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Citations for Roche :
1851 - 1900 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in 4 times the pellet volume of sucrose buffer (10 mM HEPES, pH 7.5, 250 mM sucrose, 2 mM magnesium acetate, 1X cOmplete EDTA-free protease inhibitor cocktail [Roche]) and lysed by douncing at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and resuspended in solubilization buffer (50 mM HEPES/KOH pH 7.5, 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche] ...
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bioRxiv - Developmental Biology 2023Quote: ... RPE explants were collected in 60 μl of lysis buffer (Tris-HCl 62.5 mM, SDS 2%, pH 6.8) with protease (Roche, #1183617001) and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Transgenes were identified using PCR primers specific for each transgene (Suppl. Table 2) amplified using Taq DNA polymerase (Roche) and Failsafe Buffer D (Epicentre) ...
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bioRxiv - Molecular Biology 2023Quote: ... in complementary deoxyribonucleic acid (cDNA) from 10 ng total RNA in 10 μl reactions with 2× Master Mix (Roche) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2023Quote: Cell lysis was performed by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 0.5 mM DTT; 2 µM avidin, protease inhibitors cocktail (Roche) and DNAse (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... skin tissues were homogenized at a 10% (w/v) concentration in a lysis buffer containing 2 mM CaCl2 and 0.25% (w/v) collagenase A (Roche) in Tris-Buffered Saline (TBS) ...
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bioRxiv - Molecular Biology 2023Quote: Successful virus rescue was monitored with SARS-CoV-2 Rapid Antigen Test (Cat# 9901-NCOV-01G, SD BIOSENSOR, Roche) by applying 100 μL of unfiltered culture supernatant directly onto the test device.
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA using Superscript II reverse transcriptase (Roche, Mannheim, Germany). qPCR was performed in a 20 μl reaction mixture containing 10 μl of SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-labelled cDNA probe was incubated at 95 °C for 10 min and immediately placed in the ice for 5 min before adding to the hybridization buffer (5x SSC, 50 % formamide, 0.02 % SDS, 2 % blocking agent (Roche), DEPC-treated water) ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl containing a ~2-fold molar excess of FimC and complete EDTA-free protease inhibitor cocktail (Roche). The solution was immediately desalted and the buffer exchanged by passing it over a Sephadex G25 (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... disulfide-bonded FimI at 4 °C 30-fold into 20 mM acetic acid-NaOH pH 5.0 containing a ~2-fold molar excess of FimC and complete EDTA-free protease inhibitor cocktail (Roche). FimCI was then dialyzed against 20 mM MOPS-NaOH pH 6.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were lysed in lysis buffer (2% CHAPS, 50 mM HEPES, pH 7.4, 150 mM NaCl, 1mM Na3VO4, 1X EDTA-free PIC (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellet was dissolved in lysis buffer 2 (1x HEMG with 200 mM NaCl, 0.5% NP-40, 1x protease inhibitor cocktail (Roche), 0.5mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were washed with 2 X SSC and 0.2 X SSC for 20 min followed by incubation in a blocking solution containing 2% blocking reagent (Roche) and 20% sheep serum for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were harvested by cell scraping into 2 ml of PBS with a protease inhibitor cocktail (Roche cOmplete 4693159001). The cell suspension was centrifuge at 200xg for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 20 mM Tris-HCl (pH 7.4) with 2% SDS supplemented with a proteinase inhibitor (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Roche). Protein concentration of lysates was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2022Quote: ... Pellets were further extracted by sonication in 3x volumes of SDS lysis buffer (50□mM Tris-HCl, pH 7.4, 2% SDS, 1X Complete Mini protease inhibitor cocktail [Roche]) with centrifugation at 21,000 x g for 30 min at 25°C to obtain the SDS-soluble fraction (Triton-insoluble) ...
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bioRxiv - Biochemistry 2023Quote: ... Ndel1) were diluted in assay buffer at concentrations indicated in Figure legends supplemented with 2 mM AMP-PNP (Roche). Prior to image acquisition ...
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bioRxiv - Immunology 2023Quote: ... lysed by ice-cold RIPA buffer (50 mM Tris pH8, 150 mM NaCl, 2 mM EDTA, 0.5% NP40) supplemented with protease inhibitors (Roche) and by sonication ...
