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1851 - 1900 of 2545 citations for Mouse anti Ross River virus E2 Glycoprotein D7 since 2019

• Retinoic acid, an essential component of the RP organizer, promotes the spatio-temporal segregation of dorsal neural fates

  Dina Rekler, et al., bioRxiv - Developmental Biology 2024

  Quote: ... The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche, 11093274910). AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT ...
• A Toxoplasma gondii O-glycosyltransferase that modulates bradyzoite cyst wall rigidity is structurally and functionally distinct from host homologues

  Pranav Kumar, et al., bioRxiv - Biochemistry 2023

  Quote: ... the blots were probed with HRP-conjugated rat anti-HA antibody (clone 3F10, Roche) at a 1:1000 dilution and incubated overnight ...
• Notch signalling plays a critical role in patterning the ventral mesoderm during early embryogenesis in Drosophila melanogaster

  Marvel Megaly, et al., bioRxiv - Developmental Biology 2023

  Quote: Anti-sense probes were synthesized using the DIG and Biotin-RNA labelling kit (Roche, 11277073910 and 11685597910 ...
• Rtf1-dependent transcriptional pausing regulates cardiogenesis

  Adam D. Langenbacher, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Probes were detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase (Roche 11093274910). The zebrafish antisense RNA probes used in this study include myl7 ...
• Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent pain

  Sebastián Marty-Lombardi, et al., bioRxiv - Neuroscience 2022

  Quote: ... Sections were incubated with sheep anti-FITC-POD (1:2000, Roche cat# 18 11426346910) or sheep anti DIG-POD (1:1000 ...
• Phenotypical Rescue of Bmp15 Deficiency by Mutation of Inhibin α (inha) Provides Novel Clues to How Bmp15 Controls Zebrafish Folliculogenesis

  Yue Zhai, et al., bioRxiv - Developmental Biology 2022

  Quote: ... The hybridization signal was detected by horseradish peroxidase (HRP)-conjugated anti-fluorescein antibody (Roche) with TSA-fluorescein ...
• VCF1 is an unconventional p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates

  Ann Schirin Mirsanaye, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Immunoprecipitation (IP) of HA-tagged proteins was performed using Anti-HA Affinity matrix (Roche) under denaturing conditions ...
• Cytoplasmic redox imbalance in the thioredoxin system activates Hsf1 and results in hyperaccumulation of the sequestrase Hsp42 with misfolded proteins

  Davi Gonçalves, Sara Peffer, Kevin A. Morano, bioRxiv - Cell Biology 2023

  Quote: ... blocked in 5% nonfat dry milk and proteins detected using monoclonal anti-GFP (Roche), monoclonal anti-PGK (Invitrogen) ...
• The molecular basis of macrochaete diversification highlighted by a single-cell atlas of Bicyclus anynana butterfly pupal forewings

  Anupama Prakash, Emilie Dion, Antónia Monteiro, bioRxiv - Developmental Biology 2023

  Quote: ... Probe detection was by tissue incubation in 1:3000 anti-DIG alkaline phosphatase (Roche) in block buffer for 1 hour ...
• Bats possess the anatomical substrate for a laryngeal motor cortex

  Alexander A. Nevue, et al., bioRxiv - Neuroscience 2023

  Quote: ... Slides were then incubated in alkaline phosphatase-labeled anti-DIG antibody (Roche; 1:600) in TNB for 2 h ...
• Shared GABA transmission pathology in dopamine agonist- and antagonist-induced dyskinesia

  Yoshifumi Abe, et al., bioRxiv - Neuroscience 2023

  Quote: ... they were incubated with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:5000; Roche) for 90 minutes at room temperature ...
• Sequential Switching Through IgG1 is Redundant for Allergic Reactivity and Memory to Allergens

  Joshua F.E. Koenig, et al., bioRxiv - Immunology 2023

  Quote: ... incubated with 50 μl of anti-digoxigenin POD fragments (Roche, 11 633 716 001) diluted 1:5000 in 0.3% BSA PBS for 1 hour at RT ...
• Deep spatial proteomic exploration of severe COVID-19-related pulmonary injury in post-mortem specimens

