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Citations for Roche :
1651 - 1700 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 137.5 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM sodium fluoride, EDTA-free protease inhibitor cocktail [Roche]). 1 mM orthovanadate was added to the lysis buffer to prevent binding of substrates to the PTP1B trapping mutant ...
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bioRxiv - Cell Biology 2019Quote: ... and resuspended in Laemmli sample buffer (2% SDS, 5% β 50 mM Tris-Cl pH 6.8) supplemented with Protease Inhibitor Cocktail and PhosStop (Roche) for isolation of total cellular protein ...
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bioRxiv - Cell Biology 2019Quote: Zebrafish samples for Western blot analysis were deyolked and directly lysed in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 10 ul per embryo unless otherwise indicated ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC cells for Western blot analysis were lysed directly in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 500ul per T-25 culture flask.
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bioRxiv - Cell Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... Seminiferous tubules retained on the filter were collected and incubated at 32 °C for 25 min in GBSS containing 1.2 mg/mL of Collagenase Type I and 5 μg/mL DNase (Roche 11284932). Cell aggregates were sheared gently by 10 rounds of pipetting with a wide orifice plastic transfer pipet and filtered through the Cell strainer to remove cell clumps ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was divided into 10 μl reactions containing 5 μg of RNA and was treated for 30 mins with 10 units of DNAse I (#04716728001; Roche). Then DNAse I was inactivated by addition of 1 μl of 25 mM EDTA and incubation at 70°C for 10 min.
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC ...
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bioRxiv - Neuroscience 2021Quote: ... and overlaid on top of 29% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 29% Iodixanol, 1x protease inhibitor (Roche, 11873580001), 0.4 U/ul RNAse inhibitor (Takara ...
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bioRxiv - Developmental Biology 2021Quote: The fourth and third mammary glands were dissected from 5.5 to 6.5-wk-old female mice, minced with scissors and digested in Digestion medium (DMEM/F12, 2mg/ml Collagenase A (Roche), 5μg/ml insulin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression signals were visualized using nitroblue tetrazolium/5-bromo-4-chloro-3-indolylphosphate solutions using a standard method (Roche). Whole-mount and sectioned specimens of Ciona were observed using an SZX12 stereo microscope (Olympus ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by visualization with nitro blue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP) (Roche Diagnostics, Basel, Switzerland). Embryos were imaged using a Leica M205 FA epifluorescence microscope (Leica ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% Tween 20) for 5 min each and then incubated in the AP reaction buffer with NBT/BCIP substrate (Roche).
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bioRxiv - Developmental Biology 2020Quote: ... The embryos were subsequently incubated in the dark on a slow rocker in dilutions of Nitro-blue tetrazolium/5-bromo-4-chloro-3-inodyl phosphate (NBT/BCIP; Roche) in TBST ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed embryos were homogenized in 2-5 ml freshly prepared lysis buffer (50 mM Tris pH7.5; 150 mM NaCl; 5 mM EDTA; 0.5 % NP-40; 1.15 % Triton X-100; 1x Roche Complete protease inhibitors) with a Dounce Homogenizer on ice ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... The PCR reaction contained 5 μl of 2X Kappa Hifi Hotstart ReadyMix (Kappa Biosystems, Roche Sequencing and Life Science, USA), 2 μl of 0.2 mg/ml BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... then harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES-KOH pH 7.0, 500 mM NaCl, 5 mM β-mercaptoethanol, 1x Protease Inhibitor Cocktail (Roche)) and lysed by sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-CAA GTG CAA CAG TTT CTC ATT-3’/5’-TGT TTG ACT ACA CTC ACA CT-3’) using X-tremeGENE 9 DNA Transfection Reagent (Roche). 48 hours after transfection ...
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bioRxiv - Physiology 2019Quote: ... for 45 seconds at 6,000 rpm x 3 (5 minutes on ice in between intervals) using a Roche Magnalyser instrument (Roche, Germany) and homogenization tubes containing ceramic beads (MagNA Lyser Green Beads ...