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bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2% (w/v) SDS and immediately prior to use supplemented with cOmplete™protease inhibitor cocktail (Roche. Cat# 04693116001) and PhosStop (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... whole-exome DNA from blood or salivary samples was captured using the Nimblegen v.2 exome capture reagent (Roche) or Nimblegen SeqxCap EZ MedExome Target Enrichment Kit (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 μg of total RNA was retro-transcribed using Transcriptor First Strand cDNA Synthesis Kit (Roche; Cat #04897030001) following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: Fibroblast proliferation was assessed using a 5-bromo-2’-deoxyuridine (BrdU) incorporation assay (colorimetric) from Roche (Indianapolis, IN, USA) for 48 h following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The total lysate was clarified by centrifugation at 85,000×g for 30 minutes and then mixed with 2 mL cOmplete His-tag NiNTA beads (Roche) pre-equilibrated in purification buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were analyzed for glucose production rate at 2 h by using an Accu-Chek® Aviva glucometer (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... embryos were with PBT (x3, 15mins) and incubated in prehybridization buffer (50% deionized formamide, 5× SSC, pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... The ganglia were minced with iridectomy scissors into fine pieces and then transferred to a 35 mm petri dish containing 2 ml enzyme mix (0.4 mg/ml Trypsin (Roche), 1.2 mg/ml collagenase D (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... in nuclei resuspension buffer of 1x PBS with 2% BSA and 0.2 U/µl Protector RNase Inhibitor (Roche, USA). Resuspended nuclei were then overlaid on 750 μl of 29% Optiprep in nuclei resuspension buffer before centrifuging at 13,000 g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and reversely transfected with 0.2 µg HiBIT –tagged SETDB1 (3TD) and 1.8 µg of empty plasmid using X-tremeGene XP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... cells were re-suspended and lysed in ice-cold RIPA buffer (50 mM Tris pH8, 150 mM NaCl, 2 mM EDTA, 0.5% NP40) with protease inhibitor cocktail (Roche) and sonicated ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)) supplemented with cOmplete™ EDTA-free Protease Inhibitor Cocktail Tablets (Roche) and lysed by sonication ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were resuspended in lysis buffer (25 mM HEPES pH 7.4, 2 mM EGTA) supplemented with EDTA-free cOmplete protease inhibitor cocktail (Roche). Cells were lysed by five passages through a chilled Emulsiflex C3 homogenizer (Avestin) ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequent incubation with 80 units/mL collagenase II and 2 units/mL dispase II (catalog number: 04942078001, Roche) solution ...
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bioRxiv - Cancer Biology 2024Quote: ... or SDS extraction buffer (2% SDS, 50 mM Tris pH 6.8) with freshly added Complete protease Inhibitor Cocktail (Roche). Extracts were run on 10% polyacrylamide SDS-PAGE gels ...
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bioRxiv - Cell Biology 2024Quote: ... 22μl H2O) before the addition of 25μl amplification mix 2 (0.75μl Expand high fidelity enzyme mix (Roche, cat. 11732641001), 5μl 10X Expand high fidelity buffer with 15mM MgCl2 (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were thawed and resuspended in 10 µL benzonase buffer (50 mM Tris-HCl pH 8.0, 2 mM MgCl2) with EDTA-free protease inhibitor (Roche, 11873580001 ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Plant Biology 2021Quote: ... PMSF 1 mM and 1 protease inhibitor tablet (Roche cOmplete EDTA free cat# 05056489001)/50 ml) ...
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bioRxiv - Microbiology 2020Quote: ... 1% Triton X-100) containing 1 tablet/50 ml of protease inhibitor cocktail (Roche), transferred to a tube and incubated for 15 min at 4°C on a wheel ...
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bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x protease inhibitor cocktail (cOmplete ULTRA Tablets, Mini, EDTA-free, Roche; 1:10), pH 8.5 ...
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bioRxiv - Microbiology 2021Quote: ... incubating in 1% blocking solution with 1:10,000 α-Digoxigenin-AP Fab fragments (Roche) for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-GFP (Cat no. 11814460001, 1:300 PLA, 1:4000 WB, Roche Diagnostics), mouse anti-Tubulin (Cat no ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg/ml-1 DNase I and 1 protease inhibitors (cOmplete EDTA-free, Roche) for each gram of pellet ...