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Citations for Roche :
1701 - 1750 of 5641 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 5 μL of glycogen (20 mg/ ml) and 5 μl of proteinase K (20 mg/ ml; Roche) were added to the samples and incubated at 37 °C for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... Brain was mechanically and enzymatically digested in collagenase/dispase (1mg/mL) and DNase (10mg/mL; Roche Diagnostics). Lung tissue was mechanically and enzymatically digested with Collagenase/Hyaluronidase (3000U and 1000U ...
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bioRxiv - Cell Biology 2021Quote: ... and digested in Han s’ balanced salt solution containing 0.18 units/mL collagenase (10 mg/mL; Roche) for 45min at 37°C ...
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bioRxiv - Genetics 2019Quote: ... DMEM was aspirated and replaced with 20 mL DMEM containing 0.5 mg/mL Liberase TM (Roche, 5401127001) and incubated at 32°C for 15 min at 500 rpm ...
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bioRxiv - Immunology 2021Quote: ... composed of 1 mg/mL collagenase D and 0.1 mg/mL DNase I (both from Roche, Switzerland) in complete RPMI medium (Gibco ...
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bioRxiv - Physiology 2022Quote: Islets were isolated by injecting 2 mL collagenaseP (0.8 mg/mL in HBSS, Roche Diagnostics, Catalog # 11249002001) into the bile duct with the ampulla of Vater clamped ...
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bioRxiv - Physiology 2023Quote: ... Both kidneys were incubated in 10 ml enzymatic solution (1 mg/ml collagenase A (Roche Diagnostics, Germany), 2.5 mg/ml trypsin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... plus 2% fetal calf serum (Dutscher)) supplemented with 50μg/mL DNAse and 10μg/mL collagenase D (Roche).
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bioRxiv - Plant Biology 2019Quote: ... Adapter-ligated cDNA fragments were enriched and amplified with 15 cycles of PCR using the HiFi NGS Library Amplification kit from KAPA Biosystems. Libraries with were multiplexed at equimolar concentration and sequenced on the Illumina HiSeq 2500 for 1×40 bases ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was stopped by the addition of 500 ng of plasmid (size > 10 kb) and 0.1 pmol of ATP-γ-S tetralithium salt (10102342001-Roche, Sigma) followed by incubation for 30 min at 30°C ...
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bioRxiv - Immunology 2022Quote: ... The normalization of DNA amount was performed by quantifying albumin gene copies with qPCR using human genomic DNA (20 ng/µl) standard (Roche, #11691112001) as we previously described16.
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of RNA for each sample were reverse transcribed using the Transcriptor first strand cDNA synthesis kit (Roche, Germany). For real-time quantitative PCR reactions ...
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bioRxiv - Bioengineering 2020Quote: ... For each triplicate cDNA amounts corresponding to 50 ng RNA were used and measurements were conducted in a LightCycler® 480 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480; Roche Diagnostics, Mannheim, Germany) with SYBR Green I dye and gene-specific primers (Supplemental Table S8) ...
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bioRxiv - Neuroscience 2021Quote: ... and RNA-Seq libraries were prepared from 500 ng total RNA using the KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems) for rRNA depletion ...
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bioRxiv - Genomics 2020Quote: Whole genome sequencing libraries were generated from 1000 ng of genomic DNA spiked with 0.1% (w/w) unmethylated λ DNA (Roche Diagnostics) and fragmented to 300–400 bp peak sizes using the Covaris focused-ultrasonicator E210 ...
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bioRxiv - Cancer Biology 2022Quote: NGS libraries were prepared from extracted gDNAs following a 2-step PCR protocol with 2 x KAPA Mastermix (KK2612, KAPA Biosystems). For spleen Tregs and CD4s ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 600 ng of purified gDNA was used to construct a sequencing library using KAPA HyperPlus library preparation kit (Roche Diagnostics). Final library QC for size distribution verification was done on Caliper Lab Chip GX (Perkin Elmer ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries were quantified using a Tapestation DNA 1000 Screen Tape and by qPCR using an NGS Library Quantification Kit (KAPA Biosystems) on an AriaMx qPCR system (Agilent ...
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bioRxiv - Genomics 2019Quote: ... a total of 12 µg of DNA was split into 24 50 µL PCR reactions (each with 500 ng of input DNA) with KAPA2G Robust HostStart ReadyMix (Kapa Biosystems) for three cycles with a 65 °C annealing and 40 s extension ...
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bioRxiv - Biophysics 2019Quote: An automated dual-indexed library was constructed with 250 ng of genomic DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng of cDNA were used for qPCR with the KAPA SYBR FAST qPCR Kit (KK4604, KAPA Biosystems, Wilmington, MA, USA) and the experiment run on the Biorad CFX96 system using the TqPCR protocol described in Zhang et al ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed on 300 ng RNA prior to Reverse Transcription Polymerase Chain Reaction (RT-PCR) using RNAse-free DNAse I (Roche, # 04716728001) at 37°C for 10 min ...
