containing 1 mg of protein) were then incubated with mouse monoclonal anti-GFP antibodies (Roche) coupled to G-protein beads for 3 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... PCMD-1 transgene proteins with GFP-tag were probed with a GFP antibody (mouse monoclonal 1:600, Roche) or α-Tubulin antibody (mouse monoclonal 1:7500 ...
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bioRxiv - Developmental Biology 2024Quote: ... Localization of HA tagged proteins were analyzed with an anti-HA tag antibody (Roche, clone 3F10, 1 : 500) and Alexa Fluor coupled secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: To prepare nuclear extracts all procedures were done at 4°C and buffers supplemented with 1x protease inhibitors (Roche, 4693132001), 15 μM MG-132 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
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bioRxiv - Genomics 2024Quote: ... in nuclear lysis buffer (50 mM Tris-HCl [pH 7.5], 10 mM NaCl, 0.2% NP-40, 1x Roche protease inhibitors) at 4
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Single-stranded Xist riboprobe was generated by in vitro transcription using biotin-16-UTP (Roche, 11388908910) and MAXIscript SP6 kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... BACPAC Resources) were labelled with digoxigenin-dUTP or biotin-dUTP using a nick translation kit (Roche), denatured (95°C ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of plasmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910), digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910) or digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Genomics 2022Quote: ... limited PCR cycles were used to amplify the designated probe sequences from the oligo complex pool with kapa HiFi HotStart Polymerase (Roche, Cat. KK2502). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... F0425)/500 µg protein with protein A-agarose (Roche). Fished-out FGFR3 protein was eluted and then denatured at 95°C for 10 minutes in NuPAGE LDS sample buffer (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei pellet was then resuspended and incubated in 1 mL of nuclear lysis buffer (50mM Tris-HCL pH8.0, 100mM EDTA, 1% SDS, 1mM PMSF, SIGMAFAST™ protease inhibitor cocktail, and Roche PhoStop) on ice for 30 min and 3 freeze-thaw cycles to release chromatin ...
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bioRxiv - Molecular Biology 2020Quote: ... nuclei were pelleted by centrifugation at 2500g for 5 minutes and resuspended in 880ul Nuclear Lysis Buffer (50mM Tris HCl, 10mM EDTA, 1% SDS, 1X protease inhibitors (Roche, 04693124001)) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
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bioRxiv - Plant Biology 2022Quote: ... and the interaction between AtRsmD and the selected proteins was determined by immunoblot analysis with a mouse anti-GFP monoclonal antibody (1:5,000; Roche) and a mouse anti-Myc monoclonal antibody (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto nitrocellulose gels and probed with following antibodies: rat α-HA (clone 3F10; Roche Applied Sciences), rabbit α-mNeonGreen (Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with a 1µg/ml solution of the DNA-dye DAPI (Roche) and mounted with the Slowfade antifade reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). The labeled nuclei were purified using FACS (BD Aria ...
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bioRxiv - Cell Biology 2023Quote: ... uuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). All GFP+ Hoechst stained myonuclei were purified using FACS (BD Aria ...
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bioRxiv - Cell Biology 2024Quote: ... nuclei were labeled with Hoechst dye and 0.2 U/μL Protector RNase inhibitor (Roche). All Hoechst stained myonuclei were purified using FACS (BD Aria ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA probes were labelled by nick translation with either biotin-16-dUTP or digoxigenin-11-dUTP (Roche). For simultaneous IF and RNA FISH ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The labelling nucleotide mix contained either biotin-16-dUTP or digoxigenin-11-dUTP (both Roche, Mannheim, Germany). Due to its long size ...
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bioRxiv - Genomics 2021Quote: ... rp49 5’-TAATACGACTCACTATAGGGCAGTAAACGCGGTTCTGCATG-3’ and 5’-CAGCATACAGGCCCAAGATC-3’) were transcribed using the Biotin RNA Labeling Mix (Roche) and T7 polymerase (Promega).
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Genetics 2023Quote: Cell migration assays were performed with the xCELLigence Biosensor System using specifically designed 16-well plates equipped with membranes having 8-μm pores (CIM-plate 16; Roche Diagnostics). Cells in serum-free medium were seeded in the upper chambers ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Cell Biology 2020Quote: ... post-nuclear lysates pre-cleared using CL-4B Sepharose beads followed by immunoprecipitation with anti-HA-antibody (12CA5) and Protein G agarose (Roche). Beadbound radiolabelled substrates were resuspended in 2 x Laemmli buffer (+20 mM DTT) ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged for 15 minutes at 13000 rpm and then the supernatants were incubated mouse monoclonal anti-huntingtin antibody MAB2166 (MilliporeSigma) conjugated Protein G Agarose (Roche) at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: Surface expression of HA-C99 and cc-del(X) fusion proteins was measured with monoclonal HA-antibody (Roche 1:100) by flow cytometry as described previously (Staerk et al. ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...