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Citations for Roche :
1651 - 1700 of 6348 citations for HDM Fluorescent Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... and 2 cocktails of protease and phosphatase inhibitor (Halt™, Fisher Scientific; Phosstop™-phosphatase inhibitor tablets, Roche). The Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM DTT buffer to which was added 1 tablet of complete EDTA-free protease inhibitors cocktail (Roche). The cells were lysed by passage through an Avestin high pressure system at 45 psi ...
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bioRxiv - Genomics 2023Quote: ... Whole genome amplification was performed by addition of 20 μL 2× KAPA HiFi HotStart Uracil+ ReadyMix (Roche KK2801), 0.4 μL 100 μM Nextera P5 index primer and 0.4 μL 100 μM Nextera P7 index primer and incubation at 98°C for 45 s ...
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bioRxiv - Plant Biology 2024Quote: ... The oocytes were homogenized in 2 ml Lysis buffer (Tris-HCl pH 8.0 50 mM, ½ tablet PhosSTOP (Roche), 1x cOmpleteTM EDTA-free proteinase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 2-2.5 hours at room temperature with a rat anti-HA antibody (Roche, #11867423001) at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... 20% v/v glycerol, 2 mM MgCl2, 0.5 mM EDTA, 0.2% NP40, 1 mM DTT, 1x Complete Protease inhibitors, Roche) and the suspension tumbled at 4 °C for 45–75 min ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... 0.1% NP-40) containing 2 μM MG-132 and cOmpleteTM mini EDTA-free protease inhibitor cocktail (Roche, #04693159001) for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Peritoneal cavities were lavaged by injection of 2 mL of sterile PBS containing 1X protease inhibitors (cOmplete, Roche) or stable isotope labeling with amino acids in cell culture (SILAC ...
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bioRxiv - Bioengineering 2024Quote: ... the infected lungs were transferred to gentleMACSTM C tubes and homogenized on a gentleMACSTM tissue dissociator (“m_lung_01” program) using HEPES buffer (2 mg/ml collagenase D (Roche) and 80 U/mL DNAse I (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 2 mM EDTA, containing ‘Complete mix’ protease inhibitors from Roche) and passed 10 times through a 26-gauge needle ...
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bioRxiv - Genomics 2024Quote: ... Cells were re-suspended in digestion buffer (50 mM Tris-HCl pH7.6, 2 mM CaCl2, 0.2% NP40) containing protease inhibitors (complete EDTA-free, Roche) and warm-up to 37°C for 2min ...
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bioRxiv - Immunology 2024Quote: ... System performance was assessed by an Fc/2 standard obtained from a commercial mAb preparation (Herceptin; Roche Diagnostics).
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bioRxiv - Immunology 2024Quote: ... nuclei were resuspended in 1mL ST buffer (nuclear flow cytometry, RNAseq experiment 2) or PBS/1% BSA/0.2UμL Protector RNAse inhibitor (Roche) (RNAseq experiment 1) ...
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bioRxiv - Cell Biology 2020Quote: Cell proteins were extracted by Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Scientifi, 88804) or Complete™ Lysis-M EDTA-free kit (Roche, 04719964001) according to the manuals ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of beta-globin was performed by a commercially available human genomic DNA kit (The LightCycler Control Kit DNA, Roche Diagnostics, Basel, Switzerland)69.
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bioRxiv - Immunology 2023Quote: RNA-seq libraries and libraries containing both ATAC-seq and TaPE-seq were quantified by qPCR using the KAPA Library Quantification Kit (Complete kit, Universal) (F. Hoffmann-La Roche AG, Basel, Switzerland) on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories Inc ...
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were purified by using a High Pure PCR product purification kit (B A High Pure PCR Product Purification Kit (Roche Diagnostics, Mannheim, Germany), and purified products were sequenced directly using an ABI BigDye Terminator Cycle Sequencing Kit ver ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was used for NGS library preparation using either the KAPA Library Preparation kit or the KAPAHyperPlus kit (KAPA Biosystems, MA, USA). Genomic libraries were captured with the HyperPlus Capture Exome Kit or the xGen Exome Panel V2 (Integrated DNA Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA libraries were created from 150 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboErase kit (Roche Diagnostics, Vilvoorde, Belgium), according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... The pooled library was cleaned using MO BIO UltraClean® PCR Clean-Up Kit (MO BIO, Carlsbad, CA) and quantified using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing was carried out as detailed in the EMP protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... mixed-stage transgenic worms expressing CDE-1::GFP were resuspended in equal volumes of 2× lysis buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche®cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... 50 mM Tris/HCl pH 7.4/50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Immunoprecipitation buffer (150 mM NaCl, 10 mM Hepes, 2 mM EDTA, 1% Triton, 1.5 mM MgCl2, 10% glycerol, protease inhibitor from Roche) and centrifuged at 14,000 × g ...
