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Citations for Roche :
1601 - 1650 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: All A673/TR/shEF in vitro and xenograft proteins were extracted with RIPA and anti-protease cocktail (Roche). Western blots were hybridized with rabbit monoclonal anti-FLI1 antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The TM protein was produced by transient transfection of HEK 293T cells with XtremeGene HP Transfection reagent (Roche) according to the manufacturer’s instructions and purified following a published protocol (48) ...
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bioRxiv - Immunology 2019Quote: ... These cells were then lysed in T-PER tissue protein extraction reagent (Pierce) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1(Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: After being pre-cleared with protein A/G-coupled Sepharose beads (Cat. 11134515001 and 11243233001, Roche, Mannheim, Germany) for 2 hrs ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (GE Water & Process Technologies) and treated with Western blocking reagent (Roche) for overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The protein was produced by transient transfection of HEK 293T cells with X-tremeGENE HP Transfection Reagent (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibodies were used to detect green fluorescent protein (GFP) (Cat#11814460001, clones 7.1 and 13.1, Roche). Rabbit polyclonal anti-Cdc42p antibodies were provided by Dr ...
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bioRxiv - Immunology 2022Quote: Protein extraction was performed using RIPA buffer in the presence of complete Mini EDTA-free protease inhibitor (Roche) and PhosSTOPTM phosphatase inhibitor (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Equal quantities of protein were electrophoresed on 10% SDS-PAGE and transferred onto a nitrocellulose membrane (Roche Diagnostics). The membranes were incubated with the primary antibody overnight at 4°C after blocking with 5% milk dissolved in TBST buffer for another 2 h at room temperature (25°C) ...
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bioRxiv - Molecular Biology 2022Quote: ... the frozen brain samples were homogenized in tissue protein extraction reagent (Pierce) supplemented with complete protease inhibitors (Roche), and centrifuged for 1 hour at 430 000 g at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The enriched FLC-Venus protein was detected by western blot assay with the antibody anti-GFP (11814460001, Roche). Signals were visualized with chemiluminescence (34095 ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted in RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: For immunoblot analysis of proteins, tissues were homogenized in RIPA buffer (R0278, Thermo) with proteinase inhibitors (11836170001, Roche) and phosphatase inhibitors (04906837001 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were pre-cleared by incubating with 30 μl of protein G-agarose beads suspension (Roche, cat. # 11719416001) for 3 h at 4°C on a mini-rotator ...
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bioRxiv - Molecular Biology 2023Quote: ... Two days after transfection cells were washed twice with ice-cold PBS and scraped in ice-cold Co-IP buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, protein inhibitor cocktail (Roche)) supplemented with 0.5% Nonident P40 ...
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bioRxiv - Cancer Biology 2023Quote: ... The His-tagged-TRF2TRFH protein was purified in presence of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) by using Protino ® Ni-TED 2000 Packed Columns (Macherey-Nagel ...
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bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... mitotic cells were obtained by mitotic shake-off and swelled in hypotonic buffer (75 mM KCl:0.8% NaCitrate:H2O at 1:1:1) with protease inhibitor cocktail (Roche) at room temperature for 10-15 min ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were generated by lysing cells in RIPA buffer (50 mM Tris-Cl [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1× Roche complete mini-protease inhibitor cocktail ...
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bioRxiv - Microbiology 2023Quote: ... pH 6.8, 10 mM DTT, 1 mM EDTA, 0.1% Tween, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche). The samples were centrifuged at 3000g for 6 min at 4°C using a tabletop centrifuge ...
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bioRxiv - Genomics 2024Quote: ... 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, 1× Protease Inhibitor cocktail, Roche) and incubated at 4 °C for 1 hour with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... targeting the HBV_circ_1 B site was labelled with DIG-11-dUTP by digoxin using a Dig high primer DNA labelling kit and detection starter kit II (Roche). Hepatic tissue paraffin sections of HCC patients were treated with dewaxing and then digested with protease K ...
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bioRxiv - Neuroscience 2021Quote: ... quantified using High Sensitivity DNA Kit run on Agilent 2100 Bioanalyzer and also KAPA Illumina library quantification kit (Roche, 7960140001) run on LightCycler 480 ...
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bioRxiv - Developmental Biology 2021Quote: Final RNA-seq libraries were quantified using the KAPA Library Quantification Kits for Illumina sequencing platforms (Roche, Kit code KK4824). 100bp paired-end sequencing reads (Illumina HiSeq2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from each sample using RNeasy Mini Kit (QUIAGEN) and cDNA was synthesized using Transcriptor First Strand cDNA Synthesis Kit (Roche). qPCR was performed in SYBR Green I Master Mix with sequence specific primers for genes of interest (Table 1. ...