  Yiheng Mao, et al., bioRxiv - Systems Biology 2023

  Quote: ... rabbit anti-furin antibody (1:800, 18413-1-AP, proteintech) and DAPI (10236276001, Roche). The bright-field and immunofluorescent images were acquired using a Pannoramic MIDI system (3DHISTECH ...
• The cytochrome b carboxyl-terminal region is necessary for mitochondrial Complex III assembly

  Daniel Flores-Mireles, et al., bioRxiv - Biochemistry 2022

  Quote: ... transferred to PVDF membrane and inmunodecorated with antibodies against: anti-HA (Roche Applied Science), anti-Cytb (García-Guerrero et al. ...
• Proximity interaction analysis of the Plasmodium falciparum putative ubiquitin ligase PfRNF1 reveals a role in RNA regulation

  Afia Farrukh, et al., bioRxiv - Cell Biology 2023

  Quote: The following antibodies were used in the study: rat anti-HA (Roche, Basel, Switzerland), rabbit anti-HA (Sigma-Aldrich) ...
• KDM2B regulates hippocampal morphogenesis by transcriptionally silencing Wnt signaling in neural progenitors

  Bo Zhang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Slides were incubated with 1:5,000 dilution of anti-digoxigenin-AP conjugated antibody (Roche) overnight at 4°C ...
• Deciphering divergent trypanosomatid nuclear complexes by analyzing interactomic datasets with AlphaFold2 and genetic approaches

  Elvio Rodriguez Araya, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The membrane was then stripped and incubated with rat anti-HA primary antibody (Roche) at 1/2000 dilution in PBST 5% milk and washed 3 times ...
• Nuclear cytoophidia assembly represses transcriptional activity to control skeletal development and homeostasis

  Cheng Xu, et al., bioRxiv - Cell Biology 2023

  Quote: ... embryos were incubated with anti-DIG-AP Fab fragments antibody (Roche, 11093274910, 1:2000) for overnight ...
• Comparative cytogenetics of kenaf (Hibiscus cannabinus L.) breeding lines reveal chromosomal variability and instability

  Nii-Ayi Ankrah, et al., bioRxiv - Plant Biology 2023

  Quote: ... Digoxigenin-labeled probes were detected with anti-digoxigenin coupled to fluorescein isothiocyanate (FITC; Roche). Chromosome preparations were counter-stained with 4′,6-Diamidino-2-phenylindole (DAPI ...
• NOC1 is a Direct MYC Target, and Its Protein Interactome Dissects Its Activity in Controlling Nucleolar Function

  Valeria Manara, et al., bioRxiv - Cell Biology 2023

  Quote: ... membranes were incubated overnight with primary antibodies: rat monoclonal anti-HA (1:1000, ROCHE), or Actin5c (1:200 ...
• A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing

  Nina Lukhovitskaya, et al., bioRxiv - Plant Biology 2024

  Quote: ... and then detected with anti-digoxigenin antibody conjugated to alkaline phosphatase (Roche Diagnostics GmbH) and Western Blue stabilised substrate (Promega).
• Cell-specific polymerization-driven biomolecular condensate formation fine-tunes root tissue morphogenesis

  Jianbin Su, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Protein was detected with 1:3,000 horseradish peroxidase-conjugated anti-HA (Roche, cat#12013819001) or anti-GFP (Invitrogen ...
• EBF1 limits the numbers of cochlear hair and supporting cells and forms the scala tympani and spiral limbus during inner ear development

  Hiroki Kagoshima, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and incubated with a 1:4000 dilution of Anti-Digoxigenin-AP Fab fragments (Roche) at 4 ℃ overnight.
• High glucose treatment induced nuclei aggregation of microvascular endothelial cells via foxo1a-klf2a pathway

  Xiaoning Wang, et al., bioRxiv - Cell Biology 2024

  Quote: ... The hybridization result was detected with anti-DIG-AP antibody (1:2000, Roche, 11093274910) and NBT/BCIP (1:500 ...
• Genomic loss of GPR108 disrupts AAV transduction in birds

  Alexander A. Nevue, et al., bioRxiv - Genomics 2024

  Quote: ... Sections were blocked an incubated in anti-DIG-AP Fab fragments (1:600; Roche) for two hours and incubated in BCIP/NBT chromogen (PerkinElmer ...
• Early Embryonic Development of the German Cockroach Blattella germanica

