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Citations for Roche :
1551 - 1600 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 1 % Nonidet-P 40) supplied with 1 tablet/50 mL c0mplete EDTA-free Protease Inhibitor Cocktail (Roche), per mg cell pellet and rotating on a wheel at 4°C for 15 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% NaDOC, 1% Triton X-100, 0.1% SDS, 0.25% sarkosyl, 1 mM DTT, protease inhibitors from Roche). The cells were sonicated on ice to shear DNA to 300-500 bp ...
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bioRxiv - Immunology 2020Quote: Tissue was harvested and incubated in a digestion cocktail containing 1 mg ml−1 collagenase A (Roche) and 30 μg ml−1 DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed sections were rehydrated and incubated with primary antibodies against ER (SP1; 1:1; Roche, Basel, Swiss), GATA3 (L50-823 ...
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bioRxiv - Microbiology 2022Quote: ... at a 1:1 ratio in a MagNA Pure 96-well deep-well extraction plate (06241603001, Roche), covered with a MagNA Pure Sealing Foil (06241638001 ...
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bioRxiv - Physiology 2023Quote: ... pregnant mice were anesthetized (1:1:0.16 Fentannyl [Fentadon, Dechra Vetinary Products UK], Midazolam [Hypnovel®, Roche, UK] and Ketamine [Ketavet ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EGTA and 1% Triton X-100 supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at RT with the secondary antibodies together with DAPI (1:5,000; Roche), all diluted in Dako Antibody Diluent ...
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bioRxiv - Genetics 2024Quote: ... 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate, 0.15 M NaCl, and 1×protease inhibitors, Roche) with slow rotation at 4 °C for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Rabbit anti-ALFA (1:1000, NanoTag Biotechnologies, RRID:AB_3075998) and Mouse anti-HA (1:1000, Roche, RRID: AB_514505) primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... followed by 1 hour antibody incubation (α-GFP, monoclonal mouse antibody, Roche, catalog no. 11814460001, 1:1000). After three washes with TBST for 10 min each ...
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bioRxiv - Neuroscience 2024Quote: ... The agarose resin was washed once with high salt buffer (50 mM Tris HCl pH 7.4, 1M NaCl, 1 mM EDTA, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail and RNase Inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting nuclei were resuspended in Wash Buffer (1 mL 1M HEPES pH 7.5, 1.5 mL 5M NaCl, 12.5 µL 2M spermidine, 1 Roche c0mplete Protease Inhibitor EDTA-free tablet with ddH2O to 50 mL ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 hour at room temperature and then probed with the HA-tag (Roche: 3F10; 1:4000) in PBS + 0.2% Tween-20 + 5% normal donkey serum overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.1 uL-1 of 1:20 diluted DNA was mixed with 1X Kapa HIFI Hotstart Readymix (Roche) and 200 nM primers ...
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bioRxiv - Microbiology 2024Quote: ... Lungs were homogenized with 1 mm silica beads in 1 mL PBS using a MagNA Lyser (Roche). Homogenization was carried out at 6,000 rpm for 60 s and chilled on ice for 5 min in-between runs ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 % NP-40 supplemented with 1 mM PMSF and 1 Complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland). The beads were washed three times with lysis buffer ...
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bioRxiv - Genetics 2024Quote: ... 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 1x Roche Complete Protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... 5’ RACE was performed with the 5’ RACE kit (Roche) using gene-specific reverse primers ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl of 5 x KAPA HiFi buffer (Roche, #KK2101) and 16.75 µl H2O ...