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bioRxiv - Neuroscience 2021Quote: ... was used to capture lncRNAs in accordance with the SeqCap EZ Hybridization and Wash Kit and SeqCap EZ Accessory Kit (Roche). Captured lncRNA libraries were sequenced on an Illumina HiSeq 4000 platform with a read length of 150 bp x 2.
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA was amplified using ResolveDNA Whole Genome Amplification Kit (BioSkryb Genomics) and then libraries were prepared for sequencing (Kapa Hyperplus Kit, Roche). All libraries were sequenced on either an Illumina MiniSeq or NextSeq instrument (Illumina ...
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bioRxiv - Pathology 2022Quote: Total RNA was purified from cells using a Tiangen Easy Fast RNA Extraction kit and reverse-transcribed using Transcriptor First Strand cDNA Synthesis kit (Roche). Expression of mtDNA encoded genes were determined by quantitative real-time RT-PCR using SYBR Green PCR Master Mix (Roche Applied Science) ...
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bioRxiv - Neuroscience 2020Quote: Tissue samples were homogenized and RNA was extracted using an RNAeasy Mini Kit (Quiagen, Hilden, Germany) and reverse transcribed using a Transcriptor First-Strand cDNA Synthesis kit (Roche Diagnostics ...
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bioRxiv - Synthetic Biology 2019Quote: ... purified by gel electrophoresis and extracted from the gel via a commercial kit (HighPure PCR Product Purification Kit, Roche, DE). Sample preparation (Illumina TruSeq amplicon library ...
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bioRxiv - Plant Biology 2019Quote: ... The blots were hybridized with probes that were labeled using PCR DIG Probe Synthesis Kit and hybridization was detected with DIG Luminescent Detection Kit following the manufacturer’s instructions (Roche, Switzerland).
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bioRxiv - Genomics 2021Quote: ... Both libraries were quality controlled using a Fragment analyzer NGS kit (AATI) and qPCR with the Kapa library quantification kit (Roche) prior to sequencing.
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bioRxiv - Genomics 2022Quote: ... whole transcriptome mRNA-enriched RNA-Seq libraries were constructed from cell lysates using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA with the MagAttract HMW DNA extraction kit and prepared short-inserted paired-end libraries for the whole genome with the KAPA HyperPrep kit (Roche) PCR-free protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ug of total RNA was used for the construction of ribosome- depleted sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase kit (HMR, Roche) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments) and Chemiluminescent detection with CSPD following the instructions of the kit (all products and kits from ROCHE).
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bioRxiv - Plant Biology 2023Quote: ... The resulting library was assessed with Fragment Analyzer (Advanced Analytical Technologies) using the Genomic DNA Kit DNF-467 and quantified with KAPA Library Quantification Kit (Roche). A single lane of HiSeq3000 was used for sequencing with 2×151 bp read length in presence of 1% PhiX (Illumina) ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... the amount of LDH released during the in vitro experiments were quantified using the kit Cytotoxicity Detection Kit (LDH) provided by Roche. Triton X-100 (Applichem ...
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bioRxiv - Cancer Biology 2024Quote: ... and DAB staining was detected using the UltraView Universal DAB Detection Kit or the OptiView DAB IHC Detection Kit (all from Roche). The following antibodies were used ...
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bioRxiv - Zoology 2021Quote: ... quantified by qPCR Kapa Library quantification kit (Roche) and sequenced on an Illumina MiSeq (PE300 ...
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bioRxiv - Cell Biology 2020Quote: ... Signal was detected using LumiLight Plus Kit (Roche) on the Chemidoc MP imaging system (Biorad).
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bioRxiv - Genetics 2021Quote: ... and a High Pure Plasmid Isolation Kit (Roche), respectively.
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bioRxiv - Microbiology 2021Quote: ... quantified using a Kapa qPCR Library Kit (Roche), and sequenced using a MiSeq 500 v2 nano run (MiSeq Reagent Kit v2 ...
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bioRxiv - Microbiology 2019Quote: ... The Light Cycler 480 probe master kit (Roche) was used to amplify RSV cDNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the KAPA Library Quantification Kit (Kapa Biosystems). Data from sperm cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... quantified using the KAPA library quantification kit (Roche) and sequenced on an Illumina HiSeq 4000 with 2 × 150 bp paired-end runs.
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bioRxiv - Immunology 2020Quote: ... KAPA Hyper Prep Kit for Illumina (KAPA Biosystems), and NEXTflex DNA barcodes (Bioo Scientific) ...