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bioRxiv - Genomics 2020Quote: ... Samples were hybridized with digoxygenin-labelled oligonucleotide probes directed to α-globin introns (30 ng per slide) and visualized using FITC-conjugated antibodies (primary: sheep anti-DIG FITC [Roche] 1:50 ...
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bioRxiv - Genomics 2020Quote: ... The six STARR-Seq RNA replicate libraries and a STARR-Seq Plasmid DNA input library (amplified as before but with 20 ng DNA input and 15 PCR cycles) were normalized with the KAPA Library Kit (KAPA Biosystems) and sequenced on an Illumina NextSeq with 150-bp paired-end reads using standard protocols.
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bioRxiv - Genetics 2019Quote: ... NGS sequencing adapter addition and multiplex indexing of DNA samples was achieved using the KAPA Hyper Prep Kit (KAPA Biosystems KK8502) according to manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: ... Real-time PCR was performed in a final volume of 20 µl containing 20 ng of cDNA using SYBR Fast Universal qPCR Kit (Kapa Biosystems) and analyzed using the Quant Studio 6 Flex system (Applied Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... 250 ng of genomic DNA was sheared and libraries were prepared using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA). For each RNA sample ...
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bioRxiv - Genomics 2020Quote: ... Synthesis of cDNA was performed with 150 ng of total RNA using the First Strand cDNA Synthesis kit (Roche Applied Science) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... 250 ng of total RNA was used to prepare barcoded RNA-seq libraries using Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems). Samples were sequenced on the HiSeq 2500 (Figure 2 ...
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bioRxiv - Cell Biology 2022Quote: ... Bulk RNA-seq libraries were prepared from 500 ng of total RNA for each sample using KAPA mRNA HyperPrep Kit for Illumina (Roche, #KR1352) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina) (Kapa Biosystems), using 10 cycles of PCR amplification ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated chromatin was then indexed with the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences 21024) and amplified with KAPA Library Amplification Kit (Roche KK2620), before paired-end next generation sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Genomics 2023Quote: ... 900 ng from the breeding population) were used in a liquid-phase capture (SeqCap EZ developer, IRN 6089042357, OID35086, Roche Nimblegen). The captured material was amplified and sequenced on an Illumina HiSeq 4000 PE100 at the Centre d’Expertises et de Services Génome Québec (Montréal ...
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bioRxiv - Cell Biology 2023Quote: Library preparation was performed with 500 ng of total RNA where the ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems), fragmented in reverse transcriptase buffer and heated to 94°C for 8 minutes ...
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bioRxiv - Molecular Biology 2023Quote: Automated dual indexed FFPE libraries were constructed with 50-250 ng of genomic DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... All purified nucRNA (9-20 ng/sample) was used to construct cDNA sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase (KK8560, KAPA Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We ran indexing PCR on our NGS samples by creating the following mix per sample: 1x Kapa Hifi readymix HS (Roche, 7958935001), 0.5uM common primer (Table 1) ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared from 150 ng of rRNA-depleted RNA following the KAPA Stranded RNA-Seq with RiboErase workflow for Total RNA-Seq libraries (KAPA Biosystems). Library quality was evaluated using the Qubit® 3.0 Fluorometer and the Agilent 2100 Bioanalyzer instrument ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng of DNA from each sample was analyzed using the Kapa Cyber Fast Q-PCR Kit (Kapa Biosystems, Wilmington, MA). The following rDNA primers (designed using Primer3Plus software ...
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bioRxiv - Developmental Biology 2024Quote: ... Per sample 200 ng of total RNA was used for library preparations with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems), including steps of the oligonucleotide hybridization ...
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bioRxiv - Genomics 2024Quote: ... We used 10 ng of DNA from two biological replicates to generate sequencing libraries using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Adapted fragments were amplified by 12 cycles of PCR using the Kapa Hifi Hotstart NGS library Amplification kit (Roche, Basel, Switzerland), followed by 0.8x AMPure XP (Beckman Coulter Genomics ...
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bioRxiv - Cancer Biology 2023Quote: ... and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems) on a QuantStudio™ 6 Realtime PCR System (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with λPPase or that purified from Calyculin A-treated cells was subjected to proteolytic digestion with 0.01 ng/µl trypsin (Roche, sequencing grade) for 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-sequencing (RNA-seq) libraries were prepared using 750 ng of total RNA using the KAPA stranded mRNAseq kit (Roche, 8098123702) according to the manufacturer’s specifications ...