Labshake search
Citations for Roche :
1501 - 1550 of 1792 citations for 6 Chloro 2 mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed twice in cold 1× PBS and harvested in 2% SDS in 50 mM HEPES buffer with freshly added protease and phosphatase inhibitors (Roche). Cell lysates were sonicated and centrifuged for 15 min at 12000 ×g to remove the insoluble fraction ...
-
bioRxiv - Molecular Biology 2022Quote: ... 300 mM NaCl, 20% glycerol, 2 mM MgCl2, 0.2 mM EDTA, 0.1% NP40, 0.5 mM DTT and 1x Roche protease inhibitor cocktail) on a rotating wheel at 4° C for 1 h in triplicates with GFP-Trap agarose beads (#gta-200 ...
-
bioRxiv - Microbiology 2022Quote: ... and lysed by incubation for 30 min on ice in non-denaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl, pH 7.4, 2% Triton X-100, and 1x EDTA-free protease inhibitor cocktail [Roche Complete]). For immunoblot assays ...
-
bioRxiv - Microbiology 2022Quote: ... GTP-binding Irgb6-T95D crystals diffracting to 1.68 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution containing 2 mM GTP (Roche) and a 0.5 μl of reservoir solution consisting of 0.2 M Sodium sulfate (Molecular Dimensions ...
-
bioRxiv - Plant Biology 2022Quote: Total protein extracts were prepared by re-suspending 100 mg of ground plant material in 100 μl of protein lysis buffer (50 mM Tris pH 8.0; 2% SDS; 10 mM EDTA; protease inhibitors (Roche (04693159001) cOmplete™ ...
-
bioRxiv - Neuroscience 2022Quote: One adult rat brain (2 g) was homogenized in nine volumes of 320 mM sucrose solution supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and Phosphatase Inhibitor Cocktail 1 and 2 (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNA was diluted to 2 ng/µL and 4 µL were added to 10 µL 2x FastStart Universal SYBR Green PCR Master (Roche). Each sample was run in triplicate using iTaq Universal SYBR Green Supermix (BioRad ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were extracted from the tissue powder or synaptogliosome pellets in 2% SDS (500 µl or 200 µl per sample, respectively) with EDTA-free Complete Protease Inhibitor (Roche), sonicated twice for 5 min or once for 5 min ...
-
bioRxiv - Neuroscience 2022Quote: ... The beads were then collected at 1000 g for 2 min and washed at least 3 times with lysis buffer coupled with protease inhibitor cocktail (Roche). After last wash and centrifugation ...
-
bioRxiv - Molecular Biology 2022Quote: ... The loci of interest were first amplified with 15 cycles of PCR from 2 μL (∼100 ng) of genomic DNA eluate using a 5 μL Kapa HiFi HotStart polymerase reaction (Roche). The first PCR was then diluted with 25 μL of DNAse-free water ...
-
bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were collected by centrifugation and re-suspended in Binding Buffer (PBS containing: 2 x cOmplete EDTA-free protease inhibitor cocktail [Roche] ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.1% NP-40, 1 mM DTT, 10 mM NaF, and EDTA-free protease inhibitor cocktail, Roche, Germany), and centrifugation at 20,000 × g for 3 min ...
-
bioRxiv - Cancer Biology 2022Quote: A total of 20-50 mg of snap-frozen melanoma tissues were pulverized by Geno/Grinder for 2 min at 1500 rpm and then fixed with 1% methanol-free formaldehyde plus protease inhibitor cocktails (Roche) for 10 min at room temperature and quenched by 125 μM glycine for 5 min at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.1% Cholesteryl Hemissucinate (CHS) (Avanti Pola lipids) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
-
bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl and 0.5% SMALP200 (CubeBiotech GmbH) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
-
bioRxiv - Microbiology 2024Quote: ... and lysed by incubation with nondenaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl [pH 7.4], 2% Triton X-100, and 1×EDTA-free protease inhibitor cocktail [Roche Complete]) for 30 min on ice ...
-
bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
-
bioRxiv - Immunology 2024Quote: ... digested three times (DMEM high glucose with 2% FBS, 100 µg/ml DNase I, and 100 µg/ml Liberase TM, Roche) or until no tissue fragments remained ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
-
bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
-
bioRxiv - Plant Biology 2023Quote: ... Glucose production rates were determined at 2 h using an Accu-Chek ®Aviva glucometer (Roche Diagnostics Scandinavia AB, Sweden). Monosaccharide (arabinose ...
-
bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended and homogenized in buffer B supplemented with 2 mM MgCl2 and 1x protease inhibitor (Roche). The protein was extracted by incubating with 1% GDN (Anatrace ...
-
bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
-
bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche) and 0.1 mg/mL DNAse I ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 5 mM EGTA, 1 mM DTT, 2 mM MgCl, and one EDTA-free protease inhibitor cocktail tablet; Roche) and sonicated in an ice bath for 6 minutes ...
-
bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... glands were minced into paste and incubated in 5 mL DME/F-12 medium (HyClone, #SH30023.1) with 2 mg/mL collagenase A (Roche #10103578001), 100 units/mL hyaluronidase (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... coli cell pellets was carried out by resuspending pellets in 50 mL of lysis buffer (50 mM NaPO4, 300 mM NaCl, 20 mM imidazole, 10 mM 2-mercapoethanol, protease inhibitor (Roche), DNAse (Sigma) ...
-
bioRxiv - Cell Biology 2023Quote: ... 4 ml of HBSS (without Ca2+ or Mg+) and 10 ml of collagenase solution [2 mg/ml collagenase H (11074059001; Roche), 0.1 mg/ml DNaseI (10104159001 ...
-
bioRxiv - Immunology 2023Quote: ... Tumors excised at day indicated in each figure were disaggregated mechanically and enzymatically with 2 mg/mL collagenase IV and 50 U/mL DNase I (Roche).
-
bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
-
bioRxiv - Neuroscience 2023Quote: Mouse brains at 5W were isolated and homogenized in homogenization buffer [(0.32 M sucrose, 10 mM HEPES, 2 mM EDTA and 1× complete protease inhibitor cocktail (Roche Diagnostics), pH 7.4)] ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in 4x pellet volume of sucrose buffer (10 mM HEPES/KOH pH 7.5.; 2 mM MgAc; 250 mM Sucrose, 1x Protease inhibitor cocktail [Roche, Germany]) and lysed with ~50 strokes in a tight-fit dounce homogenizer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed 2 × 5 min at 4 °C with cold 1 × PBS containing protease inhibitor cocktail (Roche, #4693132001), snap frozen and stored at −80°C until awaiting further processing ...
-
bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...