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Citations for Roche :
1851 - 1900 of 6330 citations for Oxytocin ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated at 37oC in a staining solution containing nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) for 20-24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 0.1 % Triton X-100, complete protease inhibitor cocktail [Roche] added fresh) for 10 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting cDNAs were amplified with primers DP3 and DP5 (5’-GTTCAGAGTTCTACAGTCCGACGATC-3’, 0.5 μM) and KAPA Hifi HotStart DNA polymerase (Roche, KK2601) to the optimal amplification point ...
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bioRxiv - Pathology 2023Quote: ... Bound alkaline phosphatase was visualized with nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP; 11681451001, Roche). The reaction was stopped by incubation in buffer 4 (10 mM Tris and 1 mM EDTA ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.44 M sucrose, 1.25 % Ficoll, 2.5% Dextran, 10 mM MgCl2, 0.5% Triton X-100, 5 mM DTT, 1x Roche protease inhibitor mixture), filtered through two layers of Miracloth ...
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bioRxiv - Immunology 2023Quote: Whole lung single cell suspensions were prepared by harvesting lung lobes into 5 ml HBSS with 40µl Liberase Tm (0.1 U/ml, Roche, Cat# 5401127001) and 20µl DNAse 1 (10mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals were detected by 4-nitro-blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Roche Diagnostics) in a humidified container for 72 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Biochemistry 2023Quote: ... 100 mM NaCl, 20 mM Imidazole pH 7.5, 3 mM MgCl2, 100 µM EDTA, 5 mM β-Mercapoethanol, 20 µM GDP, Roche cOmplete protease inhibitor cocktail and DNAse I ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate; Roche) until the staining appeared (overnight or up to 48 h) ...
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bioRxiv - Molecular Biology 2024Quote: ... and qPCR was carried out in 5 µL multiplexed reactions and 384-well format using the LightCycler 480 Instrument II (Roche). Final concentration of primers are 0.5 µM and probes are 0.25 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 µl of diluted cDNA was used for each qPCR reaction carried out using KAPA SYBR FAST master mix (Roche). Relative expression levels for each gene were determined based on the 2−ΔΔCT method normalizing against 7SK RNA.
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bioRxiv - Plant Biology 2024Quote: The pulverized tissue was incubated in 30 mL of buffer 1 (400 mM sucrose, 10 mM Tris-HCl pH 8.0, 10 mM MgCl2, 5 mM β-mercaptoethanol, 1 mM PMSF, 1× Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were ligated by adding 15 µL of the ligation mix (3 µL T4 Ligase Buffer 10X, 0.5 µL T4 ligase (5 U/µL, Roche, 10799009001), 0.25 µl of x-Gene Stubby Adapter 50 mM (IDT) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was discarded, and 5 mL of Hank’s Balanced Salt Solution (Ca-, Mg-) (gibco) buffer containing liberase (Roche, #05401119001) (50 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 1 mM ATP, 1% IGEPAL, 5% glycerol, 3 U/ml Benzonase and Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Cell Biology 2024Quote: ... followed by an 8-minute perfusion with myocyte digestion buffer containing 5 mg of liberase dispase high DH enzyme (Roche) per digestion ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... The reactions were performed in triplicates in white FrameStar® 384 PCR plates (4titude) on a LightCycler® 480 system (Roche Diagnostics, Meylan, France) and gene expression analysis was performed with the use of qBase [40] ...
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bioRxiv - Bioengineering 2021Quote: ... expanded leaves of 7-week old hybrid aspen plants were sampled with a biopsy punch (4 mm diameter) and loaded into wells of 96-well plate (Roche LightCycler® 480 System) containing 100 μL of water (one sample per well) ...
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bioRxiv - Immunology 2019Quote: All quantitative PCR (qPCR) reactions were performed in duplicates and conducted in 96 well plates on LightCycler® 480 system (Roche Diagnostics, Mannheim, Germany). Each reaction consisted of a total amount of 12 µL divided into 6 µL LightCycler 480 SYBR Green I Master (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded in conditioned medium in triplicate in 6 cm plates and allowed to attach for 4 h before treatment with mitomycin C (Roche 10 107 409 001) or olaparib (Selleckchem AZD2281 ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions (10 µl) were set up in Light cycler 480 plates using the LightCycler 480 probe master mix (Roche, Welwyn Garden City UK) according to the manufacturer’s instructions (Tables 2) ...
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bioRxiv - Cancer Biology 2021Quote: ... The Fast Start TaqMan® Probe Master Kit (Roche) additionally contains Taq DNA Polymerase ...
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bioRxiv - Cell Biology 2019Quote: ... and quantified using Kapa Library Quantification Kit (Kapa Biosystems). Each library was sequenced in one lane of HiSeq4000 (Illumina ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were first purified with a midiprep kit (Roche) and were transfected into HEK293T cells with a five-plasmid system to produce lentivirus with a very high titer of ~107 CFU/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... cRNA probes were generated by Digoxin labeling kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were quantified by KAPA Library Quantification Kit (Roche), normalized ...
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bioRxiv - Molecular Biology 2021Quote: ... libraries were prepared with Kapa Hyper Prep kit (Roche) according to the manufacturer’s instructions with 12 amplification cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were incubated in the Fluorescein TUNEL kit (Roche) for 1 hour at 37°C with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... and visualized using the Ultraview detection kit (Roche Diagbostics) on a Benchmark XT autostainer (Ventana ...
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bioRxiv - Developmental Biology 2020Quote: ... or using the KAPA SYBR FAST kit (KAPA Biosystems) on a Bio-Rad CFX96 machine ...
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bioRxiv - Developmental Biology 2022Quote: ... and transcribed with a DIG-labelling Kit (Roche, #11277073910) using T3 polymerase (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... Probes were labelled using DIG-DNA labelling KIT (Roche). For detection five probes were pooled after labelling ...