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Citations for Roche :
1701 - 1750 of 2505 citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... IFNγ-R2-eGFP plasmid was transfected in HeLa or Caco-2 cells with X-tremeGENE HP DNA transfection reagent (Roche, 6366236001) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... brucei cells were washed twice with PBS and lysed in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EGTA, 0.2% NP-40, Roche Complete protease inhibitor) for 20 min at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 2 mM CaCl2) containing 0.3 M of sucrose and MNase (30 units of MNase for every 2 million sperm, Roche Nuclease S7) was added to each tube ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
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bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... HepG2s were washed once with 1X PBS then lysed in SDS lysis buffer (50 mM Tris HCl/2% SDS/5% glycerol/5 mM EDTA/1mM NaF/dH2O) supplemented with cOmplete Protease Inhibitor Cocktail Tablets (Roche #11836170001), Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich® #P5726) ...
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bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 2 μM MG-132 proteasome inhibitor (#S2619, SelleckChem) and cOmplete® ULTRA EDTA-free inhibitor cocktail (#5892953001, Roche). Approximately 0.1 ml of 0.6 mm glass beads were added and thoroughly mixed ...
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bioRxiv - Cell Biology 2022Quote: ... 200 mM NaCl; 2 mM MgAc; 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]) per 1 g of cell pellet and incubated for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
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bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche, 04379012001), and a 370-bp fragment (nucleotides 1304- 1673 of NM_010610.3 ...
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bioRxiv - Microbiology 2023Quote: ... The elution fractions were collected in tubes that were previously supplemented with 2 µl Protector RNase inhibitor (Roche Diagnostics, Karlsruhe, Germany). The elution fractions were split ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM NaCl, 2 mM MgCl2, 10 mM NaF, 1 mM PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 45 min on ice and centrifuged at 20000g for 10 min at 4°C ...
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bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (used only for CDKN1A ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in mHBSS at a density of 107 cells/ml and pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM in a BSA-coated centrifugation tube ...
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bioRxiv - Molecular Biology 2024Quote: Nuclei protein from MOLM-13-Cas9 cells transduced with either sgROSA or sgTOPORS-1 treated with 2 doses of DAC or vehicle over 24hrs was extracted in RIPA buffer supplemented with protease inhibitor (Roche, #04693159001). 10ug of protein was boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... BmE cells were transfected with 2 μg of this recombinant plasmid and A3 helper plasmid using X-tremeGENE HP DNA Transfection Reagent (Roche, Switzerland), and the culture medium was changed after 6 h ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfections were performed using FuGENE 6 (Roche Diagnostics) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected using FuGENE®6 (Roche) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... and 5 μg/mL glucose-6-phosphate dehydrogenase (Roche)) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... were co-transfected with pLentiCRISPRv2 containing the indicated sgRNA sequences or pCW57 with the indicated cDNAs with XTremeGene 9 Transfection Reagent (Roche). After 12 hours ...
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bioRxiv - Immunology 2021Quote: ... 1.0 × 106 THP-1 cells were transfected with mixture of two sgRNAs-expressing plasmids (0.5 µg each) using X-tremeGENE™ 9 DNA Transfection Reagent (6365779001, Roche) according to manufacturer’s manual ...
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bioRxiv - Cell Biology 2019Quote: ... allowed to adhere overnight and transfected with the indicated splicing reporter constructs (1 µg/well) using X-tremeGENE 9 (Roche). As shown in the Key Resources Table ...
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bioRxiv - Biochemistry 2021Quote: ... Then qPCR was performed in triplicate using 4 μl sample in 8-9 μl reaction with FastStart Universal SYBR Green Master Mix (Roche) and primers flanking each MseI cassette (Table S3).
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bioRxiv - Molecular Biology 2021Quote: ... in 48-well plates and transfected at 50-60% confluence with a total of 100 ng Dual-pMIR-report plasmids using X-tremeGENE 9 DNA Transfection Reagent (Roche), followed by transfection with mimics (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... along with packaging plasmids including psPAX2 and pMD2.G (from Dr. Guang Hu in NIH) using the X-treme GENE 9 transfection reagent (Roche). Viral supernatant was collected and cellular debris was removed by syringe filtering (0.45 μm pore size ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced in HEK293T cells using helper plasmids (VSVG and psPAX2) with X-tremeGENE 9 DNA Transfection Reagent (Roche). Target cell lines were infected with the virus and 10 μg/ml polybrene ...
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bioRxiv - Immunology 2020Quote: ... cells were cotransfected with both the reporter gene and one of the different putative transcription factors using X-tremeGENE 9 (Roche) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... All viruses were packaged for 48 hours following transfection of HEK-293T cells using X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Microbiology 2020Quote: ... 70-80% confluent CHO-K1 cells were transfected in 35 mm dishes using X-TREMEGENE 9 Transfection Reagent (Roche, 6365787001). Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection of pTON-BIOPS-Flag-EGFP-FOXO1/FOXO3 and pCDNA-HA-E2F1 in HEK293T cells was performed using ExtremeGene 9 (Roche). Transfection of siRNA smartpools targeting EMI1 ...
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bioRxiv - Cell Biology 2020Quote: ... pENTR-spCAS9-T2A-EGFP-gRNAs vector were transfected into H9 hESCs using X-treme GENE 9 DNA Transfection Reagent (Roche). After 24h transfection ...
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bioRxiv - Biochemistry 2021Quote: ... sgRNA expressing vector along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK-293T cells using the XTremeGene 9 transfection reagent (Roche). Similarly ...
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bioRxiv - Biochemistry 2021Quote: ... and pPB-tetIRB-tetON-BSD plasmid bearing CLPTM1L-mEGFP or pPB-tetON-BSD-tightFF plasmid bearing CLPTM1L-FLAG-6His using X-tremeGENE 9 DNA transfection reagent (Roche), and incubated at 37 °C for 2 days ...
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bioRxiv - Molecular Biology 2022Quote: Mouse pancreata from adult mice (9-14 week old) were perfused with a solution containing 1 mg/mL collagenase-P (Roche) in Hank’s buffered salt solution (HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK 293 cells stably-expressing GFP-DFCP1 and RFP-ATG9 were generated by transfection of HEK 293 GFP-DFCP1 cell with RFP-ATG9 using X-tremeGENE 9 (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The probes for the upstream regions of the pks1 (oligonucleotides 9 and 10) and ku80 (oligonucleotides 24 and 25) deletion constructs were synthetised using PCR DIG DNA Labeling Mix (Roche) and Taq DNA Polymerase (NEB) ...
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bioRxiv - Physiology 2022Quote: ... The hairpin vectors were co-transfected with the lentivirus expression plasmid and packaging plasmid into actively growing HEK293FT using X-tremeGENE 9 DNA transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Pooled library (18 total – nine from DNA and 9 from total RNA – see below) concentration was determined using a KAPA library quantification kit (Roche). Libraries were sequenced on a Illumina NextSeq 500 instrument (2×150bp paired-end reads ...