  Ariel Bar-Lev Viterbo, et al., bioRxiv - Developmental Biology 2024

  Quote: ... 4 h in 1:1500 solution of anti-digoxigenin antibody (Roche, cat no. 11333089001):blocking solution ...
• Drosophila model to clarify the pathological significance of OPA1 in autosomal dominant optic atrophy

  Yohei Nitta, et al., bioRxiv - Pathology 2024

  Quote: ... The following antibodies were used: rat anti-HA (3F10, 1:400; Roche, Switzer land), mouse anti-Chaoptin (24B10 ...
• Spatial resolution of transcriptomic plasticity states underpinning lethal morphologies in lung adenocarcinoma

  Hannah L Williams, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by DISCOVERY OmniMap anti-Ms HRP secondary antibody (Roche Tissue Diagnostics, ref. 05269652001) for 12 minutes ...
• Spatial resolution of transcriptomic plasticity states underpinning lethal morphologies in lung adenocarcinoma

  Hannah L Williams, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by DISCOVERY OmniMap anti-Ms HRP secondary antibody (Roche Tissue Diagnostics, ref. 05269652001) for 12 minutes ...
• Spatial resolution of transcriptomic plasticity states underpinning lethal morphologies in lung adenocarcinoma

  Hannah L Williams, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by DISCOVERY OmniMap anti-Rb HRP secondary antibody (Roche Tissue Diagnostics, ref. 05269679001) for 12 minutes ...
• Spatial resolution of transcriptomic plasticity states underpinning lethal morphologies in lung adenocarcinoma

  Hannah L Williams, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by DISCOVERY OmniMap anti-Rb HRP secondary antibody (Roche Tissue Diagnostics, ref. 05269679001) for 12 minutes ...
• Spatial resolution of transcriptomic plasticity states underpinning lethal morphologies in lung adenocarcinoma

  Hannah L Williams, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by DISCOVERY OmniMap anti-Rb HRP secondary antibody (Roche Tissue Diagnostics, ref. 05269679001) for 24 minutes ...
• Loss of asgr1a leads to the secretion of excess dietary cholesterol in zebrafish

  Tabea O.C. Moll, et al., bioRxiv - Pathology 2024

  Quote: ... generated sense and antisense digoxigenin-labeled riboprobes (Anti-Digoxigenin-AP, Fab fragments, Roche 11093274910). WISH was performed three times with an n > 5 on AB larvae stages 3 - 7 dpf and on 6 dpf larvae carrying either the asgr1ac830/c830 or the asgr1ac865/c865 mutation ...
• Multivalent Afadin Interaction Promotes IDR-Mediated Condensate Formation and Junctional Separation of Epithelial Cells

  Shuhei Kuno, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-HA rat mAb (#11867431001, clone 3F10, Roche; 1:4000 dilution for western blotting), anti-α-tubulin mouse mAb (#T6199 ...
• Single-molecule mechanical unfolding kinetics of unmodified Saccharomyces cerevisiae tRNA^Phe: a hint to the tRNA chaperone-tRNA interaction mechanism

  Wenzhao Liu, et al., bioRxiv - Biophysics 2021

  Quote: ... at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche, Basel, Switzerland) coated coverglass surface at the other end through digoxigenin-antibody interactions ...
• FLS2 is a CDK-like kinase that directly binds IFT70 and is required for proper ciliary disassembly in Chlamydomonas

  Qin Zhao, et al., bioRxiv - Cell Biology 2019

  Quote: ... The lysates were incubated with 30 μl pre-washed rat anti-HA Affinity Matrix (Roche) for 3 h at 4°C ...
• Shugoshin promotes efficient activation of spindle assembly checkpoint and timely spindle disassembly

  Aakanksha Sane, et al., bioRxiv - Cell Biology 2020

  Quote: ... was used for staining Cse4-GFP and rat anti-HA (1:200 dilution: Roche, 11867423001) for Mad2-3HA staining as primary antibodies ...
• Behaviour-driven Arc expression is greater in dorsal than ventral CA1 regardless of task or sex differences

  J. Quinn Lee, et al., bioRxiv - Neuroscience 2021

  Quote: ... DIG-labelled Arc riboprobe signal was amplified with anti-DIG-POD (1:300; Roche Diagnostics), Tyramide Signal Amplification (TSA ...
• Balanced release of neuropeptide FF and gonadotropin-releasing hormone 3 modulates male sexual behavior

