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Citations for Roche :
1901 - 1950 of 3367 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Glucose production rates were determined at 2 h using an Accu-Chek ®Aviva glucometer (Roche Diagnostics Scandinavia AB, Sweden). Monosaccharide (arabinose ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 4 ml of HBSS (without Ca2+ or Mg+) and 10 ml of collagenase solution [2 mg/ml collagenase H (11074059001; Roche), 0.1 mg/ml DNaseI (10104159001 ...
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bioRxiv - Microbiology 2023Quote: ... coli cell pellets was carried out by resuspending pellets in 50 mL of lysis buffer (50 mM NaPO4, 300 mM NaCl, 20 mM imidazole, 10 mM 2-mercapoethanol, protease inhibitor (Roche), DNAse (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... glands were minced into paste and incubated in 5 mL DME/F-12 medium (HyClone, #SH30023.1) with 2 mg/mL collagenase A (Roche #10103578001), 100 units/mL hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Tumors excised at day indicated in each figure were disaggregated mechanically and enzymatically with 2 mg/mL collagenase IV and 50 U/mL DNase I (Roche).
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bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
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bioRxiv - Neuroscience 2023Quote: Mouse brains at 5W were isolated and homogenized in homogenization buffer [(0.32 M sucrose, 10 mM HEPES, 2 mM EDTA and 1× complete protease inhibitor cocktail (Roche Diagnostics), pH 7.4)] ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in 4x pellet volume of sucrose buffer (10 mM HEPES/KOH pH 7.5.; 2 mM MgAc; 250 mM Sucrose, 1x Protease inhibitor cocktail [Roche, Germany]) and lysed with ~50 strokes in a tight-fit dounce homogenizer ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
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bioRxiv - Genomics 2022Quote: ... Re-purified circular fragments were amplified by PCR with View-Point specific primers (see Supplementary Table 2) using the Expand Long Template PCR System (Roche). Resulting amplicons were purified with a 0.8× ratio of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... the cell pellets resuspended in lysis buffer (purification buffer 50 mM Tris pH 8, 50 mM NaCl, 2 mM DTT; + cOmplete EDTA-free Protease Inhibitor (Roche)) and lysed by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated for 1 hour in TBST and then in Blocking Buffer (2% blocking reagent in maleic acid buffer pH 7.5 (Roche, #11096176001) and 10% sheep serum (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The suspension medium was carefully removed and replaced with fresh PDB supplemented with 2× protease inhibitor (Mini Protease Inhibitor Cocktail, Roche), 0.5 mM or 2 mM PMSF (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended and homogenized in buffer B supplemented with 2 mM MgCl2 and 1x protease inhibitor (Roche). The protein was extracted by incubating with 1% GDN (Anatrace ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Cell Biology 2023Quote: ... 2 M urea, 0.5% SDS, 1 mM EDTA pH8.0, 1 mM DTT, and 1× protease inhibitor cocktail [Roche, cat#4693132001]). Biotinylated RBPs were captured by affinity purification ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 4°C overnight with the primary antibody mouse IgG1 Anti-Tbx6 (home-made, clone A83, dilution 1:500) in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
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bioRxiv - Neuroscience 2023Quote: Hippocampi were homogenized in 500µl of RIPA medium lysis buffer (Beyotime, #P0013E-2) in the presence of protease inhibitor cocktail (Roche, #11836170001). Samples were centrifuged at 10,000 × g for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 mL dissociation buffer (RPMI-1640 (Cytiva) with 25 mM HEPES and 10 µg/mL gentamicin sulfate) supplemented with 2 mg/mL Collagenase-D (Roche) and 100 U DNaseI (Roche) ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
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bioRxiv - Cell Biology 2024Quote: ... then with 1 ml of Western blot buffer (2% SDS, 5% Glycerol, 50 mM Tris-HCl, 0.2 M EDTA + cOmplete protease inhibitor cocktail tablet (Roche, 11697498001) + PhosSTOP (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies used were prepared in 2% BSA (diluted in PBS) and used at the following concentrations: rat monoclonal anti-HA (1:1000, 3F10 Roche), mouse monoclonal anti-myc (1:100 ...
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bioRxiv - Systems Biology 2024Quote: The eluted samples were used as a template for a PCR reaction for amplifying Tn5 tagmented fragments and sample barcoding (2× Kappa HIFI [Roche] ...
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bioRxiv - Developmental Biology 2024Quote: ... The agarose was suspended in dilution buffer for IPs from cell lysates and in a 2 : 1 lysis buffer : dilution buffer mix for IPs from EC fractions and supplemented with cOmplete Protease Inhibitor Cocktail (Roche). Of the 130 µl EC fraction/cell lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’/3’RACE Kit (Roche) was used to amplify the RNA obtained and the final DNA was sequenced to obtain the +1 nucleotide of str ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Cell Biology 2020Quote: ... 1mM PMSF) containing one tablet of EDTA-free Complete Protease Inhibitors (Roche). The cell pellet was frozen in liquid nitrogen and ground with a freezer mill (6775 Freezer Mill) ...
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bioRxiv - Cell Biology 2021Quote: ... with KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems, MI). NCX ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 40 U/ml DNAse and one EDTA-free protease-inhibitor tablet (Roche)] ...
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bioRxiv - Biochemistry 2020Quote: ... and one tablet of protease cocktail inhibitor (Roche Diagnostics, Indianapolis, IN, USA) per 25 ml of buffer ...
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bioRxiv - Microbiology 2021Quote: ... using the KAPA SYBR FAST One-Step qRT-PCR kit (Kapa Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 mM DTT) supplemented with one Complete mini protease inhibitor tablet (Roche) and disrupted by French Press ...
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bioRxiv - Biophysics 2020Quote: ... one flow cell volume of 100 μg/ml anti-digoxigenin (Roche, Switzerland) in 1x PBS is introduced ...
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bioRxiv - Molecular Biology 2022Quote: One tablet of ‘Roche cOmplete EDTA-free protease inhibitor Cocktail’ (Roche, 11873580001) was either added directly to 20 ml of protein purification buffer or else dissolved in 1 ml of water to make 20X stock.
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bioRxiv - Microbiology 2022Quote: ... 10% glycerol) and supplemented with one tablet of protease inhibitor cocktail (Roche). The suspension was adjusted to 0.1 mg/ml lysozyme (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 10% glycerol) and supplemented with one tablet of protease inhibitor cocktail (Roche). The suspension was adjusted to 0.1 mg/ml lysozyme (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 10% glycerol) and supplemented with one tablet of protease inhibitor cocktail (Roche). The suspension was adjusted to 0.1 mg/ml lysozyme and incubated on ice for 30 min ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... supplemented with one tablet of protease inhibitor cocktail (cOmplete EDTA-free, Roche) per pellet ...