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Citations for Roche :
1551 - 1600 of 4693 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Triton X-100) containing a protease inhibitor cocktail (#11836145001, Roche, Basel, Switzerland) and phosphatase inhibitors (04906837001 ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used were: HA clone 3F10 (11867423001, Roche; 1:100 dilution), NEUN (ABN91 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% Triton X-100 (w/v) supplemented with complete protease inhibitors (Roche). Lysates were incubated on ice for 15 minutes and vortexed at 5-minute intervals ...
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bioRxiv - Developmental Biology 2023Quote: ... and then exposed to mouse anti-digoxigenin antibody (Roche, #11333062910; 1/100 dilution) or rabbit anti-fluorescein antibody (Abcam ...
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bioRxiv - Genetics 2023Quote: ... 1% Triton X-100) supplemented with a protease inhibitor cocktail (Roche, Basel, Switzerland) on ice for 20 min with occasional agitation ...
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bioRxiv - Immunology 2023Quote: ... 1 % Triton X-100) supplemented with cOmplete Mini EDTA-free protease inhibitors (Roche). Lysates were sonicated ...
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bioRxiv - Immunology 2023Quote: ... 1 % Triton X-100) supplemented with cOmplete Mini EDTA-free protease inhibitors (Roche). Lysates were then sonicated ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100 supplemented with PMSF and cOmplete protease inhibitor cocktail (Roche) by sonication ...
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bioRxiv - Cell Biology 2024Quote: ... 12.5 mM EDTA) containing 100 μl proteinase K (recombinant PCR grade solution, Roche) was added to each sample and digested at 65 °C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 % w/v Triton X-100 supplemented with protease inhibitor tablets (Roche), 1 mM sodium fluoride and 1 mM sodium vanadate) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 % w/v Triton X-100 supplemented with protease inhibitor tablets (Roche), 1 mM sodium fluoride ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then exposed to blocking buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Immunology 2024Quote: ... 1% Triton X-100) supplemented with cOmplete™ Protease Inhibitor Cocktail (#11836170, Roche). Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µL of cell proliferation reagent WST-1 (Roche, #11 644 807 001) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... prior to the addition of 100 μL BM Blue POD soluble Substrate (Roche). Reaction was stopped with 1 N HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... brought to a final volume of 10 ml with 10% polyvinylalcohol solution) supplemented with 4.5 µl/ml NBT (Roche) and 3.5 µl/ml BCIP (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysis buffer was supplemented either with 40 U/ml RNase inhibitor (Molox) or 50 U/ml Benzonase (Roche) to differentiate between RNA- and protein-dependent interactions ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
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bioRxiv - Cancer Biology 2020Quote: ... and pancreatic tumor bearing mice by mechanical separation of the tumor followed by digestion with an enzymatic cocktail consisting of 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I (all from Roche) in PBS at 37°C for 2-3h with rotation at 600rpm ...
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bioRxiv - Immunology 2021Quote: ... The pancreas was inflated via the common bile duct by adding ∼3 ml of 0.8mg/ml Collagenase P (Roche) and 10µg/ml Dnase I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Microbiology 2020Quote: ... pellets were re-suspended in 1.2 mL of Lysis Buffer (50 μg/ml lysozyme, 0.8% NP-40, 1 mM MgCl2, 1x protease inhibitors [Roche; cOmplete Protease Inhibitor Cocktail] in PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Peels were cut in 2-5 mm2 pieces and placed in 5 ml digestion solution (0.75 mg/ml Liberase TH Research grade (Roche), 0.1 mg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple locations with a total of 1.5 mL RPMI containing 50 µg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP; Roche Diagnostics) for 27—42 h.