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bioRxiv - Cell Biology 2019Quote: ... mechanically disintegrated and partially digested in a solution of collagenase and trypsin [2 mg/ml collagenase (Roche or Sigma), 2 mg/ml trypsin (Dutcher Dominique or Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 2% SDS and 50 mM DTT with the addition of anti-protease (cOmplete cocktail, Roche 11 873 588 001). Samples were boiled 5 min at 95°C before loading on polyacrylamide gels ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal (3F10) antibody directed against the HA epitope and monoclonal (B-2) antibody against GFP were purchased from Roche and Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Immunology 2021Quote: ... Culture media was supplemented with 500 U/mL human IL-2 (Tecin from Roche, kindly provided by the NIH) starting on culture day 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 mM EDTA, 15% glycerol, 2 mM MgCl2, 1 mM DTT, 1 mM PMSF, and protease inhibitor cocktail [Roche]). Following clarification by centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... apob-1 and apob-2 levels were detected using the Fast Start Essential Green DNA master mix (Roche 06924204001) on a Roche LightCycler 96 instrument ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked in 1xBM Blocking/PBST at RT for 2 hours and incubated with anti-DIG-POD (1:1,000, 11207733910, Roche) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... DRGs from E13.5 embryos were dissected and placed in droplets of 2 mg/ml collagen (Roche Diagnostic, catalog#11179179001) together with COS1 aggregates transfected with either secreted Myc-Sema3A or control PAY1-GFP ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA probes were incubated for 2 hours at 37 degrees with Dig RNA labeling mix (11277073910, Roche, Basel, Switzerland) and SP6 or T7 RNA polymerase (RPOLSP6-RO and RPOLT7-RO ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of PCR gene specific primer (10X conc) (Table S1) and 10 μl of master mix (Roche 04707516001) in 20 μl reaction ...
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bioRxiv - Genomics 2020Quote: ... the pellets were washed with ice-cold nuclei suspension buffer (1X PBS containing 2% BSA and 0.2 U/µl Protector RNase Inhibitor, ROCHE) and filtered through a 30μm cell strainer (Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... For this 1 ml of worm pellet was mixed with 1 ml of 2x lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 2% Triton, 0.5 tablet of protease inhibitor (Roche, 05892791001)) ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR reaction (20 μL) consisted of 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48 h and resuspended in lysis buffer (50 mM HEPES pH 7.2, 200 mM NaCl, 10% glycerol, 2 mM DTT, and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed using a microfluidizer ...
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bioRxiv - Developmental Biology 2019Quote: ... Trunks from ∼60 embryos were homogenised in 1ml solubilisation medium [2%w/v CHAPS in PBS made 1mM with sodium orthovanadate and 1 tablet cØmplete protease inhibitor cocktail (Roche) per 50ml of solution] on wet ice by shearing through a 20G then 26G needle ...
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bioRxiv - Genomics 2020Quote: ... Cell suspension was then supplemented with 1 mM Tris (2-carboxyethyl) phosphine (TCEP) and 1× complete inhibitor cocktail (Roche). Cells were lysed by sonication on ice and then centrifuged at 80,000 g at 4 °C for 30 min ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... coli cells containing the overexpressed RNF114 fusion protein were re-suspended in 80 mL of lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 2 Roche protease inhibitor tablets [without EDTA] ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol, Roche Complete Protease Inhibitors and PhosSTOP Protein Phosphatase Inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and protease inhibitor cocktail (Roche) on ice for 20 min ...
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bioRxiv - Genetics 2019Quote: ... Next embryos were washed in blocking solution (100mM maleic acid, 150 mM NaCl pH 7.5, 2% blocking reagent (Roche)) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Cancer Biology 2021Quote: Human tumor samples were collected on different days right after surgery and digested in Hank’s Balanced Salt Solution supplemented with 2 mg/mL collagenase A (Roche), 2.5 U/mL hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...