  Chie Umatani, et al., bioRxiv - Neuroscience 2021

  Quote: ... and alkaline phosphatase-conjugated anti-DIG antibody (diluted 1:1,000 in DIG-1, Roche Diagnostics) for 2 h and washed twice with DIG-1 for 5 min each ...
• CBP/p300 orthologs CBP2 and CBP3 play distinct roles in planarian stem cell function

  Clara R. Stelman, et al., bioRxiv - Developmental Biology 2020

  Quote: ... probes were detected with anti-digoxigenin Fab fragments conjugated with alkaline-phosphatase (1:2000, [Roche]). 5-Bromo-4-chloro-3-indolyl phosphate (BCIP ...
• Single-cell analysis reveals cellular heterogeneity and molecular determinants of hypothalamic leptin-receptor cells

  N. Kakava-Georgiadou, et al., bioRxiv - Neuroscience 2022

  Quote: ... for 1 hr at RT and incubated with anti-DIG-POD (1:500, 11207733910, Roche) and rabbit anti-RFP (1:1000 ...
• The Mitochondrial RNA Granule Modulates Manganese-Dependent Cell Toxicity

  E. Werner, et al., bioRxiv - Cell Biology 2022

  Quote: ... pH 7.4 (Buffer A) with 0.5% Triton X-100 and Complete anti-protease (Roche, 11245200) was added to each plate ...
• Regulation of melanocyte development by ligand-dependent BMP signaling underlies oncogenic BMP signaling in melanoma

  Alec K. Gramann, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Hybridized probes were detected using anti-digoxigenin (DIG) antibodies tagged with alkaline-phosphatase (AP) (Roche) using NBT/BCIP (Roche ...
• Delta-1 functionalized hydrogel promotes hESC-cardiomyocyte graft proliferation and maintains heart function post-injury

  Kaytlyn A Gerbin, et al., bioRxiv - Bioengineering 2019

  Quote: ... followed by overnight incubation with a peroxidase-conjugated anti-BrdU primary antibody (1:40, Roche). Slides were then incubated with Alexa Fluor-488 goat anti-mouse (1:100 ...
• Regulation of nerve growth and patterning by cell surface protein disulphide isomerase

  Geoffrey M.W. Cook, et al., bioRxiv - Developmental Biology 2019

  Quote: ... This was replaced with blocking solution containing alkaline phosphatase-conjugated anti-digoxigenin Fab fragments (Roche) at 1:2000 dilution ...
• Active mode of excretion across digestive tissues predates the origin of excretory organs

  Carmen Andrikou, et al., bioRxiv - Evolutionary Biology 2019

  Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP ...
• Requirement of Irf6 and Esrp1/2 in frontonasal and palatal epithelium to regulate craniofacial and palate morphogenesis in mouse and zebrafish

  Shannon H. Carroll, et al., bioRxiv - Developmental Biology 2020

  Quote: ... WISH colorimetric signal detection was performed using an alkaline phosphatase-conjugated anti-DIG antibody (Roche) and BM Purple AP substrate (Roche).
• Transient Nodal signalling in left precursors coordinates opposed asymmetries shaping the heart loop

  Audrey Desgrange, et al., bioRxiv - Developmental Biology 2019

  Quote: ... Hybridization signals were detected by alkaline phosphatase (AP)-conjugated anti-DIG antibodies (1/2500; Roche), which were revealed with NBT/BCIP (magenta ...
• An ER CREC family protein regulates the egress proteolytic cascade in malaria parasites

  Manuel A. Fierro, et al., bioRxiv - Cell Biology 2019

  Quote: ... The antibodies used for IFA were: rat anti-HA antibody (clone 3F10; Roche, 1:100), mouse anti-AMA1 (1F9 from Alan Cowman) ...
• Cryo-EM structures reveal how ATP and DNA binding in MutS coordinate the sequential steps of DNA mismatch repair

  Alessandro Borsellini, et al., bioRxiv - Biochemistry 2021

  Quote: ... so that both DNA ends are blocked with 200 nM anti-digoxigenin Fab fragments (Roche Diagnostics ...