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... infused with digestion buffer (RPMI 1640 [Sigma], Liberase TL [0.1 mg/mL; Roche], and DNase [0.1 mg/mL; Roche]) and minced with scissors ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor tissues were manually minced and digested in 25 mL of Dulbecco’s Modified Eagle Medium (DMEM) containing 2 mg/mL of collagenase A (Roche) and DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 20 ml Tris- HCl (20 mM, pH7.5) supplemented with 10ug/mL DNase I (Roche, 10104159001) and ½ tablet cOmplete Protease Inhibitor Cocktail (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were dissociated in 1 μg/ml collagenase A plus 2.4 U/ml dispase II (enzymes, Roche Applied Sciences) in HEPES-buffered saline (HBSS ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Cell Biology 2020Quote: ... were incubating over night at 4°C with 0,375 mg/ml of NBT and 0,188 mg/ml BCIP dissolved in a solution of TMNbuffer (respectively #11383213001 and #11383221001, Roche).
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple sites with a total volume of 1.5 mL Liberase enzyme digestion solution (50 μg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma ...
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bioRxiv - Immunology 2020Quote: ... minced with scissors and digested at 37°C for 20-30 minutes in digestion buffer (0.52 U/ml Liberase TM [Roche] and 60 U/ml DNase I [Roche] in RPMI 1640 [Cellgro] with 5% fetal bovine serum) ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were transferred to a 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to 3-4 ml of digestion media containing 0.1 mg/ml Liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were dissociated in 1-2 ml of digestion mix (D-PBS, 250ug/ml Liberase TL (Roche; cat: 5401020001), 0.1 mg/ml DNaseI (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... washed three times 15 min each in PBST and pre-incubated for 1h at 60°C in Hybridization Buffer (HB: 50% Formamide, 2X SSC, 1mg/ml Torula RNA, 0.05mg/ml, Heparin, 2% Roche blocking reagent ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin, 1mM sodium pyruvate, 0.1% β-mercaptoethanol, 25mM HEPES, 150μg/ml DNase I [Sigma-Aldrich], 0.5mg/ml Liberase [Roche] and then slow spun at 50xg for 5 mins (11) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ml NP-40 in a total volume of 500 ml ddH2O) containing phosphatase and protease inhibitor cocktail (Roche) then centrifuged for 30 min at 12000 rpm at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and the lamina propria was minced and digested in RPMI medium containing 2.5 mg/ml Liberase TL and 20 U/ml DNase I (both from Roche) at 37°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The bacterial pellet was resuspended in lysis buffer (7.5 mL PBS supplemented with 1% Triton, 10 μg/mL DNaseI, EDTA-free Protease Inhibitor Cocktail (Roche)) and was incubated for 1 h on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... The probe was mixed in hybridization buffer (0.1 g/ml dextran sulfate [Sigma D8906-50G], 1 mg/ml Escherichia coli tRNA [ROCHE 10109541001] ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected cortexes were collected in 24 well-plate and cut into small pieces to be digested in 1.5 ml of digestion buffer containing 1 mg/ml of collagenase/dispase cocktail (Roche) and 0.5 mg/ml of DNase I ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was digested for 45-60’ at 37°C in a Thermomixer at 800 rpm in 1 mL of 1× PBS + 0.5 mM CaCl2 + 250 μg/mL Liberase (Roche) + 2 mg/mL of Collagenase (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Microbiology 2023Quote: ... washed one time with 1 ml phosphate-buffered saline (PBS) and resuspended in 0.3 ml PBS supplemented with complete mini protease Inhibitor Cocktail (Roche). Cell suspensions were transferred to lysis tubes containing glass beads and subjected to mechanical disruption in a homogenizer SpeedMill Plus (Bioanalytik Jena ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT, 0.4% Igepal CA-630, 1 mM MgCl2, 200 µg/ml lysozyme, 10 µg/ml pepstatin A, Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Each suspension was then supplemented with 0.1 mL of RPMI containing 1.25 mg mL−1 Liberase TM (Roche, 5401119001) and 20 mg mL−1 DNase I (Roche ...
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bioRxiv - Genomics 2023Quote: Fresh pinch biopsies were washed twice in DPBS before being coarsely chopped with a scalpel and transferred into 0.5 mL DPBS solution containing 0.1 WU ml−1 Liberase TH (Roche, 5401151001) and 0.5 U ml−1 RQ1 DNase I (Promega ...