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Citations for Roche :
1501 - 1550 of 1565 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Proteins were separated by SDS-PAGE on 4-12% Bis-TRIS SDS-polyacrylamide gels (Novex, NP0322BOX) and analysis was done against primary antibodies α-HA (1:1000, Roche, 12CA5) or α-Tub2 (1:4500 ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexa-conjugated ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections were incubated with anti-HA antibody (1:400 dilution) followed by incubation with anti-rabbit-HQ (Roche; Cat#. 760-4815) and anti-HQ HRP (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexaconjugated ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Probes were detected by incubation overnight at 4°C in a 1:5000 dilution of Anti-DIG-AP antibody (Roche, Catalog# 11093274910) in Blocking Buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were stained with secondary antibodies (1:300) for 2 h at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). Cells were mounted with ProLong Diamond Antifade Mountant (Thermo P36970 ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were blocked in 5% (w/v) nonfat dry milk and probed with anti-HA antibodies (Roche 11867431001; 1:10,000 dilution), followed by horseradish peroxidase-coupled secondary anti-rat antibody (Sigma-Aldrich A9037 ...
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bioRxiv - Developmental Biology 2024Quote: ... then treated with blocking reagent (Genostaff) for 15 min and alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (Cytiva, clone BU-1) and rat anti-HA (Roche, clone 3F10) antibodies diluted 1:500 in block ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... to prepare for an hour long blocking in 10% lamb serum/TBST and an overnight incubation at 4°C in a 1% lamb serum containing Anti-Dig-AP antibody (Roche, 1:5000). The next morning ...
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bioRxiv - Neuroscience 2024Quote: ... specific digoxigenin (DIG)-labeled RNA probes and performed nitro blue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate color-reaction (BCIP) after wash and incubation with anti-digoxigenin antibody (catalog. no. 11093274910; Roche; RRID: AB_514497) as previously reported (Zempo et al. ...
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bioRxiv - Pathology 2024Quote: ... the aortic sections were incubated overnight at 4°C in a water-humidified chamber with anti-DIG-AP antibody diluted in blocking buffer (Roche, 1:500), followed by a three-time wash with TBST on the next day ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described in previous work (58) and detected with anti-digoxigenin-POD or anti-fluorescein-POD antibodies (1:2000, Roche/Sigma-Aldrich) blocked with 10% heat-inactivated Horse serum and 10% Western Blotting Blocking Reagent (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in 5% milk in PBS with 0.001% Tween-20 (PBST) before incubation with the specified antibodies prepared in 5% milk (GFP (Roche, 11814460001, 1:1,000), FLAG-HRP (Merck ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were the same as above and OmniMap anti-Ms HRP and anti-Rb HRP (Roche Cat#5269652001 and Cat#5269679001) secondary antibodies were coupled to OPAL520 (AKOYA Biosciences Cat# FP1487001KT) ...
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bioRxiv - Bioengineering 2024Quote: ... Specific cell types within these sections were identified using a series of primary antibodies: 1:500 rabbit anti-ERG (Roche, Basel, Switzerland) for endothelial cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated overnight at 4°C in primary antibody dilutions in freshly prepared BBT+ buffer (PBST + 1% BSA + 0.5 mM Spermidine + 2 mM EDTA + 1 large Roche complete EDTA-free tablets). Primary antibody was replaced with BBT+ buffer and quickly washed twice ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Fixed and permeabilized embryos were incubated with DIG or Fluorescein-labeled RNA probes and the labeled probes were detected by Alkaline Phosphatase (AP)-conjugated primary antibodies (Roche Cat# 11093274910, RRID:AB_514497) and Roche Cat# 11426338910 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... but with several differences from incubation with the anti-DIG antibody on: Incubation with blocking Buffer (2% Blocking Reagent from ROCHE in MABTween 1x) for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen) and SOX9 (1:10000 ...
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Microbiology 2020Quote: ... the sections were incubated with a primary antibody for 32 min at room temperature and then incubated with the reagent from an iVIEW DAB detection kit (Roche Diagnostics, Meylan, France). The sections were counterstained with hematoxylin and post-counterstained with bluing reagent ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were washed and the secondary antibodies applied (dilutions listed below) with 4′-6-diamidino-2-phenylindole (DAPI: Cat # 10-236-276-001, Roche Diagnostics, Indianapolis, IN) at 1:1,000 for 1 hour ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in anti-DIG POD (sheep anti-digoxigenin peroxidase conjugated antibody, RRID AB_514500; Roche, diluted 1:200 in the blocking buffer) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then mounted to glass slides and coverslipped with Vectashield Vibrance (Vector H-1700) Primary antibodies used for immunohistochemistry (IHC) are listed here with dilutions indicated in parentheses: rat anti-HA (Roche 11867423001, 1:500), chicken anti-GFP (Abcam ab13970 1:1500) ...
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bioRxiv - Immunology 2023Quote: ... following by the secondary antibody incubation and chromogenic detection with DISCOVERY Purple and Yellow kits (Ventana-Roche Diagnostics, #760-229 and # 760-239). These chromogenic dyes are covalently deposited and have unique spectra (83 ...
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bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were immediately covered with 20-60 µL primary antibody in Triton-Wash buffer (20mM HEPES pH 7.5,150mM NaCl, 2mM spermidine and Roche complete EDTA-free protease inhibitor) added dropwise ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each labeled probe was sequentially detected by incubation with a peroxidase-conjugated antibody against digoxigenin and peroxidase-conjugated anti-fluorescein (Roche, Indianapolis, IN, USA) followed by incubation with TSA-Alexa Fluor 555 and TSA-Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with 3% BSA in HBSS buffer for 20 min and incubated with a primary antibody (rat anti-HA, Roche, RD11867423001, 1:100) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...