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bioRxiv - Microbiology 2021Quote: ... The infected PBMCs and CD4+ T cells were cultured at 37ºC in humidified air with 5% CO2 in the presence of 20 U/mL of IL-2 (Roche Diagnosis, Indianapolis, IN, USA), with various concentrations of S100A8 or S100A9 in RPMI-140 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... ITS (5 μg/mL insulin, 5 μg/mL transferrin and 5 ng/mL sodium selenite; Roche Diagnostics GmbH), and 5 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were extracted into a Triton X-100 buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM EDTA, 1% Triton X-100, supplemented with 1× protease inhibitors - Roche). Extracts were incubated on ice for 30 min then clarified by centrifugation at 12,000g for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was replaced with RIPA buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM MgCl2, 1% NP-40, 0.5% sodium deoxycholate, 1x complete protease inhibitor [Roche]) including 1% SDS ...
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bioRxiv - Developmental Biology 2021Quote: Cell pellets were incubated 20 minutes with RIPA lysis buffer (50 mM Tris-HCl pH7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 1X Roche COMPLETE Mini EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... for Western blot analysis probing for HA and FLAG epitopes using 1° antibody = 1:500 mouse αHA (Roche), or 1:500 mouse αFLAG (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... previously washed three times with RIPA 0.1% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA and 1X protease inhibitor cocktail; Roche). Anti-FLAG M2 Magnetic Beads (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Ground cell pellet was collected and resuspended end-over-end for 1 h in lysis buffer (160 mM HEPES pH 7.5, 400 mM NaCl, 0.25 mM PMSF, 1 Roche cOmplete™ Protease Inhibitor Cocktail tablet ...
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bioRxiv - Immunology 2022Quote: ... and then minced and dissociated in digestion buffer (RPMI containing collagenase (1 mg ml−1 collagenase D; Roche), DNase I (100 μg ml−1 ...
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bioRxiv - Neuroscience 2021Quote: ... containing Phosphatase (1 tablet per 3.3ml lysis buffer) and Protease (1 tablet per 16.67ml lysis buffer) inhibitor (Roche cOmplete tablets ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 viral load was determined using the COBAS® AmpliPrep/COBAS® TaqMan HIV-1 test (Roche). PBMCs were purified using BD Vacutainer® CPT™ Mononuclear cell preparation tubes (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... DNAse I (0.2 mg ml−1 dissolved in PBS) and Liberase (0.65 Wuensch units ml−1, both Roche, dissolved in HBSS supplemented with 8 % FCS) ...
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1mM CaCl2, 1% NP40, 0.5% Na Deoxycholate, 0.1% SDS 1% supplemented with protease inhibitor cocktail, Roche). 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1mM CaCl2, 1% NP40, 0.5% Na Deoxycholate, 0.1% SDS 1% supplemented with protease inhibitor cocktail, Roche). Clarified lysates were incubated with 10 µl of HPC4 coupled beads (Sigma ...
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bioRxiv - Genomics 2021Quote: Crosslinked MEFs per protocol above from 6-well dish were washed once in 2 mL PBS and collected in 1 mL cold NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 1 mM MgCl2, 1 mM DTT, 0.1% Triton X-100, Roche Protease Inhibitor Cocktail (Millipore (Burlington ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with maltose (20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 2.7 mM KCl, 0.1% NP40, 1 mM EDTA, 1mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with glutathione (50 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche; 3115828001) in lysis buffer (50 mM Tris HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.075% NP-40, 1x protease/phosphatase inhibitor cocktails, Roche) and incubated for 10 minutes at 4°C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.15M NaCl) for 1 hour at R/T and then incubated with anti-DIG antibody (Roche; 1:5000) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 mM CTP, 1 mM GTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP, pH 7.5; Roche/Sigma) and ∼40 U of either T7 (Roche/Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclear pellet was then resuspended in RIPA buffer (1X PBS, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS, supplemented with 1× protease inhibitor mixture Complete (Roche)) and chromatin was sheared to a range of 200-500bp by using a Bioruptor instrument (Diagenode ...
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bioRxiv - Biochemistry 2023Quote: ... and soluble FMS like Tyrosine kinase 1 (sFlt-1) on a Cobas e411 analyser (Roche Diagnostics, Mannheim, Germany).27 The serum hCG concentrations of the samples from the six individuals used in this study were compared against the respective LC-MS/MS derived values.