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bioRxiv - Cell Biology 2019Quote: 5 dpf zebrafish larvae were killed on ice and eyes dissected in a solution of 5 mM NaCl with protease inhibitor cocktail tablets (Roche). Eyes were either snap frozen in liquid nitrogen and stored at -80°C or immediately lysed ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 2 mM DTT, 30 mM imidazole and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 1 mM TCEP ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48-72 h and resuspended in lysis buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 30 mM imidazole, 1 mM TCEP and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed by sonication ...
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of DIG-probes was made in staining buffer (in 10% polyvinyl alcohol) supplemented with nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate (BCIP) (Roche). In addition ...
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bioRxiv - Immunology 2021Quote: Stimulated cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche #11836170001), 1 mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche# 11836170001), 1 mM Na3VO4 ...
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bioRxiv - Neuroscience 2020Quote: ... A single qPCR reaction is made of 5 μL 2xSYBR green mix (LightCycler® 480 SYBR Green I master, Roche), 2 μL H2O ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using 2-5 times the volume of RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Roche), PMSF (1 mM ...
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bioRxiv - Neuroscience 2019Quote: ... and hybridized overnight at 55°C with 1 ng/ml digoxigenin-labelled (GGGGCC)5 oligonucleotide probe (Integrated DNA Technologies) in 25 ml hybridization reaction (EasyHyb Granules, Roche). Following denaturation of the probe at 95°C for 5 min ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting cDNA was used as a template for qRT-PCR using 5× HOT FIREPol qPCR Mix (Solis BioDyne) on the LightCycler 96 System (Roche). Relative expression values were calculated with the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in a 10-μl volume containing 5 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of the corresponding primer pair (1.5 μM each) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantification of transcript levels was carried out using SYBR Green reactions with 5 ng cDNA in a 20 μl volume on a Light Cycler 480 II (Roche) relative to the reference gene UBC10 (POLYUBIQUITIN10 ...
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bioRxiv - Systems Biology 2020Quote: ... The filters were washed with 130 μl 50 mM ammonium bicarbonate and centrifuged again before overnight digestion with 100 μl 5 ng/μl trypsin (Roche) at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... and per sample eight PCR reactions with 4,000 beads each in 50µl using 1µM SMART PCR primer (oligos in supplementary table 5) and the 2x Kapa HiFi Hotstart Ready mix (Roche #07958935001) were performed ...
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bioRxiv - Microbiology 2021Quote: ... the remaining dermis was washed in Ca2+ and Mg2+ free PBS 5 times and incubated in a digestion buffer containing 2 mg/ml collagenase A (Roche), 100 µg/ml of DNase I (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
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bioRxiv - Genomics 2020Quote: ... 60 μl of anti-mouse magnetic beads were washed PBS/BSA (5 mg/ml BSA) and incubated with 3 μl of anti-Myc (Roche) or Anti-Rpb3 antibodies (Neoclone ...
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bioRxiv - Immunology 2021Quote: ... Lungs were cut into small pieces and digested in 5 mL of RPMI containing DNase I (100 μg/mL, Roche), Dispase II (800 μg /mL ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested by centrifugation at 3,800 × g for 10 min at 4 °C and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole, 5% glycerol and Complete Protease Inhibitor Cocktail Tablet (Roche), pH 8.0) ...
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bioRxiv - Physiology 2020Quote: ... RBC lysis and post-lysis wash buffers contained 5 mM Tris-HCl/pH 8.0 and complete Protease Inhibitor Cocktail tablets (cPIC; Roche, 04693116001), as opposed to sodium-phosphate buffer/pH 7.5 and PMSF and pepstatin A—a modification required to obtain good mass-spectrometry data ...
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bioRxiv - Neuroscience 2019Quote: The brain samples were homogenised in 5 volumes (w/v) of sterile PBS containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail tablets (Roche), sodium fluoride (10 mM ...
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bioRxiv - Plant Biology 2021Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Microbiology 2020Quote: ... Membranes was incubated secondary antibody for an hour in 5% milk and developed using Lumi-light western blot substrate (Roche) to detect HRP ...