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Citations for Roche :
1751 - 1800 of 3398 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Genetics 2020Quote: ... Proteins were separated by SDS-PAGE on 4-12% Bis-TRIS SDS-polyacrylamide gels (Novex, NP0322BOX) and analysis was done against primary antibodies α-HA (1:1000, Roche, 12CA5) or α-Tub2 (1:4500 ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexa-conjugated ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections were incubated with anti-HA antibody (1:400 dilution) followed by incubation with anti-rabbit-HQ (Roche; Cat#. 760-4815) and anti-HQ HRP (Roche ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexaconjugated ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... to prepare for an hour long blocking in 10% lamb serum/TBST and an overnight incubation at 4°C in a 1% lamb serum containing Anti-Dig-AP antibody (Roche, 1:5000). The next morning ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Probes were detected by incubation overnight at 4°C in a 1:5000 dilution of Anti-DIG-AP antibody (Roche, Catalog# 11093274910) in Blocking Buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... then treated with blocking reagent (Genostaff) for 15 min and alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were stained with secondary antibodies (1:300) for 2 h at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). Cells were mounted with ProLong Diamond Antifade Mountant (Thermo P36970 ...
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bioRxiv - Neuroscience 2024Quote: ... specific digoxigenin (DIG)-labeled RNA probes and performed nitro blue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate color-reaction (BCIP) after wash and incubation with anti-digoxigenin antibody (catalog. no. 11093274910; Roche; RRID: AB_514497) as previously reported (Zempo et al. ...
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bioRxiv - Pathology 2024Quote: ... the aortic sections were incubated overnight at 4°C in a water-humidified chamber with anti-DIG-AP antibody diluted in blocking buffer (Roche, 1:500), followed by a three-time wash with TBST on the next day ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were blocked in 5% (w/v) nonfat dry milk and probed with anti-HA antibodies (Roche 11867431001; 1:10,000 dilution), followed by horseradish peroxidase-coupled secondary anti-rat antibody (Sigma-Aldrich A9037 ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (Cytiva, clone BU-1) and rat anti-HA (Roche, clone 3F10) antibodies diluted 1:500 in block ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described in previous work (58) and detected with anti-digoxigenin-POD or anti-fluorescein-POD antibodies (1:2000, Roche/Sigma-Aldrich) blocked with 10% heat-inactivated Horse serum and 10% Western Blotting Blocking Reagent (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were the same as above and OmniMap anti-Ms HRP and anti-Rb HRP (Roche Cat#5269652001 and Cat#5269679001) secondary antibodies were coupled to OPAL520 (AKOYA Biosciences Cat# FP1487001KT) ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in 5% milk in PBS with 0.001% Tween-20 (PBST) before incubation with the specified antibodies prepared in 5% milk (GFP (Roche, 11814460001, 1:1,000), FLAG-HRP (Merck ...
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bioRxiv - Biophysics 2021Quote: ... Cells were unroofed using distilled water containing complete™ protease inhibitors (Roche) and 10 μg/mL phalloidin (Sigma-Aldrich P2141 ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: Total RNA was isolated from cells with High Pure Isolation Kit (Roche). For cDNA synthesis random nonamers ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed with LBX supplemented with EDTA-free protease inhibitors (Roche), phosphatase inhibitors (Halt phosphatase ...
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bioRxiv - Developmental Biology 2021Quote: ... Apoptosis was detected with the In Situ Cell Death Detection Kit (Roche) according to the product manual ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL was performed using an In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then treated with 25 μg/mL RNase A (10109142001, Roche) in PBS 1× at 37 °C for 20min ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were monitored every 10 minutes by the xCELLigence system (Roche) for proliferation ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in lysis buffer supplemented with Complete protease inhibitor (Roche) and lysed with a microfluidizer (Microfluidics) ...
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bioRxiv - Cancer Biology 2020Quote: ... The fragments were further enzymatically dissociated into single cells using LiberaseTM (Roche) with a final concentration of 0.26 U/mL and a final volume of 25ml Liberase solution/0.5g tissue on a shaker with a speed of 85 rpm for 45 min at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... cells were grown in media containing 0.1 mg/mL Hygromycin (H3274, Roche).
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bioRxiv - Cancer Biology 2020Quote: Cell viability was evaluated using the colorimetric WST-1 assay (Roche Diagnostics). After a 4-h incubation period with WST-1 reagent the absorbance was measured at 450 nm/ 600 nm using a microplate reader (ELISA-Reader Zenyth 200st ...
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bioRxiv - Cell Biology 2021Quote: ... Both cells and tissues were blocked in 1x Western Blocking Reagent (Roche). The primary antibodies used in this study included ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293T cells were transfected with X-tremeGENE HP (Roche Diagnostics, Rotkreuz, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... anti-3xHA (1:2000, 12CA5, Roche or 1:4000, C29F4, Cell Signaling) anti-PfALD (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed in RIPA buffer supplemented with 1x Complete inhibitor (Roche) and 1U/μl Universal Nuclease (Pierce) ...
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bioRxiv - Biophysics 2020Quote: ... one flow cell volume of 100 μg/ml anti-digoxigenin (Roche, Switzerland) in 1x PBS is introduced ...
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bioRxiv - Cell Biology 2022Quote: ... TUNEL was performed using the In Situ Cell Death Detection Kit (Roche).
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bioRxiv - Microbiology 2020Quote: ... Hsu cells were transfected using X-tremeGENE HP DNA Transfection Reagent (Roche) with plasmid(s ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in RIPA buffer containing complete mini protease inhibitor (Roche) for 15 minutes on ice with occasional vortexing ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then blocked for 10 minutes with 0.5% blocking reagent (Roche) in 0.1 M Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with various plasmids in duplicate using XtremeGENE (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were transfected by XtremeGene HP as per the manufacturer’s specifications (Roche). When cells were about 80% confluent a total of 500 ng of plasmid DNA was transfected per well ...
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bioRxiv - Physiology 2021Quote: The in situ cell death-detection kit from Roche (TUNEL; Indianapolis, IN) assay was used to detect DNA fragmentation in embryos as described previously (Kalo and Roth ...