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Citations for Roche :
1901 - 1950 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Primary fibroblast cultures were obtained by subjecting small pieces of mouse ear tissue to a 30-minute digestion with Collagenase/Dispase (Roche) at 37°C under agitation ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... the same procedure was used using different lysis (50 mM HEPES pH 7.4, 100 mM NaCl, 10% glycerol, 1 mM DTT, 1 mM ATP, 2 mM PMSF, 1 Roche tablet per 50 mL) and storage (50 mM Tris pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by incubating cells in RIPA lysis buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP40, 1% deoxycholate, 0.1% SDS, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF) on ice for 30 minutes and cell lysate was clarified by centrifugation at high speed (15 000 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... 1% NP-40, 150 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0 and Roche complete protease inhibitor EDTA-free) and acid-washed glass beads using a Precellys machine (6 × 30 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... 1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM Na3VO4×2H2O, 5 µM pepstatin A, 10 µM leupeptin, 2X Roche protease inhibitor cocktail), 200µl glass beads were added ...
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bioRxiv - Genetics 2022Quote: ... 5 mM EDTA. 0.2% Triton X-100, 1 mM PMSF, 1% Glycerol, 1 pellet/50 ml EDTA free Protease inhibitor [Roche] and 25 μM MG132). 100 μl diluted mixture was used as input ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently lysed (1 mM NaF, 1 mM Na3VO4, 10 nM calyculin A, 1 mM PMSF, 1 mg complete EDTA-free protease inhibitor cocktail [Roche] in RIPA buffer), scraped ...
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bioRxiv - Physiology 2022Quote: ... cell pellets were resuspended in RIPA Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% IGEPAL CA-630, 1% deoxycholate, 1 mM DTT, DNase, and Roche cOmplete Protease Inhibitor Cocktail) and lysed via sonicating water bath ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, 1X EDTA-free protease inhibitor cocktail [#04693132001, Roche], and 0.5% sodium deoxycholate) and collected using a cell scraper ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2% Triton X-100, 1 mM EDTA, 1 mM dithiothreitol, 1 mM NaF, 10 mM β-glycerophosphate, 0.1 mM Na3VO4, 1x Roche cOmplete protease inhibitors). Protein lysates (12 µg per lane ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples (containing 20 μg of total protein) were mixed with loading buffer and separated on 12 % SDS-PAGE gels and transferred to PVDF membranes (Roche, Switzerland). After blocking with Tris-buffered saline with 0.1% Tween 20 and 5 % nonfat dry milk ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting yeast extracts were used for immunoblot analysis using anti-Myc (MBL, Woburn, MA, United States) for bait proteins and anti-HA (Roche, Switzerland) for prey proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2020Quote: Western blotting was performed as previously reported.[26] Protein extracts were prepared using RIPA buffer containing EDTA protease (Roche Applied Science) and phosphatase inhibitors (Phostop Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting yeast extracts were used for western blot analysis using anti-Myc HRP-DirecT (MBL, Woburn, MA, United States) for bait proteins and anti-HA (3F10)-HRP (Roche, Switzerland) for prey proteins.
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bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
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bioRxiv - Systems Biology 2019Quote: ... The cross-linked proteins-RNA were cut directly from the gel and incubated with 160 μg of Proteinase K (Roche, 3115801001) in 600 μl wash buffer 2 supplemented with 1% SDS and 5 mM EDTA at 55°C for 2-3 hours with mixing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were extracted from RAW264 using radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Complete Mini, Roche Diagnostics GmbH, Mannheim, Germany) and phosphatase inhibitors (PhosSTOP EASYPack ...
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bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Hand dissected ovaries from 100-150 flies (depending on the expressed protein) of appropriate genotypes were lysed in RIPA buffer supplemented with Complete Protease Inhibitor cocktail (Roche, 11836170001) and 20 mM NEM ...
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bioRxiv - Neuroscience 2023Quote: Proteins were detected by Western blot analysis after separating 30–50 μg of total protein lysate prepared in RIPA buffer with protease inhibitor cocktail (cOmplete™, Roche) on a 4– 15% Mini-PROTEAN® TGX™ Precast Gel (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Molecular Biology 2023Quote: ... containing either single (for the IgG screen) or serial dilutions (for full IgG multi-point kinetics) of HEL protein (Roche #10837059001). For measurement of Fab binding kinetics ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg nuclear lysate was adjusted to 500 uL in Protein Incubation Buffer (PIB+++; 150mM NaCl, 50 mM Tris pH 8.0, cOmplete protease inhibitor (Roche, cat 04693159001), 0.25% NP-40 and 1 mM DTT) ...
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bioRxiv - Cell Biology 2019Quote: ... Rat monoclonal anti-HA (1:50 for IF and 1:1000 for IB; Roche); mouse monoclonal anti-α-tubulin (1:200 for IF and 1:2500 or 1:10,000 for IB ...
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bioRxiv - Molecular Biology 2019Quote: ... The washed cellular fraction was then resuspended in 1 × PBS and 1 × cOmplete (Roche). 150 μL of supernatant (of each sample ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 protease inhibitor cocktail tablets (1 tablet in 10mL of lysis buffer, Roche (#11836153001)) ...
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bioRxiv - Plant Biology 2021Quote: ... PMSF 1 mM and 1 protease inhibitor tablet (Roche cOmplete EDTA free cat# 05056489001)/50 ml) ...
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bioRxiv - Microbiology 2020Quote: ... 1% Triton X-100) containing 1 tablet/50 ml of protease inhibitor cocktail (Roche), transferred to a tube and incubated for 15 min at 4°C on a wheel ...
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bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x protease inhibitor cocktail (cOmplete ULTRA Tablets, Mini, EDTA-free, Roche; 1:10), pH 8.5 ...
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bioRxiv - Microbiology 2021Quote: ... incubating in 1% blocking solution with 1:10,000 α-Digoxigenin-AP Fab fragments (Roche) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg/ml-1 DNase I and 1 protease inhibitors (cOmplete EDTA-free, Roche) for each gram of pellet ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% NP40 supplemented with 1 tablet of protease inhibitor (Complete ultra mini-tablet, Roche) and phosphatase inhibitor (PhosphoStop tablet ...
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bioRxiv - Microbiology 2022Quote: ... 10U mL-1 DNase 1 and protease inhibitors – Complete Protease Inhibitor Cocktail Tablet (Roche)) ...